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Studies on the Dna Content, Dry Mass and Optical Area of Morphologically Normal and Abnormal Bull Spermatozoal Heads

Untersuchungen über DNA-Gehalt, Trockengewicht und Fläche morphologisch normaler und anomaler Spermatozoenköpfe von Bullen

Undersökningar över DNA-mängd, torrvikt och cellyta av morfologiskt normala och abnorma spermiehuvuden hos tjur

Acta Veterinaria Scandinavica volume 7, pages 1–20 (1966)Cite this article

Summary

Quantitative microspectrophotometric, microinterferometric and microplanimetric techniques were used to compare morphologically normal and abnormal bull spermatozoal heads with respect to DNA content, total dry mass and optical area. Spermatozoa were obtained from ejaculates of two young bulls with disturbances in spermatogenesis. U. v. microspectrophotometric results exhibited no difference in mean amounts of DNA between normal and abnormal spermatozoal heads. Apparently contradictory results were found with Feulgen microspectrophotometry and were discussed. Highly significant differences in mean values were observed between the normal and abnormal heads for total dry mass and optical area. The only cytophotometric parameter in which a significant difference existed between the variances of the two morphological groups was optical area.

Zusammenfassung

Es wurden quantitative mikrospektrophotometrische, mikrointerferometrische und mikroplanimetrische Methoden angewandt, um morphologisch normale und anomale Spermatozoenköpfe von Bullen in Bezug auf DNA-Gehalt, Trockengewicht und Fläche zu vergleichen. Die Spermatozoen wurden von Ejakulaten zwei junger Bullen, die Störungen in der Spermatogenese aufwiesen, erhalten. U. v.-mikrospektrophotometrische Messungen ergaben keinen Unterschied in der durchschnittlichen DNA-Menge normaler und anomaler Spermatozoenköpfe. Dem widersprachen Resultate Feulgen-mikrospektrophotometrischer Messungen. Der Widerspruch wird diskutiert. Es wurden sehr signifikante Unterschiede in den Mittelwerten für Trockengewicht und Totalfläche zwischen normalen und anomalen Spermienköpfen gefunden. Der einzige cytophotochemische Parameter, in dem ein signifikanter Unterschied zwischen den Variationen der beiden morphologischen Gruppen zum Ausdruck kam, war die Fläche.

Sammanfattning

Kvantitativ mikrospektrofotometri, mikrointerferometri och mikroplanimetri användes för att jämföra morfologiskt normala och abnorma spermiehuvuden hos tjur med avseende på DNA-mängd, torrvikt och cellyta. Spermierna erhölls från ejakulat av två unga tjurar med rubbning i Spermatogenesen. U. v. mikrospektrofotometri visade ingen skillnad i medelvärdena för DNA-mängd mellan normala och abnorma spermiehuvuden. Till synes motsägande resultat erhölls med Feulgen mikrospektrofotometri och dessa diskuteras. Starkt signifikanta skillnader i medelvärden befanns råda mellan de normala och de abnorma spermiehuvudena med avseende på torrvikt och cellyta. Cellytan var den enda parameter i vilken de båda morfologiska grupperna visade signifikant olika varians.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Royal Veterinary College and the Institute for Cell Research, Nobel Medical Institute, Royal Caroline Institute, Stockholm, Sweden

    Barton L. Gledhill

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  1. Barton L. Gledhill
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Additional information

These studies were supported by funds from the Swedish Agricultural Research Council, the Wallenberg Foundation and the Damon Runyon Memorial Fund. The author was the holder of a Public Health Service fellowship (1-F2-GM-14, 733-01A2) from the National Institute of General Medical Sciences, Public Health Service while performing this investigation.

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Gledhill, B.L. Studies on the Dna Content, Dry Mass and Optical Area of Morphologically Normal and Abnormal Bull Spermatozoal Heads. Acta Vet Scand 7, 1–20 (1966). https://doi.org/10.1186/BF03547094

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Acta Veterinaria Scandinavica

ISSN: 1751-0147