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Electrophoretic Studies of Normal Rovine and Guinea-Pig Serum

Elektrophoretische Untersuchungen von normalem Kuh- und Meerschweinchenserum

Elektroforetiske undersøgelser af normalt kvæg- og marsvineserum

Summary

On the basis of a finding of 17 serum protein fractions in normal bovine serum and 15 fractions in normal guinea-pig serum on immunoelectrophoresis, an attempt was made to characterize the fractions in terms of salting-out properties, lipid and glycoprotein content, and esterase activity.

Parallel to the immunoelectrophoretic investigations, bovine and guinea-pig serum was subjected to agar-gel micro-electrophoresis and in one case to paper electrophoresis. Agar-gel micro-electrophoresis revealed 6–7 fractions in bovine and guinea-pig serum. In rare cases it was possible to discern further fractions in the γ area following electrophoresis of bovine serum. By means of agar-gel micro-electrophoresis the protein fractions could be characterized in more detail.

Zusammenfassung

Auf Grund der durch Immunelektrophorese gefundenen 17 Serumproteinfraktionen in normalem Kuhserum sowie der 15 Fraktionen in normalem Meerschweinchenserum, wurde es versucht diese Fraktionen näher zu charakterisieren durch die Bestimmung deren Entsalzungseigenschaften, Lipoprotein- und Glykoproteingehalt sowie durch deren Esteraseaktivität.

Parallel mit den immunelektrophoretisohen Untersuchungen wurden Vergleichsuntersuchungen des Kuh- und Meerschweinchenserums mittels Agargelmikroelektrophorese und in einem Falle mittels Papierelektrophorese gemacht.

Im Kuh- und Meerschweinchenserum zeigt die Agargelmikroelektrophorese 6–7 Fraktionen, in seltenen Fällen konnte man nach der Elektrophorese des Kuhserums im γ-Gebiet mehrere Fraktionen unterscheiden. Mittels die Agargelmikroelektrophorese war es möglich die Proteinfraktionen näher zu charakterisieren.

Sammendrag

På grundlag af fundet af 17 serumproteinfrektioner i normalt kvægserum samt 15 fraktioner i normalt marsvineserum ved immunelektroforese søgtes de fundne fraktioner nærmere karakteriseret ved deres udsaltningsforhold, lipoid- og glycoproteinindhold samt esteraseaktivitet.

Parallelt løbende med de immunelektroforetiske undersøgelser underkastedes kvæg- og marsvineserum agargelmikroelektroforese og i et enkelt tilfælde papirelektroforese. Agargelmikroelektroforese visier 6–7 fraktioner i kvæg- og marsvineserum, i sjældnere tilfælde kunne der i γ-området efter elektroforese af kvægserum skelnes flere fraktioner. Agargelmikroelektroforesen muliggjorde yderligere karakterisation af de fundne proteinfraktioner.

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Jensen, I.K. Electrophoretic Studies of Normal Rovine and Guinea-Pig Serum. Acta Vet Scand 4, 64–84 (1963). https://doi.org/10.1186/BF03547173

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