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ATP Content and Sperm Motility of Extended Bovine Semen under Different Storage Conditions

A TP innehålt och spermiemotilitet i spädd sperma under olika förvaringsfòrhållanden

Acta Veterinaria Scandinavica volume 32, pages 511–518 (1991)Cite this article

Abstract

The effects of incubation temperature (+20°C vs +35°C) and media type on the ATP content and motility of spermatozoa were determined in fresh bovine semen in order to develop a method for assaying post-thaw quality. Semen was obtained from 3 bulls at 2 occasions. The spermatozoa were washed using a Ficoll-containing medium before being resuspended in each of 4 different media (I. 0.9 % NaCl; II. Trisbuffer solution; III. seminal plasma; IV. seminal plasma + Tris-buffer solution) and incubated for 6 h. The least-squares means for ATP content were higher (p ≤ 0.05) at +20°C than +35°C for all media except no. I.

By contrast, the least-squares means for sperm motility were higher (p ≤ 0.05) at +35°C than at +20°C in media II and III. A decrease over time in ATP content and motility at both temperatures was also observed.

The single most important factor responsible for changes in ATP content and sperm motility was the temperature and the medium, respectively.

Sammanfattning

Effekten av inkuberingstemperatur (+20°C och +35°C) och mediatyp på ATP-innehållet och spermiemotiliteten undersöktes i farsk tjursperma i syfte att utveckla en metod för bedömning av spermakvaliteten efter upptining. Sperma från 3 tjurar samlad vid 2 olika tillfallen användes. Spermierna tvättades med hjälp av centrifugering genom en Ficoll-lösning innan de späddes med 4 olika medier (I. 0.9 % NaCl; II. Tris-buffert; III. seminalplasma; IV. seminalplasma + Tris-buffert) och inkuberades under 6 t. Least-squares means för ATP var högre (p ≤ 0.05) vid +20°C jämfört med vid +35°C, i alia medier utom i medium I. Däremot var least-squares means för spermiemotiliteten högre (p ≤ 0.05) vid +35°C jämfört med vid +20°C i medium II och III. En nedgång över tiden i både ATP-innehållet och spermiemotiliteten ägde rum under inkuberingen vid båda temperaturerna. Den viktigaste enskilda orsaken till förändringar i ATP-innehållet och spermiemotiliteten var temperaturen respektive mediet.

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  1. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden

    Lennart Söderquist

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Söderquist, L. ATP Content and Sperm Motility of Extended Bovine Semen under Different Storage Conditions. Acta Vet Scand 32, 511–518 (1991). https://doi.org/10.1186/BF03546952

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Acta Veterinaria Scandinavica

ISSN: 1751-0147