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An Enzyme-Linked Immunosorbent Assay (Elisa) for The Primary Diagnosis of Foot-And-Mouth Disease Characterization and Comparison with Complement Fixation

En ELISA til diagnosticering af mund-og-klovesyge. Karakterisering og sammenligning med komplementbinding.

Abstract

A sandwich type ELISA for foot-and-mouth disease (FMD) virus types O, A and C was established, using a combination of rabbit anti-146 S and guinea pig hyperimmune antibodies. This method was found to be highly efficient for the detection of both 146 S particles and 12 S subunits. The ELISA was approximately 500 times more sensitive than complement fixation (CF) when examining epithelial samples of FMD vesicles. An early primary diagnosis of FMD was obtained by both CF and ELISA in 19 out of 21 confirmed cases. The remaining 2 cases were initially negative in CF but positive in ELISA.

Sammendrag

Der beskrives en sandwich ELISA til påvisning af mund- og klovesyge virus type O, A og C ved hjælp af hyperimmune marsvinesera og kaninsera fremstillet ved immunisering med rensede 146 S viruspartikler. Metoden fandtes velegnet til påvisning af både intakte 146 S viruspartikler og degraderede viruspartikler (12 S partikler).

ELISA teknikken var ca. 500 gange så følsom som komplementbindingsprøven til påvisning af mund- og klovesygevirus (antigen) i aftemateriale.

I 19 ud af 21 verificerede tilfælde var dot muligt at stille en tidlig mund- og klovesyge diagnose ved både komplementbinding og ELISA. I de to sidste tilfaelde blev en tidlig diagnose alene stillet ved ELISA.

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Correspondence to P. Have.

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Have, P., Lei, J.C. & Schjerning-Thiesen, K. An Enzyme-Linked Immunosorbent Assay (Elisa) for The Primary Diagnosis of Foot-And-Mouth Disease Characterization and Comparison with Complement Fixation. Acta Vet Scand 25, 280–296 (1984). https://doi.org/10.1186/BF03547271

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Keywords

  • FMD
  • virus
  • type O1
  • type A10
  • type C
  • double sandwich technique
  • 146 S particles
  • 12 S subunits
  • field samples
  • sensitivity
  • specificity
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