Skip to main content
Download PDF
  • Published:

Evaluation of Three Slide Agglutination Tests for Rapid Identification of Staphylococcus aureus

Bedömning av tre “slide” agglutinationstester för snabb identifiering av Staphylococcus aureus

Acta Veterinaria Scandinavica volume 32, pages 543–549 (1991)Cite this article

Abstract

Three slide agglutination tests for identification of Staphylococcus aureus were compared. The agglutination tests used for evaluation were Staphaurex (Wellcome Diagnostics), Staphyslide-Test (BioMerieux), and ANI S. aureus TEST (Ani Biotech Oy). A total of 347 isolates were analyzed, including 288 strains of S. aureus, 49 of S. epidermis, 11 of S. intermedius, 12 strains of other staphylococci and 14 non-staphylococcal strains. One hundred of the S. aureus strains were isolates from cases of food poisoning, 129 from mastitis and 59 from other clinical cases. The sensitivities of the tests were also compared using diluted suspensions of S. aureus strains and with purified Protein A dilutions. The results showed that the sensitivities of the tests were 98.6 %, 97.9 % and 99.0 % for Staphaurex, Staphyslide-test and ANI S. aureus TEST, respectively. The specificities were 100 % for the Staphyslide test and 98.8 % for both the ANI S. aureus TEST and the Staphaurex test. The sensitivities measured with diluted S. aureus strain suspensions and Protein A solutions were equal with the Staphaurex and ANI S. aureus TEST.

All the agglutination tests studied proved to be practical, easy to use and accurate for the rapid identification of S. aureus strains from culture isolates.

Sammanfattning

Tre slide agglutinationstester för identifiering av Staphylococcus aureus jämfördes. Följande 3 tester undersöktes: Staphaurex (Wellcome Diagnostics), Staphyslide-Test (Bio Merieux) och ANI S. aureus Test (Ani Biotech Oy).

Sammanlagt 347 isolationer analyserades innefattande 288 stammar av S. aureus, 49 av S. epidermis, och 11 av S. intermedius, 12 stammar av andra staphylococci och 14 non-staphylococci stammar. Hundrade av S. aureus stammarna var isolerade ur matförgiftningsfall, 129 ur mastitis och 59 ur andra kliniska fall. Även testernas känslighet jämfördes genom att använda diluterade suspensioner av S. aureus stammar och med renade Protein A dilutioner.

Resultaten visade att testkänsligheten var 98,6 %, 97,9% och 99,0% för Staphaurex, Staphyslide-Test och ANI S. aureus Test i nämnd ordning. Specifiteten för Staphyslide Testet var 100% och för ANI S. aureus Testet 98,8 % vardera. Känslighet mätt med diluterade suspensioner av S. aureus stammar och Protein A lösningar var lika för Staphaurex och ANI S. aureus Test.

Alla undersökta tester visade sig vara praktiska, lätta att använda och exakta för snabb identifiering av S. aureus stammar i odlingsprov.

References

  • Baker JS, Bormann MA, Boudreau DH: Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus. J. clin. Microbiol. 1985, 21, 726–729.

    Article  CAS  Google Scholar 

  • Bergdoll MS, Cross BA, Reiser RF, Robbins RN, Davis JP: A new staphylococcal enterotoxin, enterotoxin F, associated with toxic shock syn drome in Staphylococcus aureus isolates. Lancet 1981, i, 1017–1021.

  • Berke A, Tilton RC: Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus. J. clin. Microbiol. 1986, 23, 916–919.

    Article  CAS  Google Scholar 

  • Brown WJ: Comparison of a yellow latex reagent with other agglutination methods for the identification of Staphylococcus aureus. J. clin. Microbiol. 1986, 23, 640–642.

    Article  CAS  Google Scholar 

  • Essers L, Radebold K: Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J. clin. Microbiol. 1980, 12, 641–642.

    Article  CAS  Google Scholar 

  • Flesland O: Comparison of two agglutination tests for differentiation between coagulase positive and coagulase negative staphylococci. Acta path, microbiol. scand. Sect. B 1987, 95, 83–84.

    CAS  Google Scholar 

  • Gronvall G, Holmberg O, Ripa T: Protein A in Staphylococcus aureus strains of human and bovine origin. Acta pathol. microbiol. scand. Sect. B 1972, 80, 735–742.

    Google Scholar 

  • Jeljaszevicz J, Switalski LM, Adlam C: Staphylococci and staphylococcal infections, Vol. 2. In: Easmon CSF, Adlam C (Eds.). Academic Press, London 1983, 525–557.

  • Kloos WE, Schleifer KH: Genus IV. Staphylococcus. In: Holt JG (Ed.): Bergeys Manual of Systematic Bacteriology vol 2. Williams & Wilkins, Baltimore 1986, 1013–1035.

  • Niskanen A: Staphylococcal enterotoxins and food poisoning. Production, properties and detection of enterotoxins. Technical Research Centre of Finland, Material and Processing Technology, Publication no. 19, 1977, 83 p.

  • Ruane PJ, Morgan MA, Citron DM, Mulligan ME: Failure of rapid agglutination methods to detect Oxacillin-resistant Staphylococcus aureus. J. clin. Microbiol. 1986, 24, 490–492.

    Article  CAS  Google Scholar 

  • Winblad S, Ericson C: Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Acta pathol. microbial, scand. Sect. B 1973, 81, 150–156.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Department of Food and Environmental Hygiene, Helsinki, Technical Research Centre of Finland, Food Research Laboratory, Espoo, and Department of Biochemistry and Department of Bacteriology and Immunology, University of Helsinki, P. O. Box 6, SF-00581, Helsinki, Finland

    A. Niskanen, H. Korkeala, M. Manninen, M. Vuento & P. Kuusela

Authors
  1. A. Niskanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Korkeala
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Manninen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Vuento
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Kuusela
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Niskanen, A., Korkeala, H., Manninen, M. et al. Evaluation of Three Slide Agglutination Tests for Rapid Identification of Staphylococcus aureus. Acta Vet Scand 32, 543–549 (1991). https://doi.org/10.1186/BF03546956

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03546956

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147