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The Growth Factor Requirements of Salmonella Dublin

Wachstums faktoren bei Salmonella dublin

Salmonella dublin’s behov au tillväxtfaktorer

Summary

Nine strains of S. dublin isolated from animals of different species and from different countries were unable to grow in a glucose-mineral salts medium. All strains grew well, however, when nicotinic acid was added to the medium. Intermediate and R strains do not differ qualitatively or quantitatively from S strains. The minimal amount of nicotinic acid necessary for growth was shown to be 0,20 µg. per ml. of medium.

The rates of oxidation of DL-alanine, DL-serine and glucose were essentially the same for all S. dublin variant strains.

Zusammenfassung

Neun von verschiedenen Tierarten in Amerika und Europa isolierte S. dublin Stämme waren nicht in der Lage sich in einer Mineralsalze und Glukose enthaltenden Nährflüssigkeit zu entwickeln. Sie erfordern Nikotinsäure — in einer Mindestmenge von 0.20 µg. per ccm. Nahrungsflüssigkeit. Der Zusatz von 7 anderen Vitaminen konnte diesen Wachstumsfaktor nicht ersetzen. Die Nährungsbedürfnisse von intermediären und R-Stämmen unterschieden sich in dieser Beziehung weder qualitativ noch quantitativ von S-Stämmen. Nikotinsäure ist demnach ein unentbehrlicher Wachstumsfaktor für die untersuchten S. dublin Stämme. In ihrer Oxidationsgeschwindigkeit zeigten die untersuchten S-, I- und R-Varianten von S. dublin keinen signifikanten Unterschied.

Sammanfattning

Nio S. dublin stammar isolerade från olika djurslag i olika länder kunde ej fås att växa i ett substrat innehållande glykos och mineralsalter. Stammarna växte endast när nikotinsyra tillsattes ett sådant substrat. Den minsta mängd nikotinsyra som erfordrades for att erhålla växt visade sig vara 0,20 µg. per ml. substrat. Nikotinsyran kunde ej ersättas genom tillsats av 7 andra vitaminer. Intermediära och R-stammars näringskrav i detta hänseende skiljde sig ej kvalitativt eller kvantitativt från S-stammars. Någon signifikant skillnad i oxidationshastigheten för de undersökta S-, I- och R-varianterna av S. dublin var ej påvisbar.

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Correspondence to H. G. Bayne.

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The work reported here was done during tenure of a fellowship supported by the International Cooperation Administration under the Visiting Research Scientists Program administered by the National Academy of Sciences of the United States of America.

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Bayne, H.G., Thal, E. The Growth Factor Requirements of Salmonella Dublin. Acta Vet Scand 3, 119–125 (1962). https://doi.org/10.1186/BF03547134

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