- Published:
Isolation and Identification of Hepatitis Contagiosa Canis Virus (Hcc Virus) from an Enzootic Outbreak in a Dog Kennel
Isolasjon og identifikasjon av Hepatitis contagiosa canis-virus (HCC-virus) fra et enzootisk utbrudd i en kennel
Acta Veterinaria Scandinavica volume 13, pages 372–380 (1972)
Abstract
Using a continuous cell line of canine kidney (MDCK-cell line) cells, HCC virus was isolated from 4 of 6 puppies affected in an outbreak of the disease in a dog kennel. The virus was most easily, and rapidly, isolated in primary cultures from the liver samples, but also from other organs such as the spleen and lung, although isolation from these organs could require 1 or 2 passages in addition to the primary culture. Attention is therefore drawn to the necessity of using a sufficient number of passages when organs other than the liver are available. Among the bacteria identified in addition to the virus infection, were Staphylococcus aureus, Bordetella bronchiseptica and β-hemolytic streptococci. Emphasis is made on the significance of isolation and identification of the virus in pure culture, thus enabling its various properties to be studied in detail, and possible new serotypes to be recognized.
Sammendrag
Med bruk av en cellelinje av hundenyreceller (MDCK-celler) ble HCC-virus isolert fra 4 av 6 hvalper under et enzootisk utbrudd i en kennel. Viruset ble isolert i primærkultur fra lever, men også fra andre organer som milt og lunge, selv om isolasjon fra sistnevnte organer ofte krevet 1 til 2 passasjer i tillegg til primærkultur. En fremhever derfor betydningen av et tilstrekkelig antall passasjer i cellekultur når andre organer enn lever anvendes for undersøkelse. Som sekundær bakterieinfeksjon forekom Staphylococcus aureus, Bordetella bronchiseptica og β-hemolytiske streptokokker. En påpeker betydningen av isolasjon av viruset i renkultur for at dets forskjellige egenskaper kan studeres i detalj og eventuelle nye serotyper påvises.
References
Andrewes, C. & H. G. Pereira: Viruses of Vertebrates. 2nd Ed. Bailliere, Tindall & Cassell, London 1967.
Bruner, D. W. & J. H. Gillespie: Hagan’s Infectious Diseases of Domestic Animals. 5th Ed. Cornell University Press, Ithaca, New York 1966.
Cabasso, V. J., M. R. Stebbins, T. W. Norton & H. R. Cox: Propagation of infectious canine hepatitis virus in tissue culture. Proc. Soc. exp. Biol. 1954, 85, 239–245.
Fieldsteel, A. H. & G. M. Yoshihara: Propagation of infectious canine hepatitis virus in cultures of pig and ferret kidney. Proc. Soc. exp. Biol. 1957, 95, 683–686.
Gaush, C. R., W. L. Hard & T. F. Smith: Characterization of an established line of canine kidney cells (MDCK). Proc. Soc. exp. Biol. 1966, 122, 931–939.
Green, R. G. & J. E. Shillinger: Epizootic fox encephalitis VI. A description of the experimental infection in dogs. Amer. J. Hyg. 1934, 19, 362–391.
Lehnert, E.: Der Wert der Komplementbindungsmethode bei der Hepatitis contagiosa canis. (The value of the complement fixation test in Hepatitis contagiosa canis). Skand. Vet. T. 1948, 38, 94–107.
Mansi, W.: The value of the complement-fixation test in the study of canine distemper complex and Rubarth’s disease. J. comp. Path. 1955, 65, 291–308.
Mansi, W.: The study of some viruses by the plate gel diffusion precipitin test. J. comp. Path. 1957, 67, 293–303.
Möller, J. & Aa. Thordal-Christensen: Cultivation of the virus of infectious canine hepatitis (Rubarth) in tissue culture. Nord. Vet.-Med. 1954, 6, 767–779.
Rubarth, S.: An acute virus disease with liver lesions in dogs. (Hepatitis contagiosa canis). A pathologico-anatomical and etiological investigation. Acta path, microbiol. scand. 1947, Suppl. 69.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saxegaard, F. Isolation and Identification of Hepatitis Contagiosa Canis Virus (Hcc Virus) from an Enzootic Outbreak in a Dog Kennel. Acta Vet Scand 13, 372–380 (1972). https://doi.org/10.1186/BF03547054
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03547054