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Comparison of Three Commercial Rapid Agglutination Test Kits for Identification of Coagulase Positive Staphylococci from Foods and Animals
Sammenligning av tre kommersielle agglutina-sjons hurtigtester til identifisering av koagulase-positive stafylokokker fra næringsmidler og dyr
Acta Veterinaria Scandinavica volume 32, pages 155–161 (1991)
Abstract
Three rapid agglutination assays for the identification of Staphylococcus aureus Monostaph (Bionor A/S, Skien, Norway), Staphyslide-Test (BioMerieux, Lyon, France) and Staph-Rapid-Test (Roche, Basel, Switzerland), were compared. A total of 104 Gram-positive, catalase positive cocci were tested: Nineteen Staphylococcus reference strains comprising 15 spp. (4 strains were coagulase positive), and 7 Micrococcus reference strains comprising 4 spp.; 22 food isolates comprising 13 S. aureus, 8 coagulase positive Staphylococcus spp., and 1 Micrococcus sp.; 56 animal isolates comprising 11 S. aureus, 9 S. hyicus subsp. hyicus, 2 S. intermedius, 15 coagulase positive and 19 coagulase negative Staphylococcus spp. Totally 54 strains were coagulase positive.
Considering agglutination of a coagulase positive strain as a correct identification, Monostaph, Staph-Rapid-Test, and Staphyslide-Test correctly identified 52 (96.3 %), 47 (87.0 %) and 48 (89.0 %) of the coagulase positive staphylococci, respectively. Monostaph, Staph-Rapid-Test and Staphyslide-Test showed 1 (2.0%), 4 (8.0%) and 4 (8.0%) false positive reactions respectively. Monostaph, Staph-Rapid-Test and Staphyslide-Test gave 0 (0.0%), 6 (5.8%) and 7 (6.7%) non-interpretable reactions, respectively.
Monostaph may be a good alternative to the tube-coagulase test for rapid and reliable identification of coagulase positive staphylococci from both food and veterinary sources. However, false negative reactions may occur with coagulase positive strains of S. hyicus subsp. hyicus and S. intermedius.
Sammendrag
Tre kommersielt tilgjengelige agglutinasjonstester til identifisering af S. aureus Monostaph (Bionor A/S, Skien, Norge), Staphyslide-test (BioMerieux, Lyon, Frankrike) og Staph-Rapid-Test (Roche, Basel, Sveits) ble sammenlignet. Materialet bestod av 104 Gram-positive, katalase positive kokker. Av referansestammene var 9 Staphylococcus representert med 15 arter mens 7 var Micrococcus med 4 arter. Toogtyve stammer var isolert fra næringsmidler. Av disse var 13 S. aureus, 8 koagulase positive Staphylococcus spp. og en Micrococcus sp. Av de 56 isolatene fra dyr var 11 S. aureus, 9 S. hyicus subsp. hyicus, 2 S. intermedius, 15 koagulase positive og 19 koagulase negative Staphylococcus spp.
Idet det forutsettes att agglutinasjon av en koagulase positiv stamme regnes som korrekt identifikasjon, identifiserte Monostaph, Staph-Rapid-Test og Staphyslide-Test korrekt henholdsvis 96 %, 87 % og 89 % av de koagulase positive stammene (n = 54). Monostaph. Staph-Rapid-Test og Staphyslide-test ga henholdsvis 2 %, 8 % og 8 % falske positive resultater (n = 50), mens de ikke tolkbare resultatene for testene (n = 104) var henholdsvis 0 %, 5,8 % og 6,7 %. Monostaph er et godt alternativ til rørkoagulasetesten for en rask og sikker identiflkasjon av koagulase positive stafylokokker fra dyr og mennesker. Det kan imidlertid forekomme falske negative reaksjoner med koagulase positive S. hyicus subsp. hyicus og S. intermedius stammer, samt kliniske isolater av S. aureus ved primær isolasjon.
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Holme, IJ.R., Rosef, O. & Ewald, S. Comparison of Three Commercial Rapid Agglutination Test Kits for Identification of Coagulase Positive Staphylococci from Foods and Animals. Acta Vet Scand 32, 155–161 (1991). https://doi.org/10.1186/BF03546975
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DOI: https://doi.org/10.1186/BF03546975