Skip to main content
Download PDF
  • Published:

Enterotoxigenic Escherichia Coli in The Jejunum of Piglets with Neonatal Diarrhea

Frekvens og diagnostikk av enterotoksigene Escherichia coli i tynntarmen hos gris døde av diare.

Acta Veterinaria Scandinavica volume 25, pages 21–28 (1984)Cite this article

Abstract

Thirty-two Escherichia coli colonies were taken from the primary step of cultivation of the jejunal contents of each of 10 dead piglets which had suffered from diarrhea. The organisms of each colony were examined for the presence of adhesion fimbria (F4 (K88) and F5 (K99)), production of heat-stable and heat-labile enterotoxin and of colicins.

The presence of heat-labile enterotoxin in the intestinal content of the necropsied pigs was also tested, and results correlated with enterotoxin production of the isolated E. coli strains. In all but 3 pigs, 50–80 % of the E. coli strains were found to produce one or both of the enterotoxins and to possess the F4 of the F5 antigen. All bacteria producing both heat-labile and heat-stable enterotoxin proved to belong toi O group 149 and to possess the F4 antigen. Strains from 1 pig belonged to O group 64 and possessed the F5 antigen; these bacteria produced heat-stable enterotoxin only. Most of the enterotoxin-producing E. coli also produced colicins.

After each subcultivation, the strains produced less heat-labile enterotoxin, some becoming negative when assayed.

Sammendrag

Tretti-to kolonier av Escherichia coli ble isolert fra tynntarmen på hver av 10 døde grisunger og undersøkt med hensyn på spesifikke fimbrier, colicin og enterotoksin-produksjon. Tarminnholdet ble også undersøkt direkte for tilstedeværelse av termolabilt enterotoksin (LT). Fra 4 dyr fant en ingen enterotoksin produserende E. coli, hos 6 dyr fant en LT i tynntarminnholdet, og ca 80 % av E. coli koloniene var enterotoksiske for både LT og ST (termostabilt toksin). Disse bakteriene hadde også adhesjonsfimbrien F4 (K88) sammen med O antigen 149. En gris hadde E. coli med O antigen 64 og adhesjonsfimbrie F5 (K99). Disse dannet ikke LT, kun ST. De fleste enteropatogene E. coli var også colicin-produsenter. Etter subkultiveringer produserte stammene mindre LT, og noen som opprinnelig var positive ble etterhvert negative. Noen stammer forble imidlertid høyprodusenter av LT, selv etter mere enn 3 passasjer.

References

  • Bäck, E. R., R. Möllby, B. Kaijser, G. Stintzing, T. Wadström & D. Habte: Enterotoxigenic Escherichia coli and other gram-negative bacteria of infantile diarrhea: surface antigens, hemagglutinins, colonization factor antigen, and loss of enterotoxigenicity. J. infect. Dis. 1980, 142, 318–327.

    Article  Google Scholar 

  • Dean, A. G., Y. Ching, R. G. Williams & L. B. Harden: Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J. infect. Dis. 1971, 125, 407–411.

    Article  Google Scholar 

  • DeBoy, J. M., G. M. Thorne, C. F. Deneke & K. Wachmuth: Colonization factors and Escherichia coli belonging to enterotoxin-associated serotypes. Curr. Microbiol. 1981, 6, 49–53.

    Article  Google Scholar 

  • Edwards, P. R. & W. H. Ewing: Identification of Enterobacteriaceae 3rd ed. Burgess Publishing Company, Minn., USA 1972, p. 67–108.

  • Ellens, D. T., P. W. de Leeuw & H. Rozemond: Detection of the K99 antigen of Escherichia coli in calf faeces by enzyme-linked immunosorbent assay (ELISA). T. Diergeneesk. 1979, 104, suppl. 4, 169–175.

  • Evans, D. J., D. G. Evans, H. L. DuPont, F. Ørskov & I. Ørskov: Patterns of loss of enterotoxicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect. Immun. 1977, 17, 165–111.

    Article  Google Scholar 

  • Franklin, A., O. Söderlind & R. Möllby: Plasmids coding for enterotoxins, K88 antigen and colisins in porcine Escherichia coli strains of O-group 149. Med. Microbiol. Immunol. 1981, 170, 63–72.

    Article  CAS  Google Scholar 

  • Gaastra, W. & F. K. de Graaf: Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol. Rev. 1982, 42, 129–161.

    Article  Google Scholar 

  • Guinée, P. A. M., J. Veldkamp & W. H. Jansen: Improved Minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infect. Immun. 1977, 15, 676–678.

    Article  Google Scholar 

  • Lund, A., K. Fossum & E. Liven: Serological, enterotoxin producing and biochemical properties of Escherichia coli isolated from piglets with neonatal diarrhea in Norway. Acta vet. scand. 1982, 23, 79–87

  • McDonnell, M. M., H. L. Smith, G. A. Willshaw, A. M. Field & B. Rowe: Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: Comparison of their properties. Infect. Immun. 1981, 32, 927–936.

    Article  Google Scholar 

  • Morris, G. K., M. H. Merson, D. A. Sack, J. G. Wells, W. T. Martin, W. E. Dewitt, J. C. Feeley, R. B. Sack & D. M. Bessudo: Laboratory investigation of diarrhea in travelers to Mexico: Evaluation of methods for detecting enterotoxigenic Escherichia coli. J. clin. Microbiol. 1976, 3, 486–495.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Olsvik, Ø. & B. P. Berdal: Demonstration of Escherichia coli heat-labile enterotoxin using bacterial cell sonicates. Acta path, microbiol. immunol. Scand. Sect. B. 1982, 90, 319–321.

    CAS  Google Scholar 

  • Olsvik, Ø. & K. Fossum: Demonstration of Escherichia coli enterotoxin in the jejunum contents of pigs died of diarrhea. Proc. 14th Nordic Vet. Congr., Copenhagen 1982, p. 416.

  • Olsvik, Ø., O. T. Hushovd, B. P. Berdal, T. Bergan & M. Mathiesen: Production of enterotoxin by Escherichia coli at four, twenty-two and thirty-seven degrees centigrade. Eur. J clin. Microbiol. 1982, 1, 12–16.

    Article  CAS  Google Scholar 

  • Olsvik, Ø. & G. Kapperud: Enterotoxin production in milk at 22 and 4°C by Escherichia coli and Yersinia enterocolitica. Appl. Environ. Microbiol. 1982, 43, 997–1000.

  • Ørskov, F. & I. Ørskov: Serogrouping of E. coli. In: T. Bergan: Methods in Microbiology, vol 16. Academic Press, London 1983.

  • Reis, M. H. L., B. E. C. Guth, T. A. T. Gomes, J. Murahouschi & L. R. Trabulsi: Frequency of Escherichia coli strains producing heatlabile toxin or heatnstable toxin or both in children with and without diarrhea in San Paulo. J. clin. Microbiol. 1982, 15, 1062–1064.

    Article  CAS  Google Scholar 

  • Satterwith, T. K., D. G. Evans, H. L. DuPont & D. J. Evans jr.: Role of Escherichia coli colonization factor antigen in acute diarrhæa. Lancet 1978, vii, 181–184.

  • Smith, H. W. & M. B. Huggins: The influence of plasmid-dermined and other characteristics of enteropathogenic Escherichia coli on their ability to proliferate in the alimentary tracts of piglets, calves and lambs. J. med. Microbiol. 1977, 11, 471–492.

    Article  Google Scholar 

  • Söderlind, O.: Studies on Escherichia coli in pigs: II. Serological investigations. Zbl. Vet. Med. B. 1971, 18, 569–590.

    Article  Google Scholar 

  • Williams, P. H., M. I. Sedgwick, N. Evans, P. J. Turner, R. H. George & A. S. McNeish: Plasmid mediated mucosal adherence in human enteropathogenic Escherichia coli. Lancet 1977, i, 1151.

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, the Norwegian College of Veterinary Medicine, Oslo, Norway

    Ørjan Olsvik & Kåre Fossum

  2. Norwegian Defence Microbiological Laboratory, National Institute of Public Health, Oslo, Norway

    Ørjan Olsvik & Kåre Fossum

Authors
  1. Ørjan Olsvik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kåre Fossum
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Olsvik, Ø., Fossum, K. Enterotoxigenic Escherichia Coli in The Jejunum of Piglets with Neonatal Diarrhea. Acta Vet Scand 25, 21–28 (1984). https://doi.org/10.1186/BF03547275

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547275

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147