Skip to main content

Studies on the Virus of Hepatitis Contagiosa Canis (HCC)

III. The Viraemic Phase of Hcc Virus Infection in Guinea Pigs

Studien über das Virus des Hepatitis Contagiosa Canis (HCC)

III. Die Viraemische Phase der HCC-Virus Infektion bei Meerschweinchen

Studier över Hepatitis Contagiosa Canis virus (HCC)

III. Virämiskedet vid HCC virus infektion hos marsvin

Summary

The distribution of virus during the viraemia phase of HCC infection in guinea pigs was studied. It was found that the virus was bound to the mononuclear white blood cells.

Antibody titration revealed that several of the animals with viraemia had HCC antibodies in serum.

By an indirect method it was shown that a virus inhibitor appeared in the blood between 2–3 days after inoculation. Its effect gradually increased as long as virus titrations could be performed with certainty as to mean titre value.

Zusammenfassung

Die Virusverteilung wurde in Hinsicht auf verschiedene Blutfraktionen und Blutzellen untersucht. Es wurde gefunden, dass das Virus an die weissen mononucleären Zellen gebunden war.

Eine Anzahl Tiere mit Viraemie besass gleichzeitig Antikörper gegen das Virus im Serum.

Mit Hilfe eine indirekten Methode konnte gezeigt werden, dass ein Virusinhibitor 2–3 Tage nach der Impfung im Blute auftrat. Diese nahm kontinuierlich zu und dies so lange, als Virustitrierungen mit einiger Sicherheit rücksichtlich Mittel-Titer-Wert durchgeführt werden konnten.

Sammanfattning

Fördelningen av virus under virämiskedet med hänsyn till olika blodfraktioner och blodceller undersöktes. Det befanns att virus var bundet till de vita mononukleära cellerna.

Ett flertal djur med virämi hade samtidigt antikroppar mot virus i sina serum.

Genom indirekt metodik kunde det visas, att en virusinhibitor uppträdde i blodet 2–3 dagar efter ympningen. Dess effekt ökade kontinuerligt och så länge som virustitreringar kunde göras med någorlunda säkerhet med hänsyn till titermedelvärde.

References

  1. 1

    Baker, J. A., M. G. Richards, A. L. Brown & C. G. Rickard: Infectious hepatitis in dogs. Proc. 87th Ann. Meet. Amer. vet. med. Ass. 1950, 242.

  2. 2

    Bindrich, H.: Untersuchungen über den Virusgehalt des Blutes und der Organe bei Hundestaupe. Exp. Vet. Med. 1950, 2, 73.

    Google Scholar 

  3. 3

    Bonnier, G. & O. Tedin: Biologisk variationsanalys, Svemska Bokförlaget, Bonniers 1957.

  4. 4

    Danielsson, H. & F. Lundmark: En ny typ av centrifug (spiral-centrifug). Nord. Med. 1957, 57, 175.

    CAS  PubMed  Google Scholar 

  5. 5

    Fenner, F. & G. M. Woodroofe: The pathogenesis of infectious myxomatosis: The mechanism of infection and the immunological response in the European rabbit (Oryotolagus cuniculus). Brit. J. exp. Path. 1953, 34, 400.

    CAS  PubMed  Google Scholar 

  6. 6

    Fichtelius, K.-E.: A simple method to separate lymphocytes from the blood. Acta Soc. Med. Upsaliensis 1951, 56, 27.

    CAS  Google Scholar 

  7. 7

    Fieldsteel, A. H.: Some aspects of infectious canine hepatitis virus in tissue culture. Amer. J. vet. Res. 1956, 17, 380.

    CAS  PubMed  Google Scholar 

  8. 8

    Fleming, A.: A simple method of removing leucocytes from blood. Brit. J. exp. Path. 1926, 7, 281.

    CAS  Google Scholar 

  9. 9

    Florent, A. & J. Leunen: L’hépatite contagieuse du chien. Son existence en Belgique. Sa Symptomatologie et son diagnostic. Essais de transmission experimentale. Ann. Méd. vét. 1949, 93, 225.

    Google Scholar 

  10. 10

    Hornby, H. E.: The distribution of rinderpest virus in infected blood. J. comp. Path. 1928, 41, 17.

    Article  Google Scholar 

  11. 11

    Horstmann, D. M., R. W. McCallum & A. D. Mascola: Viremia in human poliomyelitis. J. exp. Med. 1954, 99, 355.

    CAS  Article  Google Scholar 

  12. 12

    Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmakol. 1931, 162, 480.

    Article  Google Scholar 

  13. 13

    Lehnert, E.: Der Wert der Komplementbindungsmethode bei der Hepatitis contagiosa canis. Skand. Vet.Tidskr. 1948, 38, 94.

    Google Scholar 

  14. 14

    Liu Ch’ien & D. L. Coffin: Studies on canine distemper infection by means of fluorescein-labelled antibody. I. The pathogenesis, pathology, and diagnosis of the disease in experimentally infected ferrets. Virology 1957, 3, 115.

    Article  Google Scholar 

  15. 15

    MacKerras, J. M., M. J. MacKerras & F. M. Burnet: Experimental studies of Ephemeral Fever in Australian cattle. VII. Distribution of the virus in the blood. Commonwealth Scientific and Industrial Research Organization. Bull. 136. 1940.

  16. 16

    Nicolle, M. et Adil-Bey: Études sur la peste bovine. Ann. Inst. Pasteur 1902, 16, 56.

  17. 17

    Parry, H. B. & N. M. Larin: The natural history of virus hepatitis of dogs (Rubarth’s disease). Vet. Rec. 1951, 63, 833.

    Google Scholar 

  18. 18

    Parry, H. B., N. M. Larin & H. Platt: Studies on the agent of canine virus hepatitis (Rubarth’s disease). II. The pathology and pathogenesis of the experimental disease produced by four strains of virus. J. Hyg. 1951, 49, 482.

    CAS  PubMed  Google Scholar 

  19. 19

    Poppensiek, G. C. & J. A. Baker: Persistence of virus in urine as factor in spread of infectious hepatitis in dogs. Proc. Soc. Exp. Biol. Med. 1951, 77, 279.

    CAS  Article  Google Scholar 

  20. 20

    Rockborn, G.: Viraemia and neutralizing antibodies in experimental distemper in dogs. Arch. ges. Virusforsch. 1957, 7, 168.

  21. 21

    Rubarth, S.: An acute virus disease with liver lesion in dogs (Hepatitis contagiosa canis). A pathologico-anatomical and etiological investigation. Acta path, miorobiol. scand. 1947, 24, Suppl. 69.

  22. 22

    Salenstedt, C. R.: Studies on the virus of Hepatitis Contagiosa Canis (HCC). I. The isolation of HCC-virus from the tonsils of a dog. Arch. ges. Virusforsch. 1958, 8, 123.

    CAS  Article  Google Scholar 

  23. 23

    Salenstedt, C. R.: Studies on the virus of Hepatitis Contagiosa Canis (HCC). II. Susceptibility of guinea pigs to experimental infection with HCC virus. Arch. ges. Virusforsch. 1958, 8, 600.

    Article  Google Scholar 

  24. 24

    Schein, H.: Études sur la peste bovine. Ann. Inst. Pasteur 1917, 31, 571.

    Google Scholar 

  25. 25

    Smith, W.: The distribution of virus and neutralizing antibodies in the blood and pathological exsudates of rabbits infected with vaccinia. Brit. J. exp. Path. 1929, 10, 93.

    Google Scholar 

  26. 26

    Todd, C.: Experiments on the virus of Fowl Plague (I). Brit. J. exp. Path. 1928, 9, 19.

    Google Scholar 

  27. 27

    Todd, C. & R. G. White: Experiments on Cattle Plague, Government Press, Cairo 1914.

  28. 28

    Yoffey, J. M. & E. E. Sullivan: The lymphatic pathway from the nose and pharynx. The dissemination of nasally instilled vaccinia virus. J. exp. Med. 1939, 69, 133.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Salenstedt, C.R. Studies on the Virus of Hepatitis Contagiosa Canis (HCC). Acta Vet Scand 4, 356–370 (1963). https://doi.org/10.1186/BF03547196

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547196