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Cryopreservation of Boar Semen

Djupfrysning av galtsperma. I. En litteraturöversikt

I: A literature review

Acta Veterinaria Scandinavica volume 32, pages 431–453 (1991)Cite this article

Abstract

The present review summarizes information concerning the methods available to cryopreserve boar semen, covering the historical background, cryobiology and cryoprotecting considerations, technological developments and recent advances in cryopreservation methodologies. Successful methods for cryopreservation of boar semen have not been achieved despite numerous efforts world wide. Improvements in semen preservation technologies have been deterred by lack of in vitro methods that can accurately predict in vivo fertilizing capacity of frozen boar semen. The cell membrane is of crucial importance with regard to freeze-thaw survival of spermatozoa. It is important to optimize the survival of the plasma membrane as this is a non homogenous entity both in structure and function. The boar sperm membrane exhibits extreme sensitivity to freezing treatment. Freezing and thawing results in considerable changes in electrolyte dynamics and damages have mainly been associated with alterations in the head membranes especially at thawing. To date fruitless efforts have been carried out to find a cryoprotectant for the spermatozoa membranes and glycerol still continues to be used despite its harmful effects to the membranes.

Sammanfattning

Denna översiktsartikel sammanfattar och diskuterar de tillgängliga djupfrysningsmetoderna av galtsperma. Den ger en sammanfattning av den historiska bakgrunden, grundläggande kunskaper i kryobiologi, frysskyddande medel samt se senaste framstegen i djupfrysningstekniken. Tillräckligt goda djupfrysningsmetoder har inte presenterats trots stora ansträngningar i olika länder. In vitro metoderna att värdera kvaliteten på den djupfrysta sperman har inte varit tillräckligt säkre för att samtidigt värdera befruktningsförmågan. Cellmembranen är av utomordentligt stor betydelse för infrysning-upptiningsmomenten. Spermiecellmembranen är inte en enhetlig struktur utan varierar både i morfologi och funktion och är mycket känslig för låga temperaturer. Frysning och upptining medför förändringar i elektrolyttransporten över membranen speciellt i den del som täcker spermiehuvudet. Fortfarande är glycerol den frysskuddande substans som används trots dess skadliga inverkan på cellmembranen hos galtspermier.

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  1. Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, P. O. Box 7039, S-75007, Uppsala, Sweden

    C. O. Bwanga

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Bwanga, C.O. Cryopreservation of Boar Semen. Acta Vet Scand 32, 431–453 (1991). https://doi.org/10.1186/BF03546944

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Acta Veterinaria Scandinavica

ISSN: 1751-0147