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The Toxic Effects of Dimethylnitrosamine In Sheep

Toksisk virkning au dimethylnitrosamin på sau

Acta Veterinaria Scandinavica volume 15, pages 533–543 (1974)Cite this article

Abstract

Experimental feeding, dealing with long term ingestion of small daily doses of DMNA has clearly demonstrated the cumulative toxic effect of this compound in sheep. A threshold level, somewhere between 0.10 mg and 0.15 mg DMNA/kg body weight per day, can be defined below which no toxicity even in quite long-lasting feeding can be demonstrated. Above this level toxicity changes will develop, clinically in most cases, anatomically in all, if the exposure time is long enough. If the daily intake of DMNA is over this threshold level, 21–40 mg DMNA/kg body weight, it usually causes liver disease and death in sheep. Neoplastic lesions were not produced, the experimental period, however, was below the latency period for carcinogenesis.

Sammendrag

Foringsforsøk har vist at daglig opptak av dimethylnitrosamin (DMNA) har en kumulativ toksisk effekt på sau hvis dagsdosene er over en terskelverdi på ca. 0,1–0,15 mg DMNA/kg levende vekt, mens dagsdoser mindre enn denne terskelverdi ikke ga kliniske symptomer eller pato-anatomiske forandringer. Dagsdoser over terskelverdien forårsaket toksiske forandringer, spesielt i leveren, leverinsuffisiens og d0d når dyret har opptatt 21–40 mg DMNA/kg levende vekt. Svulster forekom ikke på forsøkssauene, men forsøkstiden var langt under den latensperiode som en må forvente for svulstdanneise.

References

  • Ayanaba, A., W. Verstraete & M. Alexander: Possible microbial contribution to nitrosamine formation in sewage and soil. J. nat. Cancer Inst. 1973, 50, 811–813.

    Article  CAS  Google Scholar 

  • Crosby, N. T., J. K. Foreman, J. F. Palframan & R. Sawyer: Estimation of steam-volatile N-nitrosamines in foods at the 1 microgram/kg level. Nature (Lond.) 1972, 238, 342–343.

    Article  CAS  Google Scholar 

  • DuPlessis, L. S., J. R. Nunn & W. A. Roach: Carcinogen in a Traskeian Bantu food additive. Nature (Lond.) 1969, 222, 1198–1199.

    Article  CAS  Google Scholar 

  • Ender, F. & L. Ceh: Occurrence and determination of nitrosamines in foodstuffs for human and animal nutrition. Alkylierend Wirkende Verbindungen, Second Conf. on Tobacco Research, Freiburg, Germany 1967, p. 83–91.

  • Ender, F. & L. Ceh: Conditions and chemical reaction mechanisms by which nitrosamines may be formed in biological products with reference to their possible occurrence in food products. Z. Lebensmitt.-Untersuch. 1971, 145, 133–142.

    Article  CAS  Google Scholar 

  • Fazio, T., J. N. Damico, J. W. Howard, R. H. White & J. O. Watts: Gas chromatographic determination and mass spectrometric confirmation of N-nitrosodimethylamine in smoke-processed marine fish. J. agriv. Food Chem. 1971, 19, 250–255.

    Article  CAS  Google Scholar 

  • Fiddler, W., R. C. Doerr, J. R. Ertel & A. E. Wasserman: Gas-liquid chromatographic determination of N-nitrosodimethylamine in ham. J. Ass. off. analyt. Ghem. 1971, 54, 1160–1163.

    CAS  Google Scholar 

  • Fong, Y. Y. & E. O’F. Walsh: Carcinogenic nitrosamines in cantonese salt-dried fish. Lancet 1971, ii, 1032.

  • Harington, J. L., J. R. Nunn & L. Irwig: Dimethylnitrosamine in the human vaginal vault. Nature (Lond.) 1973, 241, 49–50.

    Article  CAS  Google Scholar 

  • Klubes, P., I. Cerna, A. D. Rabinowitz & W. R. Jondorf: Factors affecting dimethylnitrosamine formation from simple precursors by rat intestinal bacteria. Food Gosmet. Toxicol. 1972, 10, 757–767.

    Article  CAS  Google Scholar 

  • Koppang, N.: Proc. 9th Nord. Vet. Gongr., Copenhagen 1962, pp. 777–778.

  • Koppang, N.: An outbreak of toxic liver injury in ruminants. Nord. Vet.-Med. 1964, 16, 305–322.

    Google Scholar 

  • Koppang, N.: Forgiftning hos husdyr forârsaket av sildemel. (Toxic hepatosis in animals caused by herring meal). Proc. 10th Nord. Vet. Gongr., Stockholm 1966, pp. 554–561.

  • Koppang, N.: Toxic hepatosis in ruminants and fur animals caused by dimethylnitrosamine. Acta path. microbiol. scand., Suppl., 1967, 187, 58–59.

    Google Scholar 

  • Koppang, N.: The hepatotoxic and carcinogenic actions of dimethylnitrosamine. Acta path. microbiol. scand. Section A and B, Suppl., 1970, 215, 30.

    Google Scholar 

  • Lydersen, D. L. & K. Nagy: Polarographische Bestimmung von Dimethylnitrosamin in Fischprodukten. (Polarographic testing of dimethylnitrosamine in fish products). Z. analyt. Chem. 1967, 230, 277–282.

    CAS  Google Scholar 

  • Magee, P. N. & J. M. Barnes: The production of malignant primary hepatic tumours in the rat by feeding dimethylnitrosamine. Brit. J. Cancer 1956, 10, 114–122.

    Article  CAS  Google Scholar 

  • McGlashan, N. D., C. L. Walters & A. E. M. McLean: Nitrosamines in African alcoholic spirits and oesophageal cancer. Lancet 1968, ii, 1017.

  • Möhler, K. & O. L. Mayrhofer: Nachweis und Bestimmung von Nitrosaminen in Lebensmitteln. (Detection and testing of nitrosamine in foods). Z. Lebensmitt.-Untersuch. 1968, 135, 313–318.

    Article  Google Scholar 

  • Sander, J.: Kann Nitrit in der menschlichen Nahrung Ursache einer Krebsentstehung durch Nitrosaminbildung sein? (Nitrite in the food as the cause of cancer by nitrosamine formation). Arch. Hyg. (Berlin) 1967, 151, 22–28.

    CAS  Google Scholar 

  • Sander, J.: Untersuchungen über die Entstehung cancerogener Nitrosoverbindungen im Magen von Versuchstieren und ihre Bedeutung für den Mensenen. 1. Mitteilung. (Studies on the origin of cancerogenic nitrosamines in the stomach of laboratory animals as a possible human model). Arzneimittel-Forsch. 1971, 21, 1572.

    CAS  Google Scholar 

  • Scolder, J. & G. Bürkle: Induktion maligner Tumoren bei Ratten durch gleichzeitige Verfütterung von Nitrit und sekundären Aminen. (Induction of malignant tumours in rats by simultaneous feeding of nitrites and secondary amines). Z. Krebsforsch. 1969, 73, 54–66.

    Google Scholar 

  • Sander, J., F. Schweinsberg & H. P. Menz: Untersuchungen über die Entstehung cancerogener Nitrosamine im Magen. (Studies on the origin of cancerogenic nitrosamines in the stomach). Hoppe-Seylers Z. physiol. Chem. 1968, 349, 1691–1697.

    CAS  Google Scholar 

  • Sen, N. P.: The evidence for the presence of dimethylnitrosamine in meat products. Food Cosmet. Toxicol. 1972, 10, 219–223.

    Article  CAS  Google Scholar 

  • Tollersrud, S.: Studies on some serum enzyme assays and their diagnostic value in nutritional disease of ruminants and pigs. Thesis. Universitetsforlaget, Oslo 1970.

  • Wasserman, A. E., W. Fiddler, R. C. Doerr, S. F. Osman & C. J. Dooley: Dimethylnitrosamine in frankfurters. Food Cosmet. Toxicol. 1972, 10, 681–684.

    Article  CAS  Google Scholar 

  • Wolff, I. A. & A. E. Wasserman: Nitrates, nitrites and nitrosamines. Science 1972, 177, 15–19.

    Article  CAS  Google Scholar 

Download references

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  1. Department of Pathology, Veterinary College of Norway, Oslo Dep., Oslo 1, Norway

    Nils Koppang

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Koppang, N. The Toxic Effects of Dimethylnitrosamine In Sheep. Acta Vet Scand 15, 533–543 (1974). https://doi.org/10.1186/BF03547224

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Acta Veterinaria Scandinavica

ISSN: 1751-0147