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Kappa-Casein in Milk An Immunochemical Investigation of the ϰ-Casein Content in Milk From Danish Cattle Breeds

Kappa-kasein i mælk. En immunkemisk undersøgelse af ϰ-kaseinindholdet i mælk fra danske kvægracer

Acta Veterinaria Scandinavica volume 13, pages 539–553 (1972)Cite this article

Abstract

The ϰ-casein content in milk from the Danish cattle breeds SDM, RDM and Jersey was investigated by means of immunoquantitation in antibody containing agarose gels. The method, which determines the total ϰ-casein complex, is suitable for routine work. The reproducibility of the method was found to be ± 4.1 %.

The ϰ-casein concentration, expressed as percentage of the total protein content in milk, was significantly lower in milk from SDM (21.5%) and RDM (21.3%) than in Jersey (23.9%). The investigation showed that the ϰ-casein constitutes a greater part of the protein in milk than previously assumed.

The amount of ϰ-casein was positively correlated with the protein content in milk, but there was no relation between the relative ϰ-casein content and the percentage of fat, milk yield and the time after calving.

Sammendrag

ϰ-kaseinindholdet i mælk fra SDM, RDM og Jersey blev bestemt ved kvantitativ immunelektroforese i antistofholdig agarosegel.

Metoden, der bestemmer det totale ϰ-kaseinkompleks, er velegnet til rutinemæssige undersøgelser. Reproducerbarheden var ±4,1 %.

Der blev i procent af total protein fundet signifikant lavere ϰ-kaseinindhold i mælk fra SDM (21,5 %) og RDM (21,3 %) end i mælk fra Jersey (23,9 %). Undersøgelsen sandsynliggør, at ϰ-kaseinet udgør en større del af mælkens totalprotein end tidligere antaget.

ϰ-kaseinmængden var positivt korreleret med mælkens proteinindhold. Derimod var der ingen relation mellem det procentiske ϰ-kaseinindhold og fedtprocenten, ydelsen og tidspunktet i laktationen.

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  1. Department of Physiology, Endocrinology and Blood Grouping, Royal Veterinary and Agricultural University, Copenhagen, Denmark

    Mariann Thymann

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  1. Mariann Thymann
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Thymann, M. Kappa-Casein in Milk An Immunochemical Investigation of the ϰ-Casein Content in Milk From Danish Cattle Breeds. Acta Vet Scand 13, 539–553 (1972). https://doi.org/10.1186/BF03547159

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Acta Veterinaria Scandinavica

ISSN: 1751-0147