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Bovine Uterine, Cervical and Ovarian Androgen Receptor Concentrations

Koncentrationen av androgen receptorer i livmodern, livmoderhalsen och ovarierna hos kor

Correlation with Estrogen and Progesterone Receptor Concentrations

Acta Veterinaria Scandinavica volume 33, pages 379–386 (1992)Cite this article

Abstract

Bovine cytosol androgen receptor (ARC) concentrations were examined simultaneously in various regions of the uterus and in ovarian tissues of cows, and were related to cytosol estrogen (ERC) and progesterone receptor (PRC) concentrations and circulating steroid levels. ERC concentrations were 3-7-fold and PRC concentrations 13-29-fold those of ARC in bovine endometrial and myometrial tissues. When serum progesterone levels were low, both endometrial and myometrial ARC, endometrial ERC, and endometrial and myometrial PRC concentrations were higher (p<0.05) than those observed during higher progesterone concentrations. Because serum 5α-dihydrotestosterone (5α-DHT) concentrations were higher during the luteal phase, it is possible that ARC was down-regulated by this natural ligand at this phase of the cycle. There were no differences between uterine horns in endometrial or myometrial ARC concentrations. Bovine cervical and ovarian stromal tissue also contained ARC, and the concentrations were about the same as in the endometrium and the myometrium. The relative binding affinities (RBAs) of some steroid hormones towards ARC in vitro were: the synthetic compound R1881 (146%), 5α-dihydrotestosterone (100%), testosterone (75%) while estradiol-17β, progesterone and dexamethasone had lower RBAs (2, <1, <1% respectively). Cytosol androgen receptor concentrations correlated significantly with cytosol progesterone (PRC) and estrogen receptor (ERC) concentrations, both in the endometrium and myometrium. These data show that androgens, such as 5α-DHT, may participate the endocrine regulation of bovine reproductive tissues.

Sammanfattning

Koncentrationerna av cytoplasmiska androgen receptorer (ARC) i kornas livmoder och ovarier studerades samtidigt med estrogen (ERC) och progesteronreceptorer (PRC) och jämfördes med steroid hormoner i blodserum. Koncentrationerna av ERC var 3–7 och PRC 13–29 gånger högre än motsvarande ARC koncentrationer både i endometrium och myometrium. Koncentrationerna av cytoplasmiska ARC i de undersökta kornas livmödrer varierade enligt brunstcykeln. Då progesteronhalten i blodserum var låg, var även 5α-dihydrotestosteronhalten (5α-DHT) låg - livmoderns ARC-koncentrationer i endometrium och myometrium var däremot på en högre nivå. Också ERC koncentrationerna i endometrium och PRC både i endometrium och myometrium var högre under denna period. Vid höjda serum progesteron och 5α-DHT koncentrationer minskade emellertid livmoderns ARC-halt samtidigt med ERC och PRC koncentrationerna. Antagligen berodde minskningen av ARC på att höjda 5α-DHT-koncentrationer verkade hämmande på ARC-nivån. ARC koncentrationerna i olika delar av livmodern befanns vara på samma nivå. En positiv korrelation mellan mängderna av androgen-, progesteron- och estrogenreceptorer i livmodern kunde påvisas. Syntetisk metyltrienolon (R1881) hade en högre och testosteron en mindre affinitet till ARC an 5α-DHT in vitro. Estradiol-17β, progesteron och dexametason hade endast en minimal affinitet till ARC.

References

  • Apter D, Jänne O, Karvonen P, Vihko R: Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000. Clin. Chem. 1976, 22, 32–38.

    Article  CAS  Google Scholar 

  • de Boer W, Lindh M, Bolt J, Brinkmann A, Mulder E: Characterization of the calf uterine androgen receptor and its activation to the deoxyribonucleic acid-binding state. Endocrinology 1986, 118, 851–861.

    Article  Google Scholar 

  • Bonne C, Raynaud J-P: Assay of androgen binding sites by exchange with methyltrienolone (R1881). Steroids 1976, 27, 497–507.

    Article  CAS  Google Scholar 

  • Bradford MM: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254.

    Article  CAS  Google Scholar 

  • Büchi KA, Weber E: Variation of cytoplasmic rat uterine androgen receptors. Moll. Cell. Endocrinol. 1983, 29, 295–307.

    Article  Google Scholar 

  • Chen TJ, Leavitt WW: Nuclear progesterone receptor in hamster uterus. Measurement by [3H]progesterone exchange during the estrous cycle. Endocrinology 1979, 104, 1588–1597.

    Article  CAS  Google Scholar 

  • Chang CH, Tindall DJ: Physicochemical characterization of the androgen receptor in rat uterine cytosol. Endocrinology 1983, 113, 1486–1493.

    Article  CAS  Google Scholar 

  • Clark J, Schrader W, O’Malley B: Mechanisms of steroid hormone action. In: Wilson J, Fortec D (eds): Textbook of Endocrinology. WB Saunders Company, Philadelphia 1985, pp. 33–75.

  • Ekman P, Barrack E, Walsh P: Simultaneous measurement of progesterone and androgen receptors in human prostate: A microassay. J. clin. Endocr Metab. 1982, 55, 1089–1099.

    Article  CAS  Google Scholar 

  • Gaubert CM, Tremblay RR, Dube JY: Effect of sodium molybdate on cytosolic androgen receptors in rat prostate. J. Steroid Biochem. 1980, 13, 931–937.

    Article  CAS  Google Scholar 

  • Hammond G, Ruokonen A, Kontturi M, Koskela E, Vihko R: The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. J. clin. Endocr. Metab. 1977, 45, 16–24.

    Article  CAS  Google Scholar 

  • Isomaa V, Isotalo H, Orava M, Jänne O: Regulation of cytosol and nuclear progesterone receptors in rabbit uterus by estrogen, anti-estrogen and progesterone administration. Biochim. Biophys. Acta 1979, 585, 24–33.

    Article  CAS  Google Scholar 

  • Isomaa V, Pajunen A, Bardin C, Jänne O: Nuclear androgen receptors in the mouse kidney: Validation of a new method. Endocrinology 1982, 111, 833–843.

    Article  CAS  Google Scholar 

  • Isomaa V, Orava M, Vihko R: Evidence for an androgen receptor in porcine Leydig cells. Acta Endocrinol. 1987, 115, 119–124.

    Article  CAS  Google Scholar 

  • Jänne O, Apter D, Vihko R: Assay of testosterone, progesterone and 17α-hydroxyprogesterone in human plasma by radioimmunoassay after separation on hydroxyalkoxypropyl Sephadex. J. Steroid Biochem. 1974, 5, 155–162.

    Article  Google Scholar 

  • Jänne O, Kontula K, Vihko R: Progestin receptors in human tissues: concentrations and binding kinetics. J. Steroid Biochem. 1976, 7, 1061–1068.

    Article  Google Scholar 

  • Katzenellenbogen B: Dynamics of steroid hormone action. Ann. Rev. Physiol. 1980, 42, 17–35.

    Article  CAS  Google Scholar 

  • Leavitt W, MacDonald R, Okulicz W: Hormonal regulation of estrogen and progesterone receptor systems. In: Litwack G (ed): Biochemical Action of Hormones. Academic Press, New York 1983, pp. 32–356.

  • Muechler E: The androgen receptor of human endometrium. Endocr. Res. 1987, 13, 69–84.

    Article  CAS  Google Scholar 

  • Prodi G, de Giovanni C, Galli M, Gola G, Grilli S, Rocchetta R, Orlandi C: 17β-estradiol, 5α-dihydrotestosterone, progesterone and Cortisol receptors in normal and neoplastic human endometrium. Tumori 1979, 65, 241–253.

    Article  CAS  Google Scholar 

  • Scatchard G: The attractions of proteins for small molecules and ions. Ann. N.Y. Acad. Sci. 1949, 51, 660–672.

    Article  CAS  Google Scholar 

  • Tamaya T, Motoyama T, Ohono Y, Ide N, Tsurusaki T, Okada H: Estradiol-17β, progesterone and 5α-dihydrotestosterone receptors of uterine myometrium and myoma in the human subject. J. Steroid Biochem. 1979, 10, 615–622.

    Article  CAS  Google Scholar 

  • Tamaya T, Murakami T, Okada H: Concentrations of steroidreceptors in normal human endometrium in relation to the dayof the menstrual cycle. Acta Obstet. Gynecol. Scand. 1986, 65, 195–198.

    Article  CAS  Google Scholar 

  • Walters M, Clark J: Cytosol and nuclear compartmentalization of progesterone receptor of the rat uterus. Endocrinology 1978, 103, 601–609.

    Article  CAS  Google Scholar 

  • Wallenstein S, Zucker CL, Fleiss JL: Some statistical methods useful in circulation research. Circ. Res. 1980, 47, 1–9.

    Article  CAS  Google Scholar 

  • Vesanen M, Isomaa V, Alanko M, Vihko R: Cytosol estrogen and progesterone receptors in bovine endometrium after uterine involution postpartum and in the estrous cycle. Anim. Reprod. Sci. 1988, 17, 9–20.

    Article  CAS  Google Scholar 

  • Vesanen M, Isomaa V, Bolton N, Alanko M, Vihko R: Bovine steroid hormone and SHBG concentrations postpartum and during the oestrous cycle. Acta vet. scand. 1990, 31, 459–569.

    CAS  PubMed  Google Scholar 

  • Vesanen M, Isomaa V, Alanko M, Vihko R: Bovine uterine, cervical and ovarian estrogen and progesterone receptor concentrations. Anim. Reprod. Sci. 1991, 26, 61–71.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Chemistry, University of Oulu, Department of Obstetrics and Gynaecology,, College of Veterinary Medicine, Kajaanintie 50, SF-90220, Hautjärvi, Oulu, Finland

    M. Vesanen, V. Isomaa, M. Alanko & R. Vihko

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  1. M. Vesanen
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  2. V. Isomaa
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  3. M. Alanko
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  4. R. Vihko
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Vesanen, M., Isomaa, V., Alanko, M. et al. Bovine Uterine, Cervical and Ovarian Androgen Receptor Concentrations. Acta Vet Scand 33, 379–386 (1992). https://doi.org/10.1186/BF03547305

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Acta Veterinaria Scandinavica

ISSN: 1751-0147