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Biochemical Properties of Haemolytic E. Coli Strains Belonging to O-Groups 138, 139 and 141

Biochemische Untersuchungen von hämolytischen E. coli Stämmen den O-Gruppen 138, 139 und 141 zugehörig

Biokemiska undersökningar av hämolyserande E. coli stammar tillhörande O-grupperna 138, 139 och 141

Summary

The biochemical reactions of 171 strains of haemolytic E. coli were examined. Of these strains, 79 had the serological formula O 138 :K 8KB) : (H 14), 6 belonged to the serotype O 139 :K 82(B) :H 1, and 86 to the serotype O 141 :K85(B) : (H4). The O 138 strains, unlike the O 141 strains, were usually sorbose positive, saccharose positive, and salicin negative (see Table 1). As a rule they fermented sugars either promptly or not at all. Several strains (105 of 171) were considered to show urease activity. This reaction is discussed. About 50 per cent of the strains decarboxylated lysine and about 25 per cent could break down arginine although the relative numbers of the strains with these properties were different for the various 0-groups. Twelve indole-negative and 15 KCN-positive strains were encountered.

Zusammenfassung

171 hämolytische E. Coli Stämme sind biochemisch untersucht worden. 79 Stämme hatten die serologische Formel 0138:K81/B/: /H 14/, 6 Stämme O 139 :K 82/B/:H 1 und 86 Stämme 0141:K85/B/: /H 4/. Die O 138-Stämme waren im Gegensatz zu den O 141-Stämmen in der Regel sorbosepositiv, saccharosepositiv und salicinnegativ (siehe Tabelle 1). Die Vergärungen finden meistens prompt nach einem Tage oder garnicht statt. Mehrere Stämme (105 von 171) wurden ureasposiitiv bezeichnet. Die Reaktion wird diskutiert. G:a 50 % der Stämme dekarboxylierten Lysin und c:a 25 % spalteten Arginin. Die Prozentziffern waren verschieden bei verschiedenen O-Gruppen. 12 indolnegative und 15 KGN-positive Stämme kamen vor.

Sammanfattning

171 hämolyserande E. coli stammar undersöktes biokemiskt. Av dessa hade 79 st den serologiska formeln O 138 :K 81(B) : (H 14), 6 st formein O 139 :K 82(B) :H 1 och 86 st formeln O 141: K 85(B) : (H 4). O 138 stammarna voro i motsats till O 141 stammarna i regel sorbospositiva, sackarospositiva och salicinnegativa (se tabeli 1). Som regel skedde förjäsningarna snabbt eller ej alls. Ett flertal stammar — 105 av 171 — betecknades som ureaspositiva. Reaktionen diskuteras. Ca 50 % av stammarna dekarboxylerade lysin och ca 25 % nedbröt arginin. Procentsiffrorna varierade i de olika O-grupperna. 12 indolnegativa och 15 KCN positiva stammar påvisades.

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This work has been supported by a grant from Statens medicinska forskningsråd.

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Månsson, I. Biochemical Properties of Haemolytic E. Coli Strains Belonging to O-Groups 138, 139 and 141. Acta Vet Scand 3, 79–87 (1962). https://doi.org/10.1186/BF03547131

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