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Serological Characterization of 8 Haemophilus Pleuropneumoniae Strains and Proposal of a New Serotype: Serotype 8

Serologisk karakterisering af 8 Haemophilus pleuropneumoniae stammer og forelæggelse af en ny serotype, serotype 8.

Abstract

Eight strains of Haemophilus pleuropneumoniae isolated from 8 herd outbreaks of pleuropneumonia in pigs were studied by means of the slide agglutination test, the tube agglutination test, the IHA test and by gel diffusion.

The 8 strains were antigenically homogeneous and serologically distinct from serotypes 1 through 7. It is therefore proposed to refer these strains to a new serotype: serotype 8, with strain 405 as the type strain.

In addition to the serotype-specific capsular antigens, capsular antigen of serotype 3 (strain 1421) and serotype 6 (strain Femø) could be demonstrated in the 8 strains by means of the IHA test and by gel diffusion analyses.

Sammendrag

Otte Haemophilus pleuropneumoniae stammer, isoleret fra 8 besætningsudbrud af pleuropneumoni, blev undersøgt serologisk ved hjælp af objektglasagglutination, reagensglasagglutination, indirekte hemagglutination (IHA) og gel diffusion.

De 8 stammer udgjorde en homogen gruppe i antigen henseende og var serologisk forskellige fra de hidtil kendte serotyper 1 til 7. Gruppen er derfor blevet identificeret som en ny serotype, serotype 8 med stamme 405 som typestamme.

Ved hjælp af gel diffusion og IHA tests blev det påvist, at de 8 stammer, foruden serotype-specifikke kapselantigener, også indeholdt kapselantigener af serotype 3 (stamme 1421) og serotype 6 (stamme Femø).

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Nielsen, R., O’Connor, P.J. Serological Characterization of 8 Haemophilus Pleuropneumoniae Strains and Proposal of a New Serotype: Serotype 8. Acta Vet Scand 25, 96–106 (1984). https://doi.org/10.1186/BF03547283

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