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A Study of the Development of Endotoxin-induced Inflammation in the Bovine Teat

En studie av utvecklingen av endotoxininducerad inflammation i den bovina spenen

Acta Veterinaria Scandinavica volume 33, pages 283–295 (1992)Cite this article

Abstract

Endotoxin-induced local inflammation was studied by frequent samplings in a bovine teat cistern model, which provides a unique possibility for in vivo studies of reactions in the teat without interference from the mammary gland.

A rapid inflammatory response of rather short duration was elicited after endotoxin administration. An initial increase in the concentrations of bovine serum albumin and N-acetyl-β-D-glucosaminidase, indicating a disturbance in the epithelial integrity, was observed between 1 and 1.5 h post infusion (p.i.). Approximately 0.5 h later, the first influx of leukocytes, mainly neutrophils, appeared. The neutrophils tended to enter the teat cistern in several peaks occurring between 2.5 and 5 h p.i.. The sampling procedure decreased the accumulation of cells by approximately 40%, which was probably due to the removal of inflammatory mediators at an early stage. The parallel use of 2 teats instead of 1 had no major influence on the inflammatory process. This teat cistern model and the experimental procedure used should be suitable for further studies of the development of local inflammation.

Sammanfattning

Bovin endotoxininducerad lokal inflammation i spenen studerades med hjalp av en spencisternmodell. Denna modell ger en unik möjlighet till studier av spenreaktioner in vivo utan påverkan från juverkörteln.

Ett snabbt inflammatoriskt svar med relativt kort duration utlöstes. Ett ökat innehåll av bovint serum albumin och N-acetyl-β-D-glucosaminidase i spencisternen, vilket indikerade en störning i epitelets integritet, noterades redan 1 till 1.5 t efter infusion. Cirka 0.5 t senare sags det första inflödet av leukocyter, ffa neutrofiler. Cellinflödet tenderade att ske i flera omgångar melian 2.5 och 51 efter infusion. Provtagningsförfarandet minskade cellansamlingen i spenen med cirka 40%, troligen pga avlägsnande av inflammationsmediatorer under initialskedet. Samtidigt användande av 2 spenar istället för 1 hade ingen större inverkan på inflammationsprocessen.

Denna spencisternmodell bör vara lämplig för vidare studier av lokal bovin inflammation.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Department of Veterinary Microbiology, Section of Clinical Microbiology, and Department of Veterinary Medical Chemistry, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7039, S-750 07, Uppsala, Sweden

    K. Persson & C. Hallén Sandgren

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  1. K. Persson
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  2. C. Hallén Sandgren
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Persson, K., Hallén Sandgren, C. A Study of the Development of Endotoxin-induced Inflammation in the Bovine Teat. Acta Vet Scand 33, 283–295 (1992). https://doi.org/10.1186/BF03547294

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Acta Veterinaria Scandinavica

ISSN: 1751-0147