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An Enzyme-Linked Immunosorbent Assay (Elisa) for The Primary Diagnosis of Foot-And-Mouth Disease Characterization and Comparison with Complement Fixation

En ELISA til diagnosticering af mund-og-klovesyge. Karakterisering og sammenligning med komplementbinding.

Acta Veterinaria Scandinavica volume 25, pages 280–296 (1984)Cite this article

Abstract

A sandwich type ELISA for foot-and-mouth disease (FMD) virus types O, A and C was established, using a combination of rabbit anti-146 S and guinea pig hyperimmune antibodies. This method was found to be highly efficient for the detection of both 146 S particles and 12 S subunits. The ELISA was approximately 500 times more sensitive than complement fixation (CF) when examining epithelial samples of FMD vesicles. An early primary diagnosis of FMD was obtained by both CF and ELISA in 19 out of 21 confirmed cases. The remaining 2 cases were initially negative in CF but positive in ELISA.

Sammendrag

Der beskrives en sandwich ELISA til påvisning af mund- og klovesyge virus type O, A og C ved hjælp af hyperimmune marsvinesera og kaninsera fremstillet ved immunisering med rensede 146 S viruspartikler. Metoden fandtes velegnet til påvisning af både intakte 146 S viruspartikler og degraderede viruspartikler (12 S partikler).

ELISA teknikken var ca. 500 gange så følsom som komplementbindingsprøven til påvisning af mund- og klovesygevirus (antigen) i aftemateriale.

I 19 ud af 21 verificerede tilfælde var dot muligt at stille en tidlig mund- og klovesyge diagnose ved både komplementbinding og ELISA. I de to sidste tilfaelde blev en tidlig diagnose alene stillet ved ELISA.

References

  • Abu Elzein, E. M. E. & J. R. Crowther: The specific detection of foot-and-mouth disease virus whole particle antigen (140 S) by enzyme labelled immunosorbent assay. J. Hyg. 1979, 83, 127–134.

    Article  Google Scholar 

  • Cartwright, B., W. G. Chapman & F. Brown: Serological and immunological relationship between the 146 S and 12 S particles of foot-and-mouth disease virus, J. gen. Virol. 1980, 50, 369–375.

    Article  CAS  Google Scholar 

  • Crowther, J. R. & E. M. E. Abu Elzein: Application of the enzyme-linked immunosorbent assay to the detection and identification of foot-and-mouth viruses. J. Hyg. 1979a, 83, 513–519.

  • Crowther, J. R. & E. M. E. Abu Elzein: Detection and quantification of foot-and-mouth disease virus by enzyme-linked immunosorbent assay techniques. J. gen. Virol. 1979b, 42, 579–602.

  • Hardy, M. M. & D. Moore: Neutralization of foot-and-mouth disease virus. I. Sensitization of the 140 S virion by antibody also reactive with the 12 S protein subunit. J. gen. Virol. 1981, 55, 415–427.

    CAS  Google Scholar 

  • Lei, J. C.: Report of the session of the research group of the standing committee of the European commission for the control of FMD. Uccle, Belgium, FAO, Rome, 1978.

  • McCullough, K. C. & R. Butcher: Monoclonal antibodies against foot-and-mouth disease virus 146 S and 12 S particles. Arch. Virol. 1982, 74, 1–9.

    Article  CAS  Google Scholar 

  • Meloen, R. H. & F. Briaire: A study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12 S subunits with antisera against the structural proteins. J. gen. Virol. 1980, 51, 107–116.

    Article  CAS  Google Scholar 

  • Ouldridge, E. P. Barnett & M. M. Rweyemamu: The relative efficiency of two ELISA techniques for the titration of FMD antigen. In: THE ELISA: Enzyme-linked immunosorbent assay in veterinary research and diagnosis (ed. R. C. Wardly & J. R. Crowther). Current topics in veterinary medicine and animal science vol. 22. Martinus Nijhoff Publ. The Hague 1982.

  • Schjerning-Thiesen, K.: Differentiation of virus types in foot-and-mouth disease (FMD). Acta vet. Scand. 1964, 5, 133–145.

    Google Scholar 

  • Ternynck, T. & S. Avrameas: Polymerization and immobilization of proteins using ethylchloroformate and glutaraldehyde. Scan. J. Immunol, suppl. 1976, 3, 29–35.

    Article  CAS  Google Scholar 

  • Traub, E. & H. Möhlmann: Typebestimmung bei maul- und klauen-seuche mit hilfe der komplementbindungsprobe. (Typing of foot-and-mouth disease by complement fixation). Z. B1. Bakt. Abt. I. 1943, 150, 289–310.

    CAS  Google Scholar 

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Authors and Affiliations

  1. State veterinary Institute for Virus Research, Lindholm, Denmark

    P. Have, J. C. Lei & K. Schjerning-Thiesen

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  1. P. Have
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  2. J. C. Lei
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  3. K. Schjerning-Thiesen
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Have, P., Lei, J.C. & Schjerning-Thiesen, K. An Enzyme-Linked Immunosorbent Assay (Elisa) for The Primary Diagnosis of Foot-And-Mouth Disease Characterization and Comparison with Complement Fixation. Acta Vet Scand 25, 280–296 (1984). https://doi.org/10.1186/BF03547271

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Acta Veterinaria Scandinavica

ISSN: 1751-0147