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Serological Cross-Reactions Between Different Brucella Species and Yersinia Enterocolitica Immunodiffusion and Immunoelectrophoresis

Serologiska kursreaktioner mellan olika Brucella species och Yersinia enterocolitica

Acta Veterinaria Scandinavica volume 13, pages 472–483 (1972)Cite this article

Abstract

By immunodiffusion and immunoelectrophoresis tests in agarose serological cross-reactions were demonstrated between Yersinia enterocolitica type IX and Brucella strains from four species (Brucella abortus, Brucella melitensis, Brucella suis and Brucella neotomae). No qualitative differences between these strains in their tendencies to cross-react with Yersinia enterocolitica type IX were observed. Brucella canis and Brucella ovis, which have nonsmooth colonial morphology, gave no demonstrable cross-reaction with Yersinia enterocolitica type IX.

The results of absorption tests and qualitative staining reaction of the obtained precipitation lines suggest that the antigenic determinants common to Brucella and Yersinia enterocolitica type IX seemed to be associated with the outer layer and in the lipopolysaccharide complex of the respective bacteria. By immunodiffusion and immunoelectrophoresis it was possible to identify in hyperimmune sera those antibodies that derive from Brucella and Yersinia enterocolitica type IX.

Sammanfattning

Immunodiffusion och immunoelektrofores.

Med hjalp av immunodiffusion och immunoelektrofores i agarose har en serologisk korsreaktion påvisats mellan Yersinia enterocolitica typ IX och Brucella stammar från 4 olika species (Brucella abortus, Brucella melitensis, Brucella suis och Brucella neotomae). Några kvalitativa skillnader för dessa stammar med avseende på deras benägenhet att korsreagera med Yersinia enterocolitica typ IX har inte kunnat påvisas. Brucella canis och Brucella ovis, som är kolonimorfologiskt av R-typ, har icke givet någon påvisbar korsreaktion med Yersinia enterocolitica typ IX.

Resultaten från gjorda absorptionsförsök och kvalitativa färgningsreaktioner av erhållna precipitationslinjer tyder på att de gemensamma antigena determinanterna för Brucella och Yersinia enterocolitica typ IX torde finnas hos respektive bakteriecellers ytskickt och i deras lipopolysackaridkomplex. Immunodiffusion och immunoelektrofores visar en möjlighet att i hyperimmunsera kunna identifiera de antikroppar som härrör från Brucella respektive Yersinia enterocolitica typ IX.

References

  • Ahvonen, P., E. Jansson & K. Aho: Marked cross-agglutination between Brucellae and a subtype of Yersinia enterocolitica. Acta path, microbiol. scand. 1969, 75, 291–295.

    CAS  Google Scholar 

  • Baughn, R. E. & B. A. Freeman: Antigenic structure of Brucella suis spheroplasts. J. Bact. 1966, 92, 1298–1303.

    CAS  PubMed  Google Scholar 

  • Braun, W. & A. Bonesiell: Independent variation of characteristics in Brucella abortus variants and their detection. Amer. J. vet. Res. 1947, 8, 386–390.

    CAS  PubMed  Google Scholar 

  • Clausen, J.: Immunochemical Techniques for the Identification and Estimation of Macromolecules. North-Holland Publishing Company, Amsterdam 1969, 531.

  • Crowle, A. J.: Immunodiffusion. Academic Press, New York and London 1961, 306.

  • Diaz, R., L. M. Jones, D. Leong & J. B. Wilson: Differences between Brucella antigens involved in indirect hemagglutination tests with normal and tanned red blood cells. J. Bact. 1967, 94, 499–505.

  • Diaz, R., L. Jones & J. B. Wilson: Antigenic relationship of the gramnegative organism causing canine abortion to smooth and rough Brucellae. J. Bact. 1968, 95, 618–624.

    CAS  Google Scholar 

  • Diaz, R., R. Lacalle, M. P. Medrano & D. Leong: Immunobiological activities of the endotoxin from Yersinia enterocolitica strain M.Y. 79. Proc. Vth Int. Congr. Infectious Diseases, Vienna 1970, 11–17.

  • Henry, B. S.: Dissociation in the genus Brucella. J. infect. Dis. 1933, 52, 374–402.

    Article  Google Scholar 

  • Huruell, B., P. Ahvonen & E. Thal: Serological cross-reactions between different Brucella species and Yersinia enterocolitica. Proc. 11th Nord. Vet. Congr., Bergen 1970, 282.

  • Hurvell, B., P. Ahvonen & E. Thal: Serological cross-reactions between different Brucella species and Yersinia enterocolitica. Agglutination and complement fixation. Acta vet. scand. 1971, 12, 86–94.

    CAS  PubMed  Google Scholar 

  • Leong, D., R. Diaz, K. Milner, J. Rudbach & J. B. Wilson: Some structural and biological properties of Brucella endotoxin. Infect. Immun. 1970, 1, 174–182.

    CAS  PubMed  Google Scholar 

  • Miller, L. & J. A. Houghton: The micro-Kjeldahl determination of the nitrogen content of amino acids and proteins. J. biol. Chem. 1945, 159, 373–383.

    CAS  Google Scholar 

  • Uriel, J. & P. Grabar: A new technique for direct detection of glycoproteins and polysaccharides after electrophoresis or immunoelectrophoresis in agar gel. Analyt. Biochem. 1961, 2, 80–82.

    CAS  PubMed  Google Scholar 

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  1. National Veterinary Institute, Stockholm, Sweden

    B. Hurvell

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  1. B. Hurvell
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Hurvell, B. Serological Cross-Reactions Between Different Brucella Species and Yersinia Enterocolitica Immunodiffusion and Immunoelectrophoresis. Acta Vet Scand 13, 472–483 (1972). https://doi.org/10.1186/BF03547153

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  • DOI: https://doi.org/10.1186/BF03547153

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Acta Veterinaria Scandinavica

ISSN: 1751-0147