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Comparative Studies on Corynebacter1Um Pyogenes Toxin Formation in Monocultures and Mixed Cultures

Komparative undersøgelser over Corynebacterium pyogenes’ toksindannelse i mono- og blandingskulturer. Påvisning af en stimulerende effekt af Peptococcus indolicus og Stuart-Schwan kokker

The Demonstration of a Stimulating Effect of Peptococcus Indolicus and Stuart-Schwan Cocci

Acta Veterinaria Scandinavica volume 21, pages 438–447 (1980)Cite this article

Abstract

The growth and the toxin (i.e. hemolysin) producing capacity of Corynebacterium pyogenes were studied in monocultures and in co-cultures with 1 or more of the organisms frequently accompanying it in summer mastitis in cattle (Peptococcus indolicus, Stuart-Schwan cocci, Bacteroides melaninogenicus subsp. levii, Fusobacterium necrophorum and Streptococcus dysgalactiae) or with organisms seldom associated with summer mastitis (Streptococcus uberis, Streptococcus agalactiae, non-toxic staphylococci and Escherichia coli).

Pc. indolicus, and to some extent also Stuart-Schwan cocci, stimulated the growth as well as the hemolysin producing capacity of Gb. pyogenes (Table 1) while Str. dysgalactiae, Str. uberis, Str. agalactiae, E. coli and the majority of the staphylococci reduced these activities. Most F. necrophorum strains stimulated the growth, but not the hemolytic activity. With B. melaninogenicus the results were inconclusive.

The effect of Pc. indolicus appeared to be associated with the production of a filterable factor (Tables 2 and 3).

Mouse toxicity and hemolytic activity of culture filtrates were closely correlated (Table 4).

Sammendrag

Corynebacterium pyogenes’ vaekst og toksindanneise undersøgtes i monokulturer og i blandingskulturer med én eller fiere af de mikro-organismer, som typisk optræder sammen med den ved sommermastitis hos kvaeg (Peptococcus indolicus, Stuart-Schwan kokker, Bacteroides melaninogenicus subsp. levii, Fusobacterium necrophorum og Streptococcus dysgalactiae) samt med sàdanne, som sjaeldent påvises i sommermastitis-sekreter (Streptococcus uberis, Streptococcus agalactiae, ikke-toksiske stafylokokker og Escherichia coli).

Specielt Pc. indolicus, men også Stuart-Schwan kokker, stimulerede såvel vaekst som toksinproduktion (Tabel 1), medens Str. dysgalactiae, Str. uberis, Str. agalactiae, E. coli og flertallet af de under-søgte stafylokok-stammer hæmmede disse aktiviteter. Hovedparten af F. necrophorum stammerne stimulerede vaeksten, men ikke toksindannelsen. Forsøgene med B. melaninogenicus gav usikre resultater.

Effekten af Pc. indolicus viste sig at vaere associeret med danneise af en filtrerbar faktor (“peptococcus-faktor”) (Tabel 2 og 3).

Kulturfiltraters toksicitet for mus var snaevert korreleret med haemolysinindholdet (Tabel 4).

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  1. State Veterinary Serum Laboratory, Department for Jutland, Hangøvej 2, Århus, DK-8200, Denmark

    Gunner Høi Sørensen

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Sørensen, G.H. Comparative Studies on Corynebacter1Um Pyogenes Toxin Formation in Monocultures and Mixed Cultures. Acta Vet Scand 21, 438–447 (1980). https://doi.org/10.1186/BF03546876

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Acta Veterinaria Scandinavica

ISSN: 1751-0147