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Distribution of Selenium in Mice Studied by Whole-Body Autoradiography After Injection of Se75-Sodium Selenite

Die Verteilung von Selenium bei Mäusen auf Grund des Studiums mittels Autoradiographie des ganzen Körpers nach der Injektion von Se75-Natriumselenit

Distribution av selen hos mus studerad med helkroppsautoradiografi efter injektion av Se75-natriumselenit

Summary

The distribution in the mouse of Se75 after injection of Se75 sodium selenite was studied by autoradiography of sagittal whole-body sections. The animals were killed at various times, from 5 minutes to 8 days, after a single intravenous injection.

The distribution was characterized by a high blood level of selenium during the whole observation period.

The highest concentration of selenium soon after the injection was found in the liver, heart, kidney and adrenal cortex. Eight days after the injection the highest concentration was seen in the liver and the kidney. The selenium penetrated the placenta, but the uptake was much lower in the fetuses than in the maternal tissue.

Zusammenfassung

Die Verteilung von Se75 nach der Injektion von Se75-Natriumselenit wurde bei der Maus mittels Autoradiographie sagittaler Körperschnitte studiert. Die Tiere wurden zu verschiedenen Zeitpunkten, von 5 Minuten bis zu 8 Tagen, nach der intravenösen Injektion getötet.

Die Verteilung kennzeichnete sich durch hohen Selengehalt im Blut während der ganzen Beobachtungszeit.

Die höchste Selenkonzentration fand sich gleich nach der Injektion in der Leber, im Herzen, in den Nieren und der Nebennierenrinde. Nach acht Tagen war der Selengehalt in der Leber und Niere am höchsten. Das Selen passierte die Plazenta, die Konzentration war jedoch in den Geweben des Fötus bedeutend niedriger als in denjenigen der Mutter.

Sammanfattning

Distributionen av Se75 efter injektion av Se75-natriumselenit studerades hos mus med autoradiografi av sagittala kroppssnitt. Djuren avlivades vid olika tidpunkter, från 5 minuter till 8 dygn, efter den intravenösa injektionen.

Distributionen karakteriserades av hög selenhalt i blodet under hela observationstiden.

Högsta koncentrationen selen fanns strax efter injektionen i lever, hjärta, njurar och binjurebark. Efter 8 dygn var halten högst i lever och njure. Selenet passerade placentan, men koncentrationen var avsevärt lägre i fostrets an i moderns vävnad.

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Correspondence to S. O. Jacobsson.

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This investigation was supported by grants from the Swedish Medical Research Council and the Swedish Agricultural Research Council.

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Jacobsson, S., Hansson, E. Distribution of Selenium in Mice Studied by Whole-Body Autoradiography After Injection of Se75-Sodium Selenite. Acta Vet Scand 6, 287–298 (1965). https://doi.org/10.1186/BF03547086

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