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Studies on the Virus of Hepatitis Contagiosa Canis (HCC)
IV. The Pathogenesis of Hepatitis Contagiosa Canis in Dogs
Studien über das Virus des Hepatitis Contagiosa Canis (HCC)
IV. Die Pathogenese von Hepatitis Contagiosa Canis bei Hunden
Studier över Hepatitis Contagiosa Canis virus (HCC)
IV. Patogenesen vid Hepatitis Contagiosa Canis hos hundar
Acta Veterinaria Scandinavica volume 4, pages 371–383 (1963)
Summary
The pathogenesis of HCC virus infection in the dog has been investigated. Dogs were infected with virus given per os. After multiplication in the tonsils and in the draining lymph nodes virus was carried to the blood via the cervical lymph vessels. Virus was also absorbed to cells in the wall of the small intestine. In those cells as well as in the draining lymph nodes a multiplication took place and virus was then brought to the blood by the lymph in vessels from the mesenteric lymph nodes and via ductus thoracicus. By the blood virus was spread over the whole body and was detected in several organs among them the liver. This was shown 3–5 days after virus was detected in the tonsils. It was also demonstrated that dogs could be infected when the virus was given in gelatine capsules. The tonsils thus were excluded from possibility to absorb virus and the infection probably was initiated by absorbtion of the virus to cells in the small intestine.
Zusammenfassung
Eine Untersuchung in Bezug auf die Pathogenese der HCC beim Hunde wurde unternommen. Die Tiere wurden per os mit Virus infiziert. Nach einer Vermehrung in den Tonsillen und regionalen Lymphknoten gelangte das Virus über die Halslymphgefässe in den Blutkreislauf. Es wurde des weiteren an Zellen der Dünndarmwand absorbiert. In diesen Zellen wie auch in den regionalen Lymphdrüsen fand eine Virusvermehrung statt. Von dort aus gelangte der Infektionsstoff über Lymphgefässe der Mesenteriallymphknoten via ductus thoracicus in den Blutkreislauf. Mit dem Blute wurde das Virus im Gesamtorganismus verteilt und konnte in mehreren Organen, u. a. in der Leber nachgewiesen werden. Dies geschah 3–5 Tage nachdem das Virus in den Tonsillen festgestellt worden war. Es konnte weiterhin gezeigt werden, dass die Hunde auch mittels Virus in Gelatinekapseln per os infiziert werden konnten.
Mittels diese Methode konnte die Möglichkeit einer Absorption von Virus an die Tonsillen ausgeschlossen werden. Daraus ist zu schliessen, dass die Infektion durch das Virus nach dessen Absorption an Zellen der Dünndarmwand initiiert wurde.
Sammanfattning
En undersökning över patogenesen vid HCC hos hund genomfördes. Hundarna infekterades med virus per os. Efter förökning i tonsillerna och i de regionära lymfkörtlarna fördes virus via halslymfkärl till blodet. Virus absorberades även till celler i tunntarmsväggen. I dessa celler ävensom i tarmens regionära lymfkörtlar ägde en virus-förökning rum. Därifrån fördes infektionsämnet med lymfkärl från mesenteriallymfkörtlarna via ductus thoracicus till blodet. Med detta spreds virus över hela organismen och kunde påvisas i flera organ bl. a. i levern. Detta inträffade 3–5 dagar efter det att virus hade upptäckts i tonsillerna. Det kunde också visas att hundarna infekterades även då virus tillfördes per gelatinkapslar. Då så skedde uteslöts möjligheten för absorbtion av virus till tonsillerna. Infektionen bör då ha initierats genom att virus absorberats till celler i tunntarmsväggen.
References
Baker, J. A., M. G. Richards, A. L. Brown and C. G. Rikard: Infectious hepatitis in dogs. Proc. 87th Ann. Meet. Amer. Vet. Med. Ass. 1950, 242.
Barr, J., L. Kjellén and A. Svedmyr: Hospital outbreak of adenovirus type 3 infection. A clinical and virologic study on 38 patients partly involved in a nosocomial outbreak. Acta Paediat. 1958, 47, 365.
Brunner, K. T., M. Scheitlin and H. Stiinzi: Zum serologischen Nachweis der Hepatitis contagiosa canis. Schweiz. Arch. Tierheilk. 1951, 93, 443.
Cabasso, V. J., M. R. Stebbins, T. W. Norton and H. R. Cox: Propagation of infectious canine hepatitis virus in tissue culture. Proc. Soc. Exp. Biol. Med. 1954, 85, 239.
Coffin, D. L.: The pathology of so-called acute tonsillitis of dogs in relation to contagious canine hepatitis (Rubarth). A report of five cases. J. Amer. vet. med. Ass. 1948, 112, 355.
Davies, M. C., M. E. Englert, M. R. Stebbins and V. J. Cabasso: Electron microscopic structure of infectious canine hepatitis (ICH) virus — a canine adenovirus. Virology 1961, 15, 87.
Espmark, J. Å. and C. R. Salenstedt: Haemagglutination-inhibition test for titration of antibodies against Hepatitis contagiosa canis (infectious canine hepatitis). Arch. ges. Virusforsch. 1961, 11, 64.
Fieldsteel, A. H. and J. B. Emery: Cultivation and modification of infectious canine hepatitis. Virus in roller tube cultures of dog kidney. Proc. Soc. Exp. Riol. Med. 1954, 86, 819.
Florent, A. and J. Leunen: L’hepatite contagieuse du chien. Son existence en Relgique. Sa Symptomatologie et son diagnostic. Essais de transmission expérimentale. Ann. Méd. Vét. 1949, 93, 225.
Heller, L. A. and C. R. Salenstedt: One-sided immunologic relation between adenovirus and infectious canine hepatitis antigens. Virology 1960, 11, 640.
Huebner, R. J., W. P. Rome, T. G. Ward, R. H. Parrot and J. A. Bell: Adenoidal-pharyngeal-conjunctival agents. A newly recognized group of common viruses of the respiratory system. New Eng. J. Med. 1954, 251, 1077.
Kapsenberg, J. G.: Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Bıol. Med. 1959, 101, 611.
Kjellén, L., G. Sterner and A. Suedmyr: On the occurrence of adenovirus in Sweden. Acta Paediat. 1957, 46, 164.
Kärber, G.: Reitrag zur kollektiven Rehandlung pharmakologischer Reihenversuche. Arch. exp. Pharmak. 1931, 162, 480.
Larin, N. M.: Studies on the agent of canine virus hepatitis (Rubarth’s disease) I. Complement-fixation and precipitin tests. J. Hyg. 1951, 49, 410.
Lehnert, E.: Der Wert der Komplementbindungsmethode bei der Hepatitis contagiosa canis. Skand. Vet.Tidskr. 1948, 38, 94.
Mansi, W.: Rubarth’s disease (canine virus hepatitis) I. Reaction of dogs to the conjunctival instillation of the virus. J. comp. Path. 1953, 63, 236.
Mansi, W.: The value of the complement-fixation test in the study of canine distemper complex and Rubarth’s disease. J. comp. Path. 1955, 65, 291.
Müller, J. and Aa. Thordal-Christensen: Cultivation of the virus of infectious canine hepatitis (Rubarth) in tissue culture. Nord. Vet.-Med. 1954, 6, 767.
Parry, H. B., N. M. Larin and H. Platt: Studies on the agent of canine virus hepatitis (Rubarth’s disease). II. The pathology and pathogenesis of the experimental disease produced by four strains of virus. J. Hyg. 1951, 49, 482.
Poppensiek, G. C. and J. A. Baker: Persistance of virus in urine as factor in spread of infectious hepatitis in dogs. Proc. Soc. Exp. Biol. Med. 1961, 77, 279.
Rubarth, S.: An acute virus disease with liver lesion in dogs (Hepatitis contagiosa, canis). A pathologico-anatomical and etiological investigation. Acta path, microbiol. scand. 1947, 24, Suppl. 69.
Salenstedt, C. R.: Studies on the virus of Hepatitis Contagiosa Canis (HCC). I. The isolation of HCC-virus from the tonsils of a dog. Arch. ges, Virusforsch. 1958, 8, 123.
Salenstedt, C. R.: Studies on the virus of Hepatitis Contagiosa Canis (HCC). II. Susceptibility of Guinea pigs to experimental infection with HCC virus. Arch. ges. Virusforsch. 1958, 8, 600.
Schindler, R.: Serologische Untersuchungen über die Hepatitis contagiosa canis (HCC). Mh.prakt. Tierheilk. 1952, 4, 481.
Tajima, M., T. Motohashi and T. Samejima: Electron microscopy of infectious canine hepatitis virus grown in cultures of canine kidney cells. Amer. J. vet. Res. 1961, 22, 236.
Van Der Veen, J. and G. Van Der Ploeg: An outbreak of pharyngo-conjunctival fever caused by types 3 and 4 adenovirus at Waalwijk, The Netherlands, Amer. J. Hyg. 1958, 68, 95.
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Salenstedt, C.R. Studies on the Virus of Hepatitis Contagiosa Canis (HCC). Acta Vet Scand 4, 371–383 (1963). https://doi.org/10.1186/BF03547197
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DOI: https://doi.org/10.1186/BF03547197