- Published:
A Comparison between Immunofluorescence staining on Smears from Membrana nictitans (M3 Test), Immunohistopathology and Routine Pathology in Cats with Suspected Feline Infectious Peritonitis (FIP)
En jämförelse av immunofluorescensfärgade utstryk från Membrana nictitans (MS test), immunohistopatologi och rutinpatologi på katter med misstänkt felin infektiös peritonit (FIP)
Acta Veterinaria Scandinavica volume 32, pages 171–176 (1991)
Abstract
An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO)), and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP).
A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80 %), when compared to IFO (using samples from an average of 5 organs per animal). In 21 % of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.
Sammanfattning
Katter, med FIP-sjukdom som misstänkt dödsorsak, undersöktes med användande av 3 metoder: utstryk från membrana nictitans infargad for indirekt immunofluorescence (M3 test) för att påvisa kattcoronavirus- (FCV-) infektion, immunohistopatologi utford på i genomsnitt 5 immunofluorescensfärgade organsnitt (IFO) och rutinpatologi (RP). De 2 immunofluorescensbaserade metoderna stämde väl överens, specificitet och sensitivitet cirka 80 %, trots att M3 testen är baserad på endast ett organ, medan endast ett positivt organ av i genomsnitt 5 undersökta krävdes för att ge ett positivt IFO-svar. Rutinpatologin bekräftade diagnosen i 21 % av fallen. M3 testen som är lätt att utföra, medför minskat arbete, samtidigt som den ger värdefull information vid klinisk, epidemiologisk och patologisk undersökning. Dessutom erhålls en säkrare diagnos genom att påvisa virus istället för antikroppar.
References
Barlough JE: Cats, coronaviruses and coronavirus antibody tests. J. small. Anim. Pract. 1985, 26, 353–362.
Horzinek MC, Osterhaus ADME: Feline infectious peritonitis: A worldwide serosurvey. Amer. J. vet. Res. 1979, 40, 1487–1492.
Hök K: Demonstration of feline infectious peritonitis virus in conjunctival epithelial cells from cats. Acta Path. Microb. Immun. Scand. 1989, 97, 820–824.
Lutz H. Hauser B, Horzinek MC: FELINFO No. 1. Feline infectious peritonitis (FIP) – the present state of knowledge. J. small Anim. Pract. 1986, 27, 108–116.
Pedersen NC, Ward J, Mengeling WL: Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other species. Arch. Virol. 1978, 58, 45–53.
Pedersen NC: Coronavirus diseases. Diseases of the cat. Vol. 1. Ed. J Holtzworth. Philadelphia, W. B. Saunders 1987, 193–214.
Robinson RL, Holzworth J, Gilmore CE: Naturally occurring feline infectious peritonitis. Signs and clinical diagnosis. J. Amer. vet. med. Ass. 1971, 158, 981–986.
Ward JM: Morphogenesis of a virus in cats with experimental feline infectious peritonitis. Virology 1970, 41, 191–194.
Weiss RC, Scott FW: Laboratory diagnosis of feline infectious peritonitis. Feline Pract. 1980, 10, 16–22.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Höh, K. A Comparison between Immunofluorescence staining on Smears from Membrana nictitans (M3 Test), Immunohistopathology and Routine Pathology in Cats with Suspected Feline Infectious Peritonitis (FIP). Acta Vet Scand 32, 171–176 (1991). https://doi.org/10.1186/BF03546977
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03546977