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The Sedimentation Pattern of D-Glucose-6-Phosphate Dehydrogenase from Bovine Fetal and Adult Erythrocytes

Sedimentationsmønstret for D-glucose-6-phosphat dehydrogenase fra bovine føtale og adulte erythrocyter

Acta Veterinaria Scandinavica volume 13, pages 305–318 (1972)Cite this article

Abstract

Erythrocytes from bovine fetuses contain about 2.4 times higher D-glucose-6-phosphate dehydrogenase activities than erythrocytes from adult cows and bulls. Studying whether this is due to the existence of a special fetal type of enzyme or an increased amount of enzyme in fetal erythrocytes, the sedimentation coefficients of the enzymes have been estimated by s-zonal ultracentrifugation, and compared to normal and deficient human erythrocyte D-glucose-6-phosphate dehydrogenase, s-zonal ultracentrifugations have been performed with a computer optimized isokinetic sucrose gradient. The mainlines in the program used for calculation of sedimentation coefficients are described.

Bovine fetal and adult erythrocyte D-glucose-6-phosphate dehydrogenase was found to have the same sedimentation coefficient of 7.4 S which is different from the sedimentation coefficient of 6.4 S of both human types of the enzyme. The sedimentation coefficients of 6-phospho-D-gluconate dehydrogenase from bovine fetal, bovine adult and human erythrocytes were 6 S for all three types of this enzyme.

By cellulose acetate electrophoresis bovine fetal and adult D-glucose-6-phosphate dehydrogenase show the same mobility, again differing from the normal and deficient human type.

The results of these experiments show that bovine fetal and adult erythrocytic D-glucose-6-phosphate dehydrogenase with respects to molecular parameters are closely related and perhaps identical enzymes.

Sammendrag

Kalvefostre har ca. 2,4 gange højere D-glucose-6-phosphat dehydrogenase aktiviteter i erythrocyterne end voksne køer og tyre. I en undersøgelse af, om dette skyldes forekomsten af en speciel føtal type af enzymet eller en forøget mængde, er enzymets sedimentationskoefficient bestemt ved s-zoneultracentrif ugering. Endvidere er det bovine enzym sammenlignet med to typer af humant erythrocyt dehydrogenase, s-zoneultracentrifugeringen er udført med en computeroptimeret isokinetisk sucrose gradient, og sedimentationskoefficienterne er beregnet med et program, hvis principielle opbygning beskrives.

Bovin føtal og adult D-glucose-6-phosphat dehydrogenase fandtes at have samme sedimentationskoefficient på ca. 7,4 S. De bovine typer adskiller sig dermed fra normal og deficient human D-glucose-6-phosphat dehydrogenase, der har en sedimentationskoefficient på 6,4 S. Sedimentationskoefficienten for 6-phospho-D-gluconat dehydrogenase fandtes for bovine føtale og adulte, samt for begge humane typer at være 6 S.

Ved elektroforese på celluloseacetat strips viste bovin føtal og adult D-glucose-6-phosphat dehydrogenase samme mobilitet, der var forskellig fra mobiliteten for begge humane typer af enzymet.

Resultaterne af disse undersøgelser viser, at bovin føtal og adult D-glucose-6-phosphat dehydrogenase med hensyn til molekylære parametre er meget nærtstående, måske identiske enzymer.

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  1. Institute of Medical Biochemistry, University of Aarhus, Denmark

    Jens Steensgaard & Lars Funding

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  1. Jens Steensgaard
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  2. Lars Funding
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This work was supported by grants from Statens Lægevidenskabelige Forskningsråd and Fonden til Laegevidenskabens Fremme.

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Steensgaard, J., Funding, L. The Sedimentation Pattern of D-Glucose-6-Phosphate Dehydrogenase from Bovine Fetal and Adult Erythrocytes. Acta Vet Scand 13, 305–318 (1972). https://doi.org/10.1186/BF03547048

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Acta Veterinaria Scandinavica

ISSN: 1751-0147