Skip to main content
  • Published:

Passage of I131-Albumin and I125-Gamma Globulin Into the Small Intestine of Calves

Der intestinale Durchtritt von J131-Albumin und J125-Gammaglobulin

Udskillelse af J131-albumin og J125-gammaglobulin til tyndtarmen hos kalve

Summary

Intestinal transfer of I131-albumin and I125-gamma globulin was studied in 5 calves fitted with re-entrant intestinal loops. The passage of the two proteins across the intestinal wall proved highly variable (cf. Table II). However, the transfer of I131-albumin and I125-gamma globulin was of the same order of magnitude, which is taken as evideńce of similar transfer mechanisms of albumin and gamma globulin, in spite of different molecular weights. Instillations of hypertonic magnesium sulphate solution and of intestinal digesta were followed by increased transfer rates. It appears, therefore, that the passage of digesta will influence the transfer of proteins along the gut. It is emphasized that quantitation of the transfer as related to total catabolism of plasma proteins is subject to considerable inaccuracy.

Zusammenfassung

bei Kälbern.

Der intestinale Durchtritt von J131-Albumin und J125-Gammaglobulin bei 5 Kälbern mit isolierten Darmsegmenten wurde untersucht. Ausserordentliche Variation wurde festgestellt (Tabelle II), wogegen eine Vergleichung zwischen dem Durchtritt von Albumin und Gammaglobulin erwies, dass dieser von gleicher Grösse war. Dies wird als Schein daran gehalten, dass der Durchtritt von beiden Eiweissstoffen dem selben metabolischen Muster folgt, trotz verschiedenes Molekülgewicht. Instillationen von hypertonischen Magnesiumsulfatlösungen und von Ingesta rufen eine Steigerung im Durchtritt hervor. Es ist deshalb sehr wahrscheinlich, dass die Darmpassage für den Durchtritt von Plasmaproteinen dem Darm entlang eine Rolle spielt. Es wird betönt, dass die quantitative Berechnung des Durchtrittes in Relation zum totalen Eiweiss-Katabolismus unsicher ist.

Sammanfattning

Den intestinale udskillelse af J131-albumin og J125-gammaglobulin er undersøgt hos 5 kalve forsynede med isolerede tarmslynger. Der fandtes overordentlig stor variation i udskillelsen (tabel II), hvorimod en sammenligning mellem udskillelsen af albumin og gammaglobulin viste, at denne var af samme størrelsesorden. Dette er taget som udtryk for, at udskillelsen af de to proteinstoffer følger samme metaboliske mønster trods den forskellige molekylvægt. Instillation af hypertoniske magnesiumsulfatopløsninger samt af tyndtarmsindhold efterfulgtes af en forøget udskillelse af begge plasmaproteiner. Det må derfor anses for sandsynligt, at tarmpassagen spiller en rolle for udskillelsen af serumproteiner ned gennem tarmen. Det understreges, at beregninger af udskillelsens betydning i forhold til proteinnedbrydningen som helhed, er underkastet betydelig usikkerhed.

References

  • Andersen, S. B., J. Glenert & K. Wallevik: Turnover and intestinal degradation of gamma globulin in the dog. I: Peeters, H.: Protides of the biological fluids. Proc. 11th colloquium, Brügge 1963. Elsevier Publishing Co., Amsterdam 1964, p. 272.

  • Armstrong, F. B., S. Margen & H. Tarver: Plasma protein VII. Site of degradation of serum albumin. Proc. Soc. exp. Biol. (N. Y.) 1960, 103, 592.

    Article  CAS  Google Scholar 

  • Ash, R. W.: Gastrointestinal re-entrant cannulae for studies of digestion in sheep. Anim. Prod. 1962, 4, 309.

    Google Scholar 

  • Barandun, S., D. Nusslé, H. P. Witschi & F. Buser: Untersuchungen über den Durchtritt von Plasmaproteinen in das Darmlumen bei gesunden Kindern. Schweiz, med. Wschr. 1962, 92, 316.

    CAS  Google Scholar 

  • Birke, G., R. Gullberg,, S.-O. Liljedahl, B. Olhagen, L.-O. Plantin & J. Wetterfors: Studier av albumin läkage i ventrikelen. Nord. Med. 1960, 64, 1179.

    Google Scholar 

  • Birke, G., S.-O. Liljedahl, B. Olhagen, L.-O. Plantin & S. Ahlinder: Catabolism and distribution of gamma globulin. Acta med. scand. 1963, 173, 589.

    Article  CAS  Google Scholar 

  • Campbell, R. M., D. P. Cuthbertson, W. Mackie, A. S. McFarlane, A. T. Phillipson & S. Sudsaneh: Passage of plasma albumin into the intestine of the sheep. J. Physiol. 1961, 158, 113.

    CAS  PubMed  Google Scholar 

  • Dich, J. & K. Nielsen: Passage of I131-albumin and I125-gamma globulin into the small intestine of the pig. Can. J. comp. Med. vet. Sci. 1964, 28, 257.

    CAS  Google Scholar 

  • Franks, J. J., E. L. Mosser & G. L. Anstadt: The role of the gut in albumin catabolism. I. Studies in the jejunoileectomized rabbit. J. gen. Physiol. 1963 a, 46, 415.

  • Franks, J. J., K. W. Edwards, W. W. Lackey & J. B. Fitzgerald: The role of the gut in albumin catabolism. II. Studies in enterectomized rabbits. J. gen. Physiol. 1963 b, 46, 427.

  • Glenert, J., S. Jarnum & S. Riemer: The albumin transfer from blood to gastrointestinal tract in dogs. Acta chir. scand. 1962, 124, 63.

    CAS  PubMed  Google Scholar 

  • Gordon, R. S., jr.: Exudative enteropathy. Abnormal permeability of the gastrointestinal tract, demonstrable with labelled polyvinylpyrrolidone. Lancet 1959, 1, 325.

    PubMed  Google Scholar 

  • Hogan, J. P. & A. T. Phillipson: The rate of flow of digesta and their removal along the digestive tract of the sheep. Brit. J. Nutr. 1960, 14, 147.

    CAS  PubMed  Google Scholar 

  • Holman, H., W. F. Nickel, jr., & M. H. Sleisinger: Hypoproteinemia antedating intestinal lesions and possibly due to excessive serum protein loss into the intestine. Amer. J. Med. 1959, 27, 963.

    CAS  PubMed  Google Scholar 

  • Matthews, C. M. E.: The theory of tracer experiments with I131-labelled plasma proteins. Phys. in Med. Biol. 1957, 2, 36.

    CAS  Google Scholar 

  • McFarlane, A. S.: Efficient trace-labelling of proteins with iodine. Nature (Lond.) 1958, 182, 53.

    CAS  Google Scholar 

  • Nasset, E. S., P. Schwartz & H. V. Weiss: Digestion of proteins in vivo. J. Nutr. 1955, 56, 83.

    CAS  PubMed  Google Scholar 

  • Schwartz, M. & S. Jarnum: Gastrointestinal protein loss in idiopathic (hypercatabolic) hypoproteinemia. Lancet 1959, 1, 327.

    CAS  PubMed  Google Scholar 

  • Tarver, H., F. B. Armstrong, J. R. Debro & S. Margen: Catabolism of plasma protein in the gut. Ann. N. Y. Acad. Sci. 1961, 94, 23.

  • Ullberg, S., G. Birke, B. Ewaldson, E. Hansson,, S.-O. Liljedahl, L.-O. Plantin & J. Wetterfors: The role of the gastrointestinal tract in the elimination of serum albumin. Acta med. scand. 1960, 167, 421.

    CAS  PubMed  Google Scholar 

  • Wetterfors, J., R. Gullberg, S.-O. Liljedahl, L.-O. Plantin, G. Birke & B. Olhagen: Role of stomach and small intestine in albumin breakdown. Acta med. scand. 1960, 168, 347.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This investigation was aided by a grant from Statens almindelige Videnskabsfond

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nielsen, K., Dich, J. Passage of I131-Albumin and I125-Gamma Globulin Into the Small Intestine of Calves. Acta Vet Scand 6, 249–260 (1965). https://doi.org/10.1186/BF03547082

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547082