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Immunoelectrophoretic and Immunodiffusion Studies on the Seminal Plasma Proteins in Boars
Undersökningar medelst immunoelektrofores och immunodiffusion av proteiner i spermieplasma från galt
Acta Veterinaria Scandinavica volume 13, pages 449–461 (1972)
Abstract
The proteins of boar seminal plasma have been studied with immunoelectrophoresis and immunodiffusion tests. Four different proteins could be identified with immunoelectrophoresis of which all seemed to be specific for seminal plasma. One of these proteins with an electrophoretic mobility corresponding to gammaglobulin had no identity with either IgG or fibrinogen.
With immunodiffusion tests spur of serum proteins could be detected (albumin, transferrin and IgG).
The spontaneous precipitation of boar seminal plasma in agar gels could not wholly be avoided with alterations of pH and ionic strength in the agar gel or with absorption of seminal plasma with swine red blood cells.
One factor in seminal plasma which probably is of importance for the ability to agglutinate red blood cells was isolated and identified as a protein with an electrophoretic mobility corresponding to alphaglobulin. This protein could not be detected in blood serum.
Sammanfattning
Medelst immunoelektrofores och immunodiffusion har proteinkomponenterna i svinspermieplasman undersökts. Fyra olika distinkta proteiner kunde påvisas med immunoelektrofores, varav samtliga synes vara specifika för spermieplasma. Ett av proteinerna med en mobilitet motsvarande serum-gammaglobulin hade ingen identitet med varken IgG eller fibrinogen.
Medelst immunodiffusion kunde dessutom spår av serumproteiner påvisas ex. albumin, transferrin och IgG.
Förmågan hos svinspermieplasma att spontant précipitera kunde ej helt undvikas varken med ändring av pH och jonstyrka i agargelen eller med absorbering av röda blodkroppar från svin.
En faktor i spermieplasma som troligen är orsaken till dess förmåga att agglutinera röda blodkroppar har isolerats och visades vara ett protein med en mobilitet motsvarande alphaglobulin. Denna faktor kunde ej påvisas i blodserum.
References
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Martinsson, K., Thorén, K. Immunoelectrophoretic and Immunodiffusion Studies on the Seminal Plasma Proteins in Boars. Acta Vet Scand 13, 449–461 (1972). https://doi.org/10.1186/BF03547151
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DOI: https://doi.org/10.1186/BF03547151