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Studies on in Vitro Erythrocyte Uptake of I131-Labelled 1-Triiodothyronine as A Test of Thyroid Function in Dairy Cattle

Untersuchungen über die Aufnahme von J-131-gemerkten l-Trijodthyronins in Erythrocyten in vitro als Test der Thyreoideafunktion bei Rindern

Undersökningar över erythrocyter nas upptag in vitro av J131-märkt Trijodthyronin som en test på thyreoideafunktionen hos nötkreatur

Summary

Uptake of I131-labelled triiodothyronine by erythrocytes (EU test) as a test of thyroid function has been studied in cattle. The normal EU value for 141 Swedish Red-and-white cows was 6.99 ± 0.95 (range 4.45–10.23) at an incubation time of one hour. Statistically significantly higher values were obtained for newly-calved cows. The EU values for calves are significantly higher but by 12 to 18 months of age decline to the same level as for older cows.

The principle of the test is described and the results obtained compared with those for human beings. The EU test is suitable for screening thyroid function for large groups of animals and for studying associations between thyroid activity and different production qualities.

Zusammenfassung

Eine Untersuchung über die Aufnahme gemerkten Trijodthyronins (genannt EU-Test, Erythrocyte Uptake) als Test der Thyreoideafunktion bei Rindern wurde vorgenommen. Basiert auf eine Untersuchung von 141 SRB-Kühen erhielt man einen EU-Normalwert von 6,99 ± 0,95 (Grenzwerte 4,45–10,23), wobei doch die Zeit nahe der Kalbung ausgeschlossen wurde, nachdem man statistisch signifikant höhere EU-Werte bei neugekalbten Kühen erhielt. Jungtiere zeigen höhere EU-Werte, die doch mit steigendem Alter sinken, um nach 12–18 Monaten die gleichen, wie die bei älteren Tieren zu sein.

Das Prinzip, worauf der Test baut, wird mit Leitung der Literaturangaben diskutiert. Die erhaltenen Resultate werden mit denen, besonders beim Menschen erhaltenen, verglichen. Die Verfasser schliessen daraus, dass der EU-Test für eine grobe Aussiebung des Thyreoideastatus bei grösserem Tiermaterial, sowie für Untersuchungen des Zusammenhanges zwischen Thyreoideastatus und verschiedenen Produktionseigenschaften sehr anwendbar ist.

Sammanfattning

En undersökning har företagits över erythrocyternas upptag av markt trijodthy ronin (EU-testen) såsom en test på thyreoideafunktionen hos nötkreatur. Baserat på en undersökning av 141 SRB-kor erhölls ett normalvarde på EU av 6,99 ± 0,95 (gränsvärden: 4,45–10,23) varvid dock tiden närmast efter kalvningen uteslutits, eftersom statistiskt signifikant högre EU-värden erhålles hos nykalvade kor. Ungdjur visar högre EU-värden„ vilka sjunker med stigande ålder för att vid 12–18 månaders ålder vara desamma som hos äldre kor.

Den princip varpå testen bygger diskuteras med ledning av litteraturuppgifter. De erhållna resultaten jämföres med motsvarande resultat hos specieilt människa. EU-testen är väl lämpad för screening av thyreoideastatus på ett större djurmaterial liksom vid undersökning av sambandet mellan thyreoideastatus och olika produktionsegenskaper.

References

  1. Christensen, L. K.: Triiodothyronine uptake by erythrocytes. Endocrinology 1960a, 66, 138–140.

    CAS  PubMed  Google Scholar 

  2. Christensen, L. K.: Triiodothyronine uptake by erythrocytes. Acta med. scand. 1960b, 166, 141–146.

    CAS  Article  Google Scholar 

  3. Hamolsky, M. W., Golodetz, A. & Freedberg, A. S.: The plasma proteinthyroid hormone complex in man. III. Further studies on the use of the in vitro red blood cell uptake of I131-l-triiodothyronine as a diagnostic test of thyroid function. J. clin. Endocr. 1959, 19, 103–116.

    CAS  Google Scholar 

  4. Hamolsky, M. W., Stein, M. & Freedberg, A. S.: The thyroid hormoneplasma protein complex in man. II. A new in vitro method for study of uptake of labelled hormonal components by human erythrocytes. J. clin. Endocr. 1957, 17, 33–43.

    CAS  Google Scholar 

  5. Hansard, S. L.: Evaluation of labelled triiodothyronine in vitro for measuring thyroid status of animals. J. Animal Sci. 1962, 21, 382 (Abstr.).

    Google Scholar 

  6. Ingbar, S. H.: The interaction of the thyroid hormones with the proteins of human plasma. Ann. N. Y. Acad. Sci. 1960, 86, 440–453.

    CAS  PubMed  Google Scholar 

  7. Ingbar, S. H. & Freinkel, N.: Regulation of the peripheral metabolism of the thyroid hormones. Recent Progr. Hormone Res. 1960, 16, 353–403.

    CAS  PubMed  Google Scholar 

  8. Lewis, R. C. & Ralston, N. P.: Protein-bound iodine levels in dairy cattle plasma. J. Dairy Sci. 1953, 36, 33–38.

    CAS  Google Scholar 

  9. Long, J. F., Gilmore, L. O., Curtis, G. M. & Rife, D. C.: Protein-bound iodine as related to age, sex and breed. J. Animal Sci. 1951, 10, 1027–1028.

    Google Scholar 

  10. Mitchell, M. L., Harden, A. B. & O’Rourke, M. E.: The in vitro resin sponge uptake of triiodothyronine-I131 from serum in thyroid disease and in pregnancy. J. clin. Endocr. 1960, 20, 1474–1483.

    CAS  PubMed  Google Scholar 

  11. Mixner, J. P., Kramer, D. H. & Szabo, K. T.: Effects of breed, stage of lactation, and season of year on thyroid secretion rate of dairy cows as determined by the chemical thyroxine turnover method. J. Dairy Sci. 1962, 45, 999–1002.

    CAS  Google Scholar 

  12. Monroe, R. A. & Turner, C. W.: Thyroid secretion rate of albino rats during growth, pregnancy and lactation. Res. Bull. Mo. agric. Exp. Sta. 1946, No 403.

  13. Moustgaard, J. & Thorbeck, G.: Undersøgelser over jodkaseinets virkning på mælksekretionen og stofskiftet hos køer. 240. Beretn. Forsøgslab., Copenhagen 1949.

  14. Owen, E. C.: The effects of thyroxine on the metabolism of lactating cows. I. General results and nitrogen metabolism. Biochem. J. 1948, 43, 235–243.

    CAS  Google Scholar 

  15. Parrow, A.: The plasma protein binding of triiodothyronine and its relation to the uptake of I131-labelled triiodothyronine by human erythrocytes in vitro. Scand. J. Clin. Lab. Invest. 1962, 14, 192–202.

    CAS  Google Scholar 

  16. Parrow, A. & Werner, L: Determination of the triodothyronine uptake by erythrocytes — a practical routine test of the thyroid function. Acta med. scand. 1962, 171, 621–631.

    CAS  Google Scholar 

  17. Pipes, G. W., Bauman, T. R., Brooks, J. R., Comfort, J. E. & Turner, C. W.: Effect of season, sex and breed on the thyroxine secretion rate of beef cattle and a comparison with dairy cattle. J. Animal Sci. 1963, 22, 476–480.

    Google Scholar 

  18. Post, T. B. & Mixner, J. P.: Thyroxine turnover methods for determining thyroid secretion rates in dairy cattle. J. Dairy Sci. 1961, 44, 2265–2277.

    Google Scholar 

  19. Premachandra, B. N., Pipes, G. W. & Turner, C. W.: Variation in the thyroxine secretion rate of cattle. J. Dairy Sci. 1958, 41, 1609–1615.

    CAS  Google Scholar 

  20. Robbins, L. R.: Experience with the in vitro erythrocyte uptake of I131-labelled 1-triiodothyronine in a routine clinical laboratory. J. clin. Endocr. 1959, 19, 1292–1301.

    CAS  Google Scholar 

  21. Robbins, J. & Rail, J. E.: Proteins associated with the thyroid hormones. Physiol. Rev. 1960, 40, 415–489.

    CAS  PubMed  Google Scholar 

  22. Schultze, A. B. & Turner, C. W.: The determination of the rate of thyroxine secretion by certain domestic animals. Res. Bull. Mo. agric. Exp. Sta. 1945, No. 392.

  23. Slebodzinski, A.: Uptake of iodothyronines by erythrocytes in vitro in various species. Nature (Lond.) 1963, 199, 75–76.

    CAS  Google Scholar 

  24. Sorensen, P. H.: Jodstoftskifte og thyreoideafunktionen hos kvaeg og svine (Iodine metabolism and thyroid function in cattle and pigs). 302. Beretn. Forsøgslab. Copenhagen 1958.

  25. Thorell, C. B.: To be publ. 1965.

  26. Wilson, R. B., Dickson, W. M. & Frost, F. N.: A procedure for assay of thyroid status in animals. Vet. Med. 1961, 56, 285–289.

    Google Scholar 

  27. Yen, W.: In vitro studies of thyroid function with erythrocyte uptake of 131-I-labelled triiodothyronine. Acta endocr. (Kbh.) 1963, 42, 1–44, Suppl. 76.

    Google Scholar 

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Correspondence to Lars Ekman.

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The investigation was aided by grants from The Swedish Agricultural Research Council and The Atomic Energy Research Council.

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Ekman, L., Thorell, C. Studies on in Vitro Erythrocyte Uptake of I131-Labelled 1-Triiodothyronine as A Test of Thyroid Function in Dairy Cattle. Acta Vet Scand 6, 30–40 (1965). https://doi.org/10.1186/BF03547064

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