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Nucleases of Some Udder Pathogenic Organisms

In Vivo and in Vitro Production

In vivo og in vitro nukleaseproduksjon hos enkelte jurpatogene mikroorganismer.

Abstract

The nuclease activity in quarter milk samples from cows with mastitis, and in vitro nuclease production by some udder pathogenic microorganisms were tested. Results concerning Staphylococcus aureus are, however, dealt with in a separate paper. Streptococcus dysgalactiae was found to be a vigorous and frequent producer of nuclease both in vivo and in vitro. The production by S. agalactiae was quantitatively inferior to that by S. dysgalactiae. Nearly half of the S. epidermidis strains were found to excrete a nuclease, and this enzyme was also demonstrated in the milk of cows with S. epidermidis mastitis.

Comparatively high nuclease activity was recorded in the udder secretions of some cows with summer mastitis. All the tested strains of Gorynebacterium pyogenes were nuclease positive when tested by agar plate methods.

Sammendrag

Innholdet av nuklease i kjertelprøver fra kyr med mastitt og in vitro produksjon av nuklease hos enkelte mastittbakterier er blitt undersøkt. Resultater vedrørende Staphylococcus aureus er imidlertid omtalt i en egen artikkeL De fleste stammene av Streptococcus dysgalactiae produserte nuklease in vivo og in vitro og produksjonen av enzymet hos denne bakterien var generelt sterkere enn hos de fleste andre av de undersøkte bakteriene. Streptococcus agalactiae produserte mindre nuklease enn S. dysgalactiae. Nesten halvparten av S. epidermidis-stammene var nuklease positive og dette enzymet ble også påvist i melk fra enkelte kyr med mastitt forårsaket av S. epidermidis.

I jursekret fra enkelte kyr med sommermastitt ble det funnet relativt høy nukleaseaktivitet. Alle de undersøkte stammene av Corynebacterium pyogenes produserte nuklease.

References

  • Bernandi, G.: Spleen acid deoxyriboniuclease. In Boyer, P. D. (ed.): The Enzymes. Acad. Press;, New York and London 1971, 4, 271–287.

  • Birnboim, H. C.: Cellular site in Bacillus subtilis of a nuclease which preferentially degrades single-stranded nucleic acids. J. Bact. 1966, 91, 1004–1011.

    Article  CAS  Google Scholar 

  • Cazin jr., J., T. R. Kozel, D. M. Lupan & W. R. Burt: Extracellular deoxyribonuclease production by yeasts. J. Bact. 1969, 100, 760–762.

    Article  CAS  Google Scholar 

  • Gudding, R.: Heat stable nuclease in mastitic milk. Acta vet. scand. 1976, 17, 501–502.

    CAS  PubMed  Google Scholar 

  • Gudding, R.: The demonstration and characterization of deoxyribonu-cleases of streptococci group A, B, C, G and L. Acta vet. scand. 1979 a, 20, 102–121.

  • Gudding, R.: DNases in milk and blood sera from different species. Acta vet. scand. 1979b, 20, 404–416.

  • Gudding, R.: Staphylococcal nuclease in udder secretions of cows with acute mastitis. Acta vet. scand. 1980 a, 21, 79–95.

  • Gudding, R.: Measurement of Staphylococcus aureus nuclease and antinucleases. Applicability for the assessment of mastitic milk. Acta vet. scand. 1980 b, 21, 229–241.

  • Gudding, R.: Antibodies against staphylococcal and streptococcal nucleases in bovine blood serum and milk. Acta vet. scand. 1980 c, 21, 242–255.

  • Gudding, R.: Nuclease of Staphylococcus epidermidis isolated from mastitic milk. Production and some properties. Acta vet. scand. 1980 d, 21, 267–277.

  • Holmberg, O.: Staphylococcus epidermidis isolated from bovine milk. Acta vet. scand. 1973, Suppl. 45, 1–144.

  • Klastrup, O. & P. Schmidt Madsen: Nordiske rekommendationer ved-rørende mastitisundersøgelser af kirtelprøver. (Nordic recom-mendatioins concerning mastitis examinations of quarter samples). Nord, Vet.-Med. 1974, 26, 197–204.

    CAS  Google Scholar 

  • Lachica, R. V. F., C. Genigeorgis & P. D. Hoeprich: Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 1971, 21, 585–587.

    Article  CAS  Google Scholar 

  • Lehman, I. R.: Bacterial deoxyribonucleases. In Boyer P. D. (ed.): The Enzymes. Acad. Press, New York and London 1971, 4, 251–270.

  • Porschen, R. K. & S. Sonntag: Extracellular deoxyribonuclease production by anaerobic bacteria. Appl. Microbiol. 1974, 27, 1031–1033.

    Article  CAS  Google Scholar 

  • Schreier, J. B.: Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens. Amer. J. clin. Path. 1969, 51, 711–716.

    Article  CAS  Google Scholar 

  • Streitfeld, M. M., E. M. Hoffmann & H. M. Janklow: Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J. Bact. 1962, 84, 77–80.

    Article  CAS  Google Scholar 

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Gudding, R. Nucleases of Some Udder Pathogenic Organisms. Acta Vet Scand 21, 256–266 (1980). https://doi.org/10.1186/BF03546888

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