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Some Reproductive and Clinical Aspects of Endotoxins in Cows with Special Emphasis on The Role of Prostaglandins

Effekter av endotoxiner på prostaglandin frisättning, lutealfunktion och klinik hos kor.

Acta Veterinaria Scandinavica volume 25, pages 365–377 (1984)Cite this article

Abstract

Endotoxin from Salmonella typhimurium was administered intravenously to 3 cows. It produced an immediate increase in blood prostaglandin metabolite levels causing either luteolysis or a temporary drop in progesterone concentrations depending on the degree of prostaglandin synthesis. The clinical effects on heart rate, respiratory rate, ruminai contraction rate, body temperature and other general signs were studied. The cows showed a transient increase in heart and respiratory rate and a decrease in ruminai activity. The temperature responded by a small initial decrease and a secondary increase. Other symptoms included muscular weakness and shivering, excessive salivation and diarrhoea. Plasma Ca levels showed a direct decrease and then slowly increased to initial levels in the course of several days. The role of endotoxins in Gram-negative infections and their mode of action on the body are discussed with special emphasis on the role of prostaglandins on reproductive function.

Sammanfattning

Endotoxin från Salmonella typhimurium gavs intravenöst till 3 cyklande kor. Prostaglandinfrisättningen bestämdes genom att mäta 15-keto-13,14-dihydro-PGF2α, och gulkroppsfunktionen följdes med progesteronbestämning i plasma. Beroende på mängden frisatt prostaglandin iakttogs antingen luteolys med förkortning av östralcykeln eller en tillfällig sänkning i progesteronnivåerna. Kliniska effekter på hjärt-, andnings- och våmfrekvens samt temperatur registrerades. En övergående ökning av hjärt- och andningsfrekvens samt en sänkning av våmfrekvensen iakttogs. Temperaturen sjönk först för att sedan öka. Bland övriga symtom sågs muskelsvaghet och skakningar, ökad salivering och diarré. Plasma Ca-koncentrationen sjönk omedelbart för att sedan återgå till ursprungsnivåerna under loppet av fiera dagar. Endotoxinernas verkningsmekanism i kroppen vid Gram-negativa infektioner diskuteras speciellt med avseende på prostaglandinernas roll.

References

  • Athens, J. W., O. P. Haab, S. O. Raab, A. M. Mauer, H. Ashenbrucker, G. E. Cartwright & M. M. Wintrobe: Leukokinetic studies. IV. The total blood circulating and marginal granulocyte pools and the granulocyte turnover rate in normal subjects. J. clin. Invest. 1961, 40, 989–995.

    CAS  PubMed  Google Scholar 

  • Bosu, W. T. K., L.-E. Edqvist, P. Lindberg, K. Martinsson & E. D. B. Johansson: The effect of various dosages of lynesterol on the plasma level of oestrogens and progesterone during the menstrual cycle in the rhesus monkey. Contraception 1976, 13, 677–684.

    Article  CAS  Google Scholar 

  • Carroll, E. J., O. W. Schalm & J. Lasmanis: Experimental coliform (Aerobacter aerogens) mastitis: Characteristics of the endotoxin and its role in pathogenesis. Amer. J. vet. Res. 1964, 25, 720–726.

    CAS  PubMed  Google Scholar 

  • Culbertson, R. Jr. & B. I. Osburn: The biological effects of bacterial endotoxin: A short review. Vet. Sci. Commun. 1980, 4, 3–14.

    CAS  Google Scholar 

  • Eberhart, R. J., R. P. Natzke, F. H. S. Newbould, B. Nonnecke & R. P. Thompson: Coliform mastitis — A review. J. Dairy Sci. 1979, 62, 1–22.

    Article  Google Scholar 

  • Elmore, R. G., C. E. Martin & J. N. Berg: Absorption of Escherichia coli endotoxin from the mammary glands and uteri of early postpartum sows and gilts. Theriogenology 1978, 10, 439–447.

    Article  Google Scholar 

  • Estergreen, J. L. Jr., O. L. Frost, W. R. Gomes, R. E. Erb & J. F. Bullard: Effect of ovariectomy on pregnancy maintenance and parturition in dairy cows. J. Dairy Sci. 1967, 50, 1293–1295.

    Article  Google Scholar 

  • Fletcher, J. R. & P. W. Ramwell: Modification, by aspirin and indomethacin, of the haemodynamic and prostaglandin releasing effects of E. coli endotoxin in the dog. Brit. J. Pharmac. 1977, 61, 175–181.

    CAS  Google Scholar 

  • Fredriksson, G., H. Kindahl & L.-E. Edqvist: Endotoxin induced prostaglandin release and corpus luteum function in goats. Anim. Reprod. Sci. 1985, 8, in press.

  • Frost, A. J., A. W. Hill & B. E. Brooker: The early pathogenesis of bovine mastitis due to Escherichia coli. Proc. Royal Soc. London B. 1980, 209, 431–439.

    CAS  Google Scholar 

  • Frost, A. J., A. W. Hill & B. E. Brooker: Pathogenesis of experimental bovine mastitis following a small inoculum of Escherichia coli. Res. Vet. Sci. 1982, 33, 105–112.

    Article  CAS  Google Scholar 

  • Griel, L. C. Jr., A. Zarkower & R. J. Eberhart: Clinical and clinicopathological effects of Escherichia coli endotoxin in mature cattle. Canad. J. comp. Med. 1975, 39, 1–6.

    Google Scholar 

  • Harper, M. J. K., R. R. Bodhke & W. E. Friedrichs: Effect of endotoxin treatment on prostaglandin metabolism by rabbit uterus and oviduct. J. Reprod. Fert. 1980, 58, 101–108.

    Article  CAS  Google Scholar 

  • Hill, A. W., A. L. Shears & K. G. Hibbitt: The pathogenesis of experimental Escherichia coli mastitis in newly calved dairy cows. Res. Vet. Sci. 1979, 26, 97–101.

    Article  CAS  Google Scholar 

  • Horton, E. W. & N. L. Poyser: Uterine luteolytic hormone: A physiological role for prostaglandin F2α. Physiol. Rev. 1976, 56, 595–651.

    Article  CAS  Google Scholar 

  • Itaya, K. & M. Ui: A new micromethod for the colorimetric determination of inorganic phosphate. Clin. Chim. Acta 1966, 14, 361–366.

    Article  CAS  Google Scholar 

  • Kindahl, H., L.-E. Edqvist, E. Granström & A. Bane: The release of prostaglandin F2 α, as reflected by 15-keto-13,14-dihydroprostaglandin F2 α in the peripheral circulation during normal luteolysis in heifers. Prostaglandins 1976, 11, 871–878.

    Article  CAS  Google Scholar 

  • Lindberg, A. A., G. E. Sheldon éc S. B. Svenson: Induction of endotoxin tolerance with nonpyrogenic O-antigenic oligosaccharide-protein conjugates. Infect. Immun. 1983, 41, 883–895.

    Google Scholar 

  • Luthman, J., S. O. Jacobsson & H. Kindahl: The influence of prostaglandins on calcium homeostasis in dairy cows. Zbl. Vet. Med. 1983, 30, 1–9.

    Google Scholar 

  • van Miert, A. S. J. P. A. M., C. T. M. van Duin, J. H. M. Verheijden & A. J. H.: Schtoman: Endotoxin-induced fever and associated haematological and blood biochemical changes in the goat: the effect of repeated administration and the influence of flurbiprofen. Res. Vet. Sci. 1982, 33, 248–255.

    Article  CAS  Google Scholar 

  • Moore, J. N., H. E. Garner, J. E. Shapland & D. G. Hatfield: Prevention of endotoxin-induced arterial hypoxaemia and lactic acidosis with flunixin meglumine in the conscious pony. Equine Vet. J. 1981, 13, 95–98.

    Article  CAS  Google Scholar 

  • Nachreiner, R. F., M. C. Garcia & O. J. Ginther: Clinical, hematologic, and blood chemical changes in swine given endotoxin (Escherichia coli) during the immediate postpartum period. Amer. J. vet. Res. 1972, 33, 2489–2499.

    CAS  PubMed  Google Scholar 

  • Nakano, J.: Cardiovascular actions. In: Ramwell, P. W. (ed.) : The Prostaglandins, vol. 1. Plenum Press, New Work-London 1973, pp. 238–316.

  • Bobert, A.: Antisecretory, antiulcer, cytoprotective and diarrheogenic properties of prostaglandins. Advances in Prostaglandin and Thromboxane Research 1976, 2, 507–520.

    Google Scholar 

  • Simesen, M. G.: Calcium, phosphorus, and magnesium metabolism. In: Kaneko, J. (ed) : Clinical Riochemistry of Domestic Animals, 3rd edn., Academic Press, New York 1980, pp. 575–648.

  • Skarnes, B.: Host defense against bacterial endotoxemia: Mechanism in normal animals. J. exp. Med. 1970, 32, 300–315.

    Article  Google Scholar 

  • Skarnes, B., S. K. Brown, S. S. Hull & J. A. McCracken: Role of prostaglandin E in the biphasic fever response to endotoxin. J. exp. Med. 1981, 154, 1212–1224.

    Article  CAS  Google Scholar 

  • Smith, A. P.: Lungs. In: Ramwell, P. W. (ed) : The Prostaglandins. Plenum Press, New York-London 1973, pp. 203–218.

  • Svenson, S. B. & A. A. Lindberg: Immunochemistry of Salmonella Oantigens: preparations of an octasaccharide-bovine serum albumin immunogen representative of Salmonella serogroup B Oantigen and characterization of the antibody response. J. Immunol. 1978, 120, 1750–1757.

    CAS  PubMed  Google Scholar 

  • Trudeau, D. L. & E. F. Freier: Determination of calcium in urine and serum by atomic absorption spectrophotometry (AAS). Clin. Chem. 1967, 13, 101–114.

    Article  CAS  Google Scholar 

  • Veenendaal, G. H., F. M. A. Wout ersen-van Nijnanten, C. T. M. van Duin & A. S. J. P. A. M. van Miert: Role of circulating prostaglandins in the genesis of pyrogen (endotoxin)-induced ruminai stasis in conscious goats. J. vet. Pharmacol. Therap. 1980, 3, 59–68.

    Article  CAS  Google Scholar 

  • Verheijden, J. H. M., A. S. J. P. A. M. van Miert, A. J. H. Shotman & C. T. M. van Duin: Plasma zinc and iron concentrations as measurements for evaluating the influence of endotoxin-neutralinzing agents in Escherichia coli endotoxin-induced mastitis. Amer. J. vet. Res. 1982, 43, 724–728.

    CAS  PubMed  Google Scholar 

  • Zarkower, A., R. J. Eberhart & L. Griel: Hypocalcemia and other effects of E. coli endotoxin in cattle. Proc. VII International meeting on disease of cattle. World Association for Buiatrics Congress, London 1972, pp. 339–347.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Gunnar Predriksson

  2. Department of Clinical Chemistry, College of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Gunnar Predriksson

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  1. Gunnar Predriksson
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This study was supported by grants from the Swedish Council for Forestry and Agricultural Research.

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Predriksson, G. Some Reproductive and Clinical Aspects of Endotoxins in Cows with Special Emphasis on The Role of Prostaglandins. Acta Vet Scand 25, 365–377 (1984). https://doi.org/10.1186/BF03547251

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Acta Veterinaria Scandinavica

ISSN: 1751-0147