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Isolation of Trypanosoma Theileri from the Blood of Two Cows, One Leukotic, One Exhibiting Lymphocytosis

Isolering av Trypanosoma theileri ur blodet från en leukotisk ko, samt från en ko med lymfosytos

Abstract

Trypanosoma theileri was isolated by the blood culture method from a leukotic cow in extremis. The parasite could be maintained along with leukocytes with weekly changes of culture medium up to 6 months. In connection with a subsequent transmission experiment a cow, not inoculated with trypanosomes but kept in the same shelter as the inoculated one, commenced to be positive for T. theileri in its blood cultures. This cow had a long history of lymphocytosis but showed no signs of leukosis when slaughtered. The significance of the findings is discussed from the point of view of false positive diagnosis of bovine leukosis and the possible role of trypanosome in the etiology of leukosis.

Sammanfattning

I blododlingar från en leukotisk ko in extremis påvisades förekomsten av Trypanosoma theileri. Parasiten jämte bovina leukocyter kunde bevaras ända upp till sex månader, med byte av näringslösning varje vecka. I samband med ett senare överföringsexperiment, isolerades Trypanosoma theileri från en annan ko. Denna ko hölls i samma stall, som den trypanosom inokulerade kon, och hade en lång historia av lymfosytos, men visade vid slaktningen inga tecken på leukos. Värdet av dessa observationer har diskuterats med tanke på felaktiga positiva bovin leukos diagnoser, samt trypanosomernas eventuella roll i leukosens etiologi.

References

  • Burkitt, D. P.: A lymphoma syndrome in tropical Africa. Part III. Epidemiological aspects. In G. W. Richter & M. A. Epstein (ed.) : International Review of Experimental Pathology p. 103. Academic Press, New York and London 1963.

  • Burkitt, D. P.: Etiology of Rurkitt’s lymphoma — an alternative hypothesis to a vectored virus. J. nat. Cancer Inst. 1969, 42, 19–28.

    CAS  PubMed  Google Scholar 

  • Burkitt, D. P.: Rurkitt’s lymphoma and malaria. Int. J. Cancer 1970, 6, 1–9.

    PubMed  Google Scholar 

  • Carmichael, J.: Turning sickness of cattle and Trypanosoma theileri. Parasitology 1939, 31, 489–500.

    Google Scholar 

  • Cross, R. F., D. R. Redman & E. H. Bohl: Trypanosomes associated with bovine lymphocytosis. J. Amer. vet. med. Ass. 1968, 153, 571–575.

    CAS  PubMed  Google Scholar 

  • Cross, R. F., C. K. Smith & D. R. Redman: Observations on Trypanosoma theileri infection in cattle. Canad. J. comp. Med. 1971, 35, 12–17.

    CAS  Google Scholar 

  • Dikmans, G., C. A. Manthel & A. H. Frank: Demonstration of Trypanosoma theileri in the stomach of an aborted bovine fetus. Cornell Vet. 1957, 47, 344–353.

    CAS  PubMed  Google Scholar 

  • Ewing, S. A. & D. L. Carnahan: Occurrence of Trypanosoma theileri in bovine peripheral blood. J. Amer. vet. med. Ass. 1967, 150, 1131–1132.

    CAS  PubMed  Google Scholar 

  • Herbert, I. V.: Trypanosoma theileri, Laveran. Vet. Bull. 1964, 34, 563–570.

    Google Scholar 

  • Killick-Kendrick, R.: The diagnosis of trypanosomiasis in livestock. A review of current technique. Vet. Rull. 1968, 38, 191–197.

    Google Scholar 

  • Knuth, P. & P. Behr: Redeutung der in deutschen Rindern vorkommenden Trypanosomen für die Impfungen gegen Hämoglobinurie. (Significance of the presence of Trypanosomes in German cattle for vaccination against haemoglobinuria). Rerl. tierärztl. Wschr. 1911, 6, 96–97.

    Google Scholar 

  • Lamy, L. & G. Bouley: Observation en France, chez un veau, d’un cas d’infection massive a Trypanosoma theileri Laveran. 1902. (Observation in France of a cow with massive Trypanosoma theileri infection). Rull. Acad. vét. Fr. 1967, 40, 323–324.

    CAS  Google Scholar 

  • Levine, N. D.: Protozoan parasites of domestic animals and of man. Burgess Publishing Company, Minneapolis, Minn., USA 1961.

  • Lundholm, B. D., J. Stirz & B. G. McKerchen: Trypanosoma theileri as a contaminant of tissue origin in cultures of fetal bovine kidney cells in vitro. Virology 1959, 8, 394–396.

    CAS  PubMed  Google Scholar 

  • Malmquist, W. A.: Trypanosomes in leukocyte cultures. Vet. Rec. 1965, 77, 350.

    Google Scholar 

  • Marshak, R. R. & D. A. Abt: Bovine leukemia in experimental leukemia, ed. Marvin A. Rich. Appleton-Century-Crofts, New York 1968.

  • Miller, J. M., L. D. Miller, C. Olson & K. G. Gillette: Virus like particles in phytohemagglutinin stimulated lymphocyte cultures with reference to bovine lymphosarcoma. J. nat. Cancer Inst. 1969, 43, 1297–1305.

    CAS  PubMed  Google Scholar 

  • Olson, H.: Studien über das Auftreten und die Verbreitung der Rinderleukose in Schweden. (Studies of the occurrence and distribution of bovine leukosis in Sweden). Acta vet. scand. 1961, 2, Suppl. 2, 13–46.

    Google Scholar 

  • Ristic, M. & W. Trager: Cultivation at 37 °C of a trypanosome (Trypanosoma theileri) from cows with depressed milk production. J. Protozool. 1958, 5, 146–148.

    Google Scholar 

  • Stewart, A. M.: Burkitt’s lymphoma and Malaria. Lancet 1970, ii, 1031.

  • Wells, E. A., W. H. R. Lumsden & G. J. C. McNeillage: Isolation of trypanosomes of the section Stercoraria from cattle in Nigeria and the United Kingdom. Brit. vet. J. 1968, 124, 382–391.

    CAS  Google Scholar 

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This work was supported by grants from the National Council for Natural Sciences and from Sigrid Juselius Foundation

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Strandström, H., Veijalainen, P., Berger, R. et al. Isolation of Trypanosoma Theileri from the Blood of Two Cows, One Leukotic, One Exhibiting Lymphocytosis. Acta Vet Scand 13, 332–339 (1972). https://doi.org/10.1186/BF03547051

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