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Coculture of Bovine Demiembryos Prior to Freezing

»Frysning af halve kvægembryoner efter forudgående dyrkning«.

Abstract

Pregnancy rates after transfer of frozen/thawed bisected embryos, demiembryos, have until now been very low. In the present study it was attempted to improve the freezability rate of demiembryos by culturing them on a monolayer of bovine oviduct epithelial cells (BOEC) prior to freezing. The cultured frozen/thawed demiembryos showed a lower developmental rate than intact embryos. The pregnancy rate (23% ) following transfer was not different from the pregnancy rate after transfer of unfrozen demiembryos (26% ). The calving rate (4%), however, was significantly lower than the calving rate after transfer of fresh demiembryos (23%). Although the overall pregnancy rate achieved in this study was low, it can be concluded that a co-culture period upon BOEC prior to freezing does not improve the viability of frozen demiembryos.

Sammendrag

Halve embryoner, demiembryoner, som fryses, optøs og overføres, giver en meget lav drægtighedsprocent. Formålet med dette forsøg var at undersøge, om det var muligt at forbedre frysbarheden af demiembryoner ved at dyrke dem i 24 t med bovine Ovidukt epithel celler (BOEC). Dette dyrkningssystem skulle give demiembryonerne mulighed for at regenerere og i løbet af dyrkningsperioden forøge celleantallet. På denne måde skulle de gøres mere egnede til at overleve fryseskaderne. Deres viabilitet efter frysning blev vurderet dels ved drægtigheds procent efter overførsel og dels ved udviklingsevne under 24 t genoptaget dyrkning. Ved dyrkning af 243 demiembryoner viste det sig, at de både før og efter frysning havde en dårligere udviklingkapacitet end intakte embryoner.

Ved overførsel af 48 dyrkede og frosne demiembryoner opnåedes en drægtighedsfrekvens på 23%, mens kælvingsfrekvensen kun var på 4%. Ved overførsel af 142 friske demiembryoner var de tilsvarende tal henholdsvis 26% og 23%. Det kunne tolkes således, at demiembryonerne efter dyrkning og frysning har celler nok til at danne fosterblære, men for få til at danne selve fosteret.

Forsøget viste således, at dyrkning med BOEC ikke forbedrede frysbarheden af halve bovine embryoner.

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Correspondence to M. Schmidt.

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Schmidt, M., Smith, S.D., Avery, B. et al. Coculture of Bovine Demiembryos Prior to Freezing. Acta Vet Scand 33, 237–243 (1992). https://doi.org/10.1186/BF03547314

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Keywords

  • frozen half embryos
  • culture
  • BOEC
  • pregnancy rate
  • calving rate