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Measurement of Staphylococcus Aureus Nuclease and Antinucleases
Applicability for The Assessment of Mastitic Milk
Bestemmelse av Staphylococcus aureus nuklease og antinukleaser. Anvendbarhet ved vurdering av mastittsekret.
Acta Veterinaria Scandinavica volume 21, pages 229–241 (1980)
Abstract
The concentration of the nuclease produced by Staphylococcus aureus and titres of antinucleases were determined in the udder secretions of 539 cows, most of them with clinical S. aureus mastitis. On average, the nuclease was detected in 85 % of the samples from mastitic quarters. In milk from most cows, antinucleases neutralize the activity of the nuclease, and a successful demonstration of this thermostable enzyme presupposed the inactivation of antibodies by heating the samples on boiling water-bath. The nuclease was demonstrated most frequently, and at highest concentration, in samples from cows with a severe mastitis and from cows which did not recover completely.
The test for nuclease, performed out both non-heated and heated quarter samples, and the antimuclease test, may supplement conventional laboratory methods. The advantages and limitations of the methods in the diagnosis of mastitis are discussed.
Sammendrag
Konsentrasjonen av nuklease produsert av Staphylococcus aureus og titeret av antinukleaser er blitt bestemt i jursekret fra 539 kyr, de fleste med klinisk mastitt forårsaket av Staphylococcus aureus. Nuklease ble i gjennomsnitt påvist i 85 % av prøvene av mastittsekret. I melk fra kyr vil det ofte være antinukleaser som nøytraliserer aktiviteten av nukleasen og en vellykket påvisning av det varmestabile enzymet forutsetter at antistoffer inaktiveres ved oppvarming på kokende vannbad. Nukleasen kan påvises oftest og i høyest konsentrasjoner i prøver fra kyr med alvorlig mastitt og fra kyr som ikke blir fullstendig helbredet.
Nukleasetesten utført på mastittprøver direkte og etter oppvarming, og antinukleasetesten kan være et supplement til vanlige laboratorieundersøkelser. Beskrivelsen omfatter også en omtale av fordelene og enkelte begreosninger ved metodene.
References
Giesecke, W. H. & L. W. Van den Heeuer: The diagnosis of bovine mastitis with particular reference to subclinical mastitis: A critical review of relevant literature. Onderstepoort J. vet. Res. 1974, 41, 169–212.
Giesecke, W. H. & M. H. Viljoen: The diagnosis of subclinical mastitis in lactating cows: A comparison of cytological methods and a monovalent radial immunodiffusion test. Onderstepoort J. vet. Res, 1974, 41, 51–74.
Gudding, R.: Heat stable nuclease in mastitic milk. Acta vet. scand. 1976, 17, 501–502.
Gudding, R.: An agar diffusion method for the determination of antibodies against Staphylococcus aureus deoxyribonuclease. Acta vet. scand. 1977, 18, 480–493.
Gudding, R.: Staphylococcal nuclease in udder secretions of cows with acute mastitis. Acta vet. scand. 1980 a, 21, 79–95.
Gudding, R.: Antibodies against staphylococcal and streptococcal nucleases in bovine blood serum and milk. Acta vet. scand. 1980 b, 21, 242–255.
Gudding, R.: Nuclease of Staphylococcus epidermidis isolated from mastitic milk. Production and some properties. Acta vet. scand. 1980 c, 21, 267–277.
Klastrup, O. & P. Schmidt Madsen: Nordiske rekommendationer vedrørende mastitisundersøgeIser af kirtelprøver. (Nordic recommendations concerning mastitis examinations of quarter samples). Nord. Vet.-Med. 1974, 26, 197–204.
Lachica, R. V. F., C. Genigeorgis & P. D. Hoeprich: Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 1971, 21, 585–587.
Sandvik, O.: The occurrence of antibodies against staphylococcal deoxyribonucleases in bovine milk. Acta vet. scand. 1975, 16, 140–142.
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Gudding, R. Measurement of Staphylococcus Aureus Nuclease and Antinucleases. Acta Vet Scand 21, 229–241 (1980). https://doi.org/10.1186/BF03546886
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DOI: https://doi.org/10.1186/BF03546886