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Immunological Conditions of Transferrin Types in Cattle

Die immunologischen Verhalten der Transferrintypen bei dem Rindvieh

Transferrintypernes immunologiske forhold hos kvæget

Summary

Electrophoretic separation of cattle plasma in starch gel, with subsequent antigen-antibody reaction in agar gel and absorption experiments have proved that transferrins in cattle are immunologically related. It was also shown that transferrin types in cattle may be demonstrated by Immunoelectrophoresis although an agreement between the two methods was only 75–80 per cent. This discrepancy may be due to poorer electrophoretic separation ability in agar gel and also to the influence of quantitative differences on the location of the precipitates.

Zusammenfassung

Mittels elektrophoretischer Trennung von Rinderplasma in Stärkegel und nachfoldender Antigen- Antikörper Reaktion in Agargel und Absorptions ver suche wird es nachge weist, dass die Transferrinen des Rindviehs immunologisch verwandt sind. Es wird weiterhin gezeigt, dass die Transferrintypen immunoelektrophoretisch nachgeweist werden können, jedoch nur mit 75–80 % Übereinstimmung zwischen den beiden Methoden. Diese Nichtübereinstimmung kan von dem geringeren elektrophoretischen Separations vermögen in Agargel und der Einwirkung quantitativer Unterschiede auf der Lage der Precipitaten herrühren.

Resumé

Med eleiktroforetisik separation af kvægplasma i stivelsegel og påfølgende antigen-antistof reaktion i agar gel og absorptionsforsøg påvises det, at transferrinerne hos kvæget er immunologisk beslægtede. Endvidere vises det, at kvægets transferrintyper kan påvises immunelektroiforetisk, dog kun med en overensstemmelse mellem de to metoder på 75–80 %. Denne uoverensstemmelse kan skyldes den ringere elektrofor etiske separationsevne i agargel og kvantitative forskelles indvirkning på precipitaternes beliggenhed.

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Brummerstedt-Hansen, E. Immunological Conditions of Transferrin Types in Cattle. Acta Vet Scand 3, 245–255 (1962). https://doi.org/10.1186/BF03547143

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