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A Modification of the Indirect Immunofluorescence Test for Detection of Ehrlichia phagocytophila Antibodies

En modifikasjon av indirekte immunofluorescens test for påvisning av antistoffer mot Ehrlichia phagocytophila

Acta Veterinaria Scandinavica volume 32, pages 499–502 (1991)Cite this article

Abstract

A modified technique for production of antigen and performance of the test is described. A suspension of infected neutrophils was directly applied to multiwell slides. Multichannel pipettes may be used for dilution and application of sera. The modification inreases the capacity both by production of the antigen and by performance of the test. This paper also gives a quantitative determination of the antibodies.

Sammendrag

En modifisert teknikk for produksjon av antigen og utførelse av testen er beskrevet. En suspension med infiserte nøytrofile granulocytter avsettes direkte på objektglass med 10 teflonbrønner. Multikanal pipetter kan nyttes til fortynning og applikasjon av sera. Modifíkasjonen øker kapasiteten både ved produksjon av antigen og ved utførelsen av testen. Artikkelen inneholder også en kvantitativ bestemmelse av antistoffer.

References

  • Carlson GP, Kaneko JJ: Isolation of leucocytes from bovine peripheral blood. Proc. Soc. exp. Biol. Med. 1972, 142, 853–856.

    Article  Google Scholar 

  • Christensson D: Improvement of the teflonized slides used in the immunofluorescent antibody technique. Acta vet. scand. 1986, 27, 296–298.

    CAS  PubMed  Google Scholar 

  • Foggie A, Lumsden WHR, McNeillage JC: Preservation of the infectious agent of tick-borne fever in the frozen state. J. comp. Path. 1966, 76, 413.

  • Paxton EA, Scott GR: Detection of antibodies to the agent of tick-borne fever by indirect immunofluorescence. Vet. Microbiol. 1989, 21, 133–138.

    Article  CAS  Google Scholar 

  • Scott GR: Tick-borne fever in sheep. Vet. Ann. 1984, 24, 100–106.

    Google Scholar 

  • Snodgrass DR, Ramachandran S: A complement fixation test for tick-borne fever of sheep. Br. vet. J. 1971, 127, 44–46.

    Article  Google Scholar 

  • Tuomi J: Experimental studies on bovine tickborne fever (4). Immunofluorescent staining of the agent and demonstration of antigenic relationship between strains. Acta path, microbiol. scand. 1967, 71, 101–108.

    Google Scholar 

  • Webster KA, Mitchell GBB: Use of counter immunoelectrophoresis in detection of antibodies to tickborne fever. Res. Vet. Sci. 1988, 45, 28–30.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Large Animal Clinical Sciences, Medical Section, Norwegian College of Veterinary Medicine, P. O. Box 8146, Dep, N-0033, Oslo 1, Norway

    Frøydis Hardeng

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  1. Frøydis Hardeng
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Hardeng, F. A Modification of the Indirect Immunofluorescence Test for Detection of Ehrlichia phagocytophila Antibodies. Acta Vet Scand 32, 499–502 (1991). https://doi.org/10.1186/BF03546950

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  • DOI: https://doi.org/10.1186/BF03546950

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147