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Serological Characterization of 8 Haemophilus Pleuropneumoniae Strains and Proposal of a New Serotype: Serotype 8
Serologisk karakterisering af 8 Haemophilus pleuropneumoniae stammer og forelæggelse af en ny serotype, serotype 8.
Acta Veterinaria Scandinavica volume 25, pages 96–106 (1984)
Abstract
Eight strains of Haemophilus pleuropneumoniae isolated from 8 herd outbreaks of pleuropneumonia in pigs were studied by means of the slide agglutination test, the tube agglutination test, the IHA test and by gel diffusion.
The 8 strains were antigenically homogeneous and serologically distinct from serotypes 1 through 7. It is therefore proposed to refer these strains to a new serotype: serotype 8, with strain 405 as the type strain.
In addition to the serotype-specific capsular antigens, capsular antigen of serotype 3 (strain 1421) and serotype 6 (strain Femø) could be demonstrated in the 8 strains by means of the IHA test and by gel diffusion analyses.
Sammendrag
Otte Haemophilus pleuropneumoniae stammer, isoleret fra 8 besætningsudbrud af pleuropneumoni, blev undersøgt serologisk ved hjælp af objektglasagglutination, reagensglasagglutination, indirekte hemagglutination (IHA) og gel diffusion.
De 8 stammer udgjorde en homogen gruppe i antigen henseende og var serologisk forskellige fra de hidtil kendte serotyper 1 til 7. Gruppen er derfor blevet identificeret som en ny serotype, serotype 8 med stamme 405 som typestamme.
Ved hjælp af gel diffusion og IHA tests blev det påvist, at de 8 stammer, foruden serotype-specifikke kapselantigener, også indeholdt kapselantigener af serotype 3 (stamme 1421) og serotype 6 (stamme Femø).
References
Biberstein, E. L., A. Gunnarsson & B. Hurvell: Cultural and biochemical criteria for the identification of Haemophilus spp. from swine. Amer. J. vet. Res. 1977, 38, 7–11.
Branefors-Helander, P.: Serological studies of Haemophilus influenzae. I. Extracellular antigens of capsulated strains, types a to f, and two non-capsulated variants. Int. Arch. Allergy 1972, 43, 533–547.
Branefors-Helander, P.: Serological studies of Haemophilus influenzae. III. The endotoxic effect of various antigen preparations and the relation between this effect and demonstrable precipitinogens. Int. Arch. Allergy 1973, 44, 585–600.
Branefors-Helander, P., C. Erbing, L. Kenne & B. Lindberg: Structural studies of the capsular antigen from Haemophilus influenzae type b. Acta chem. scand. Sect. B. 1976, 30, 276–277.
Gunnarsson, A., E. L. Biberstein & B. Hurwell: Serologic studies on porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae). Agglutination reactions. Amer. J. vet. Res. 1977, 38, 1111–1114.
Keogh, E. V., E. A. North & M. F. Warburton: Adsorption of bacterial polysaccharides to erythrocytes. Nature 1948, 161, 687–688.
Kilian, M.: A taxonomic study of the genus Haemophilus with the proposal of a new species. Thesis, Århus 1976.
Nicolet, J.: Sur l’hemophilose du porc. III. Différenciation sérologique de Haemophilus parahaemolyticus (Haemophilus infections in pigs. III. Serological studies on Haemophilus parahaemolyticus). Zbl. Bakt. 1971, 216, 487–495.
Nielsen, R.: Serological and immunological studies of pleuropneumonia of swine caused by Haemophilus parahaemolyticus. Acta vet. scand. 1974, 15, 80–89.
Nielsen, R.: Haemophilus pleuropneumoniae infection in pigs. Thesis 1982. Commissioned by C. F. Mortensen A/S, Bülowsvej 5 C, DK-1870 Copenhagen V, Denmark.
Rosendal, S. & A. Boyd: Haemophilus pleuropneumoniae serotyping. J. clin. Microbiol. 1982, 16, 840–843.
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Nielsen, R., O’Connor, P.J. Serological Characterization of 8 Haemophilus Pleuropneumoniae Strains and Proposal of a New Serotype: Serotype 8. Acta Vet Scand 25, 96–106 (1984). https://doi.org/10.1186/BF03547283
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DOI: https://doi.org/10.1186/BF03547283