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Experimental Maedi Infection in Sheep
2. Antibody Response
Experimenten maedi infektion hos får. 2. Bildningen av antikroppar. Alla fyra får som inokulerats via luftvägarna med 7×106 TCID50 av maedi M88 stammen påvisade komplement bindande (CF) antikroppar inom tre månader efter inokulationer, och en gradvis ökning av CF-titern upptäcktes under det första året. Under det påföljande året har CF antikropptitern trots en ringa fluktuation bibehållits och varierat mellan 64 og 256. Virusneutraliserande aktivitet mot maedi M88 stammen upptäcktes i sera av alia intrapulmonalt inokulerade får inom åtta månader efter inokulationen. Titrarna har bibehållits eller ökat lindrigt. Nivån av titrarna, som släckte sig från 8 till 256, har klart varierat mellan individerna.
Acta Veterinaria Scandinavica volume 21, pages 124–133 (1980)
Abstract
All 4 sheep inoculated via the respiratory tract with 7×106 TCID50 af maedi M88 strain developed complement fixing (CF) antibodies within 3 months after inoculation, and a gradual rise in CF titers was found during the first year. The antibody titers have been maintained, though with some fluctation, through the following year, and the titers vary from 64 to 256. Virus neutralizing activity against maedi M88 strain was detected in the sera of all intrapulmonarily inoculated sheep within 8 months after inoculation. Titers have been maintained or have slightly increased. The level of titers, ranging from 8 to 256, was clearly different between individual sheep.
One of the 4 sheep inoculated intracerebrally with 5×105 TCID50 of maedi M88 strain developed CF antibodies 1 month after inoculation, but no neutralizing antibodies until death 11 months after inoculation. The rest of the intracerebrally inoculated sheep displayed no evidence of CF or neutralizing antibodies within 18 months after inoculation in spite of numerous virus isolations from peripheral blood leukocytes. The absence of antibodies might perhaps be attributed to phenomena such as differences in tropism, provirus state, immunological tolerance and size of inoculum.
One sheep hyperimmunized with repeated s.c. and i.v. injections of maedi M88 strain developed high CF antibody titers but lower neutralizing antibody titers.
The 2 uninoculated control sheep developed no CF or neutralizing antibodies within 18 months after inoculation.
Sammanfattning
Ett av fåren som inokulerades intracerebralt med 5×105 TGID50 av maedi M88 stammen påvisade CF antikroppar en månåd efter inokulationen men inga neutraliserande antikroppar till dess död elva månader efter inokulationen. Hos de andra intracerebralt inokulerade fåren har CF eller neutraliserande antikroppar inte påvisats inom 18 månader efter inokulationen trote många virus isoleringar från perifera blod leukocyter. Förklaringen till bristen på antikroppar kunde möjligen ligga i sådana fenomen som olikheter i tropism, provirus tillstånd, immunologisk tolerans eller storleken av inokulumet.
Ett får hyperimmuniserat med s.c. och i.v. injektioner av maedi M88 stammen påvisade höga CF antikropptitrar men lågre neutraliserande antikropptitrar.
Två oinokulerade kontrollfår påvisade inga CF eller neutraliserande antikroppar inom 18 månader efter inokulationen.
References
De Boer, G. F.: Antibody formation in zwoegerziekte, a slow infection in sheep. J. Immunol. 1970, 104, 414–422.
Gudnadottir, M.: Host-virus interaction in maedi infected sheep. In Lung Tumours in Animals. Proc. 3rd Int. Gonf. Cancer, Perugia 1966, 381–391.
Gudnadottir, M.: Visna-maedi in sheep. Progr. med. Virol. 1974, 18, 336–349.
Gudnadottir, M. & K. Kristinsdottir: Complement-fixing antibodies in sera of sheep affected with visna and maedi. J. Immunol. 1967, 98, 663–667.
Gutlip, R. C., T. A. Jackson & G. A. Laird: Immunodiffusion test for ovine progressive pneumonia. Amer. J. vet. Res. 1977, 38, 1081–1084.
Haase, A. T., L. Stowring, O. Narayan, D. Griffin & D. D. Price: Slow persistent infection caused by visna virus: Role of host restriction. Science 1977, 195, 175–177.
Narayan, O., D. E. Griffin & A. M. Silverstein: Slow virus infection: Replication and mechanisms of persistence of visna virus in sheep. J. infect. Dis. 1977, 135, 800–806.
Pálsson, P. A.: Maedi and visna in sheep. In Kimberlin, R. H. (ed.): Slow Virus Diseases of Animals and Man, Frontiers in Biology. Vol. 44. North Holland Publishing Co., Amsterdam and Oxford 1976, 17–43.
Petursson, G.: Comment/Chapter 3. In ter Meulen, V. & Katz, M. (ed.): Slow Virus Infections of the Central Nervous System. Springer-Verlag, New York, Heidelberg, Berlin 1977, 71–72.
Petursson, G., N. Nathansson, G. Georgsson, H. Panitch & P. A, Palsson: Pathogenesis of visna 1. Sequential virologic, serologic and pathologic studies. Lab. Invest. 1976, 35, 402–412.
Sihvonen, L., T. Estola & J, Tuomi: Experimental maedi infection in sheep. 1. Detection of virus, cliniical course, histopathology. Acta vet. scand. 1980, 21, 113–123.
Terpstra, C. & C. F. De Boer: Precipitation antibodies against maedivisna virus in experimentally infected sheep. Arch. ges. Virus-forsch. 1973, 43, 52–62.
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This work was supported by the Finnish Academy.
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Gazia, N., Wegger, I. Experimental Maedi Infection in Sheep. Acta Vet Scand 21, 124–133 (1980). https://doi.org/10.1186/BF03546907
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DOI: https://doi.org/10.1186/BF03546907