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Impact of Incubator Type on the Yield of in Vitro Produced Bovine Blastocysts

Inkubatorens betydning for udbyttet af in vitro producerede kvæg blastocyster

Acta Veterinaria Scandinavica volume 33, pages 341–348 (1992)Cite this article

Abstract

Because of suboptimal in vitro production of bovine blastocysts a new incubator model (Mini) was tested against the traditional (Heraeus). The difference between their properties seemed only to be the volume of the incubator space. No difference was noted between the CO2 or the temperature, but the data clearly showed a highly significant increase of the blastocyst rates, 6% versus 51% in the Heraeus and the Mini incubator, respectively, calculated as blastocysts per cleaved embryos. It was concluded that the incubator type or model may be a very important part of the in vitro production of bovine embryos, although we were not able to pin point specific causes for this difference.

Sammenfatning

Kvæg blastocyster fremstillet ved reagensglasbefrugtning blev sammenlignet efter dyrkning i to forskellige kuldioxid inkubatorer. Bortset fra rumfanget var de to inkubatorer i princippet ens (CO2, temperatur, luftfugtighed), men udbyttet af blastocyster, beregnet som blastocyster pr totalt antal dyrkede æg, var 3% versus 39% i henholdsvis den store og den lille inkubator (p <0.0001). Indtil 8–16 celle stadiet udvikledes æggene stort set ens. I den store inkubator gik æggenes udvikling i stå ved 8–16 celle blok stadiet, mens æggene i den lille inkubator fortsatte til blastocyst stadiet. Årsagen til denne forskel kendes ikke, men kan måske skyldes en toksisk effekt af kobber, som beklæder den store Inkubators indre overflader.

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Authors and Affiliations

  1. Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Biilowsvej 13, DK-1870, Frederiksberg, Denmark

    B. Avery & T. Greve

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  1. B. Avery
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  2. T. Greve
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Avery, B., Greve, T. Impact of Incubator Type on the Yield of in Vitro Produced Bovine Blastocysts. Acta Vet Scand 33, 341–348 (1992). https://doi.org/10.1186/BF03547300

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Acta Veterinaria Scandinavica

ISSN: 1751-0147