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Thermostability of Distemper Virus and Its Neutralization by Antibody

Der Hundestaupe Virus: seine Wärmestabilität und die Neutralisierung von den Antikörpern

Valpsjukevirus: dess varmestabilitet och neutralisering medelst antikroppar

Acta Veterinaria Scandinavica volume 6, pages 329–340 (1965)Cite this article

Summary

The thermostability of distemper virus in a phosphate-glycerine buffer of pH 7.4 was studied at various temperatures.

Thermal inactivation curves suggested first order kinetics except at 25° C where the curves exhibited initial shoulders. The estimated half-life for distemper virus at 5, 25, 37 and 56° C were 3–4 days, 10 hours, 1 hour and % minute respectively. A single straight line relationship could not be demonstrated in the Arrhenius plot.

Approximate equilibrium for the reaction between distemper virus and small amounts of neutralizing antibody was established at 5°C in 2 to 3 days and at 25° C in 16–20 hours. It was concluded that 16 hours at 25° C provided an adequate and convenient incubation for the virus antibody reaction in a sensitive test for distemper virus neutralizing antibody.

Zusammenfassung

Die Wärmestabilität des Hundestaupe Virus war in einem Phosphat-Gyzerin Puffer von pH 7,4 bei verschiedenen Temperaturen studiert worden.

Die Kurven der Wärmeinaktivierung erschienen bei erstem Grade Kinetik, ausser bei 25 °C, wo die Anfangsinaktivierung von dem ersten Grade Reaktion abweichte Die Halbierungszeiten für den Hundestaupe Virus bei 5, 25, 37 und 56°C waren auf 3–4 Tage, 10 Stunden, 1 Stunde und ½ Minute geschätzt worden. Einen einfachen, geradlinigen Zusammenhang konnte man in dem Arrhenius „plot” nicht bekommen.

Die Reaktion zwischen dem Hundestaupe Virus und geringen Mengen neutralisierender Antikörper, erreichte ungefähr das Gleichgewicht bei 5°C in 2 bis 3 Tagen, und bei 25°C in 16 bis 20 Stunden.

Eine empfindliche Untersuchung, neutralisierender Antikörper gegen Hundestaupe Virus, gewährte eine Inkubationszeit von 16 Stunden bei 25 °C.

Sammanfattning

Värmestabiliteten hos valpsjukevirus studerades vid olika temperaturer i en fosfat-glycerin buffert av pH 7.4.

Värme-inaktiveringskurvorna förehöll uppvisa första graden kinetik utom vid 25°C dar den initiala inaktiveringen avvek från en första grads reaktion. Halveringstiderna för valpsjukevirus vid 5, 25, 37 och 56°C uppskattades till 3–4 dagar, 10 timmar, 1 timme och ½ minut respektive. Ett enkelt rätlinjigt samband erhölls ej i Arrhenius „plot”.

Reaktionen mellan valpsjukevirus och små mängder neutraliserande antikroppar uppnådde ungefärlig jämvikt vid 5°C inom 2 till 3 dagar och vid 25°C inom 16 till 20 timmar. 16 timmar vid 25°C erbjöd ett adekvat och lämpligt inkuberingsförfarande för virus-antikropps reaktionen i en känslig test för neutraliserande antikroppar.

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Authors and Affiliations

  1. Department of Virus Research, Karolinska Institutet Medical School, Stockholm, Sweden

    J. P. Lo & S. E. Svehag

  2. Animal Disease Research Laboratory, Animal Disease and Parasite Research Division, Agricultural Research Service, U.S. De¬partment of Agriculture and Washington Agricultural Experiment Station, Washington State University, Pullman, Washington, USA

    J. R. Gorham

Authors
  1. J. P. Lo
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  2. S. E. Svehag
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  3. J. R. Gorham
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Additional information

Abstract of portion of author’s Master of Science thesis, Washington State University, 1963.

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Lo, J., Svehag, S.E. & Gorham, J. Thermostability of Distemper Virus and Its Neutralization by Antibody. Acta Vet Scand 6, 329–340 (1965). https://doi.org/10.1186/BF03547090

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Acta Veterinaria Scandinavica

ISSN: 1751-0147