- Published:
Sensitivity and Specificity of Different Methods for the Isolation of Salmonella from Pigs
Sensitivitet og specificitet afforskellige metoder til påvisning af salmonellabakterier fra svin
Acta Veterinaria Scandinavica volume 32, pages 473–481 (1991)
Abstract
Three different selective enrichment media, Rappaport-Vassiliadis broth (RV), selenite broth (SB) and Müller-Kauffmann tetrathionate broth (MKTB), in combination with plating on modified brilliant green agar (BGA), were compared for the isolation of Salmonella from samples of pig feces. These conventional methods were also compared with a new ELISA kit in conjunction with RV and SB enrichment. Of the conventional methods, enrichment in RV had a higher sensitivity and selectivity than SB and MKTB. Recovery of S. typhimurium from MKTB was significantly poorer than recovery of other serotypes. The combination of RV enrichment and ELISA was as good as the conventional method involving RV enrichment, with a similar high sensitivity and specificity.
Sammendrag
Tre salmonella opformeringsmetoder, Müller-Kauffmann tetrathionat (MKTB), selenit bouillon (SB) og Rappaport-Vassiliadis bouillon (RV), in-kuberet ved henholdsvis 43°C, 37°C og 42°C, blev sammenlignet med hensyn til sensitivitet og selektivitet ved undersøgelse af gødiningsprøver fra svin. Efter selektiv opformering blev salmonella påvist, dels traditionelt ved udsæd på modificeret brilliantgrøn agar (BGA), og dels immunologisk ved hjælp af et nyt kommercielt ELISA kit. RV havde en statistisk signifikant højere sensitivitet og selektivitet end både SB og MKTB. Opformering i MKTB gav en statistisk signifikant lavere påvisning af S. typhimurium end af andre serotyper. Den anvendte ELISA teknik, kombineret med opformering i RV havde en lige så høj sensitivitet og specificitet som ved traditionel udsæd fra RV-mediet.
References
Bailey JS, Cox NA, Thomson JE: Efficiency of selenite cystine and TT enrichment broths for the detection of Salmonella. J. appl. Bacteriol. 1981, 51, 409–414.
D’Aoust JY: Salmonella. In: Doyle MP (ed): Foodborne Bacterial Pathogens. Marcel Dekker, New York 1989.
Haddock RL: Efficacy of examining rectal swabs to detect swine Salmonella carriers. Amer. J. vet. Res. 1970, 31, 1509–1512.
Harvey RWS, Price TH, Morgan J: Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975. J. Hyg. (Camb.) 1977, 78, 439–448.
Lund C: Personal communication, 19 ...
Martin SW, Meek AH, Willeberg P: Veterinary Epidemiology. Iowa State University Press, Ames, Iowa, USA 1987, 367 pp.
Munch B, Bonde Larsen A: Veterinær forskning, overvågning og rådgivning i forbindelse med etablering og drift af biogasfællesanlæg. (Veterinary research, surveillance and councelling in relation to the establishment and operation of communal biogas digestors). Report to the Danish Veterinary Service, 1990, 188 pp.
Peterz M, Wiberg C, Norberg P: The effect of incubation temperature and magnesium chloride concentration on growth of Salmonella in homemade and in commercially available dehydrated Rappaport-Vassiliadis broths. J. Appl. Bacteriol. 1989, 66, 523–528.
SAS Institute Inc.: SAS/STAT User’s guide, Release 6.03 Edition. SAS/Graph User’s guide, Release 6.03 Edition.
Vassiliadis P, Trichopoulos D, Papadakis J, Ka-lapothaki V, Zavitsos X, Serie C: Salmonella isolation with Rappaports’s enrichment medium of different compositions. Zbl. Bakt., I. Abt. Orig. B. 1981, 173, 382–389.
Vassiliadis P: The Rappaport-Vassiliadis (RV) enrichment medium for the isolation of Salmonellas: An overview. J. Appl Bacteriol. 1983, 54, 69–76.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eager, F., Petersen, J. Sensitivity and Specificity of Different Methods for the Isolation of Salmonella from Pigs. Acta Vet Scand 32, 473–481 (1991). https://doi.org/10.1186/BF03546947
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03546947