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The Effect of Lactic Acid Sprays on Campylobacter jejuni Inoculated onto Poultry Carcasses

Effekten av melkesyre på Campylobacter jejuni inokulert på fjørfeslakt

Abstract

Spraying poultry carcasses with 1 % lactic acid 10 min after inoculation with Campylobacter jejuni, resulted in a significant reduction in the number of the bacteria after 4 h at 4°C. Some of the inoculated cells, however, survived for at least 144 h. Spraying 10 min after inoculation with 2% lactic acid, totally eliminated all inoculated C. jejuni within 24 h. On the other hand, spraying 24 h after inoculation, with either 1 % or 2 % lactic acid did not eliminate all the bacteria. Inoculated C. jejuni on poultry carcasses not sprayed with lactic acid, survived at 4°C throughout the sampling period (up to 144 h) and showed little tendency to decrease in number even when the carcasses started to deteriorate. Resident Campylobacters on poultry carcasses were significantly reduced by the lactic acid treatment. Frozen and thawed chickens appeared to show a graying of the skins immediately after spraying with lactic acid, slightly stronger with 2 % lactic acid, but the colour reverted to normal after 24 h. We were not able to observe any colour change on the fresh broiler chickens after lactic acid treatment. Our results indicated that lactic acid had a significant bactericidal effect on C. jejuni on both naturally and artificially contaminated poultry carcasses. This effect, however, became manifest only several hours after acid treatment.

Sammendrag

Artikkelen omtaler effekten av melkesyre på Campylobacter jejuni på overflaten av fjørfeslakt. Resultatene viste at spraying av slaktene med 1 % melkesyre 10 min etter inokulasjon med C. jejuni ga en signifikant reduksjon av antallet bakterier etter 4 timer ved 4°C. Noen av de inokulerte bakteriene viste seg imidlertid å kunne overleve minst 144 t. Bruk av 2 % melkesyre så ut til å eliminere alle C. jejuni innen 24 t. Hvis melkesyrebehandlingen derimot ble utført 24 t etter inokulering, ble det ikke observert noen tilsvarende eliminasi on av bakteriene hverken med 1 % eller 2 % melkesyre. På slakt som ikke ble behandlet med melkesyre, overlevde C jejuni gjennom hele forsøksperioden (144 t) ved 4°C. I løpet av denne tiden viste bakterien bare liten tendens til reduksjon i antall selv om slaktene viste tegn på begynnende forråtnelse. Fjørfeslakt som var naturlig infisert med Campylobacter, viste en signifikant reduksjon av antall Campylobacter-bakterier etter melkesyrebehandling. Melkesyrebehandlingen forte til en grå misfargning av de frosne slaktene, særlig ved bruk av 2 % melkesyre, men denne fargen forsvant etter 24 timer. Ferske fjørfeslakt viste ingen fargeforandring etter melkesyrebehandling. Resultatene viser at melkesyre har en signifikant baktericid effekt overfor C. jejuni på både naturlig og kunstig infisert fjørfeslakt. Denne effekten viste seg imidlertid forst mange timer etter behandling.

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Cudjoe, K.S., Kapperud, G. The Effect of Lactic Acid Sprays on Campylobacter jejuni Inoculated onto Poultry Carcasses. Acta Vet Scand 32, 491–498 (1991). https://doi.org/10.1186/BF03546949

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