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Inoculation of Infectious Pancreatic Necrosis Virus Serotype Sp did not cause Pancreas Disease in Atlantic Salmon (Salmo salar L.)
Inokulering av Atlantiske laks (Salmo salar L.) med infeksiøst pankreas nekrose virus, serotype Sp frambrakte ikke pancreas disease
Acta Veterinaria Scandinavica volume 32, pages 503–510 (1991)
Abstract
Atlantic salmon were selected from a fish farm with no previous record of pancreas disease (PD) or infectious pancreatic necrosis virus (IPNV) infection. Groups of fish were inoculated with either IPNV (strain Sp) from cell culture, organ material from fish with PD or control material as phosphate-buffered saline (PBS). Virological, histological and immunohistochemical examinations were carried out throughout the experiment. None of the fish died or showed clinical symptoms of PD. Histological examination revealed no pathological changes, and immunohistochemical studies were negative. Virus was isolated only sporadically from the group inoculated with organ material, whereas it was isolated consistently from the group inoculated with virus propagated in cell culture, as well as from in-contact control fish after the first week. In a latent carrier test, changes were entirely lacking in the first mentioned group, and were only slight in the last mentioned group. The data suggest that PD is not a transmissible disease, and that IPNV isolated from a PD outbreak does not play any part in the etiology of this disease.
Sammendrag
Pancreas diseases (PD) er en sykdom på oppdrettslaks som har hatt en betydelig spredning i de senere år. En infeksiøs etiologi har blitt foreslåt, men det er ikke identifisert noe infeksiøst agens. Atlantisk laks i mottagelig alder for PD ble brukt i et smitteforsøk. Laksen kom fra et oppdrettsanlegg uten noen forhistorie med PD eller isolasjon av IPNV. Tre grupper med fisk ble enten inokulert med a) IPNV type Sp (isolert fra fìsk med PD), b) organmateriale fra físk med PD, eller c) negativi kontrollmateriale. Virologiske, histologiske og immunohistokjemiske undersøkelser ble utført gjennom hele fors0ket. Ingen av forsøksfiskene døde eller viste tegn på patologiske forandringer, videre var immunohistokjemiske undersøkelser negative. IPNV ble isolert fra gruppen som ble inokulert med virus og fra kontrollfisk som var i samme kar som disse under hele forsøket som totalt varte i 75 dagar. Fra gruppen som ble inokulert med organmateriale ble IPNV kun isolert sporadisk. En latent carrier test utført på dag 67 ga ingen forandringer hos fìsk inokulert med organmateriale eller hos fisk inokulert med virus, mens en moderat stigning i IPNV titeret ble funnet i kontrollene til sistnevnte gruppe. Konklusjonen fra smittefor-søket ble at det ikke var funnet bevis for at pancreas disease er en smitsom sykdom. IPNV isolert fra fisk under et PD utbrudd synes heller ikke å ha noen etiologisk betydning for denne sykdommen.
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Rimstad, E., Poppe, T., Evensen, Ø. et al. Inoculation of Infectious Pancreatic Necrosis Virus Serotype Sp did not cause Pancreas Disease in Atlantic Salmon (Salmo salar L.). Acta Vet Scand 32, 503–510 (1991). https://doi.org/10.1186/BF03546951
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DOI: https://doi.org/10.1186/BF03546951