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A Comparison between Immunofluorescence staining on Smears from Membrana nictitans (M3 Test), Immunohistopathology and Routine Pathology in Cats with Suspected Feline Infectious Peritonitis (FIP)

En jämförelse av immunofluorescensfärgade utstryk från Membrana nictitans (MS test), immunohistopatologi och rutinpatologi på katter med misstänkt felin infektiös peritonit (FIP)

Abstract

Hök, K: A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP). Acta vet. scand. 1991, 32, 171–176. – An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO)), and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP).

A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80 %), when compared to IFO (using samples from an average of 5 organs per animal). In 21 % of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.

Sammanfattning

Katter, med FIP-sjukdom som misstänkt dödsorsak, undersöktes med användande av 3 metoder: utstryk från membrana nictitans infargad for indirekt immunofluorescence (M3 test) för att påvisa kattcoronavirus- (FCV-) infektion, immunohistopatologi utford på i genomsnitt 5 immunofluorescensfärgade organsnitt (IFO) och rutinpatologi (RP). De 2 immunofluorescensbaserade metoderna stämde väl överens, specificitet och sensitivitet cirka 80 %, trots att M3 testen är baserad på endast ett organ, medan endast ett positivt organ av i genomsnitt 5 undersökta krävdes för att ge ett positivt IFO-svar. Rutinpatologin bekräftade diagnosen i 21 % av fallen. M3 testen som är lätt att utföra, medför minskat arbete, samtidigt som den ger värdefull information vid klinisk, epidemiologisk och patologisk undersökning. Dessutom erhålls en säkrare diagnos genom att påvisa virus istället för antikroppar.

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Correspondence to Kristina Höh.

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Höh, K. A Comparison between Immunofluorescence staining on Smears from Membrana nictitans (M3 Test), Immunohistopathology and Routine Pathology in Cats with Suspected Feline Infectious Peritonitis (FIP). Acta Vet Scand 32, 171–176 (1991). https://doi.org/10.1186/BF03546977

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Keywords

  • Membrana nictitans
  • feline corona virus
  • feline infectious peritonitis
  • FIP
  • indirect immunofluorescence assay