Skip to main content
Download PDF
  • Published:

Determination of Zinc in Pig Blood

Bestimmung des Zinkhaltes in Schweineblut

Bestämning av zinkhalten i helblod från gris

Acta Veterinaria Scandinavica volume 5, pages 7–16 (1964)Cite this article

Summary

A rapid spectrochemical method for determining zinc in 1 ml. portions of pig blood is described. Blood ash is dissolved in HNO3 and excited in a high voltage spark, using graphite capillary electrodes. Spectra are recorded on Kodak SWR or B-10 plates and the analysis (internal standard line pair Zn 2138/Cd 2288) is densitometered.

Some results for young pigs fed on both wet and dry diet show a period of low blood zinc about 13 weeks after birth, coinciding with maximum susceptibility to swine parakeratosis.

Zusammenfassung

Der Zinkhalt im Blut ist mit einer spektrochemischen Methode bestimmt worden. Kleine Mengen Blut — 1 ml — wurden verwendet. Das eingeäscherte Blut wird in HNO3 gelöst mit dem Zusats von Kadmium als Referenz. Die Spektralaufnahme geschieht im Hochspannungsfunken und Spektra werden auf Kodak B-10 Platten registriert. Standardproben werden auf jeder Platte aufgenommen. Die Analyse wird photometriert.

Untersuchungen über den Blutzinkhalt bei Schweinen in einem begrenzten Material von eiweissreicher und eiweissarmer Kost sind beschrieben.

Sammanfattning

Zinkhalten i blod har bestämts med en spektrokemisk metod. Små volymer blod — 1 ml. — har använts. Det inaskade blodet löses i HNO3 med tillsats av kadmium som referens. Spektralupptagningen sker i högspänningsgnista och spektra registreras på Kodak B-10 plåtar. Standardprov upptages på varje plåt. Analysen fotometreras.

Undersökningar över blodzinkhalten hos grisar i ett begränsat material på äggviterik och äggvitefattig utfodring redovisas.

References

  • Eastmond, E. J. and Williams, B. E.: Wide range analysis for zinc using spectrographc line widths. J. Optical Soc. Amer. 1948, 38, 800–803.

    Article  CAS  Google Scholar 

  • Grünberg, W.: Untersuchungen über den Schwermetallgehalt (Kupfer, Zink, Mangan) der Organe, des Rlutes und der Milch beim Schwein. Arck. Exp. Veterinärmedizin 1961, 15, 828–867.

    Google Scholar 

  • Hoch, F. L. and Vallee, B. L.: Precipitation by trichloroacetic acid as a simplification in the determination of zinc in blood and its components. J. Biol. Chem. 1949, 181, 295–306.

    Article  CAS  Google Scholar 

  • Hoekstra, W. G., Lewis, Jr., P. K., Phillips, P. H. and Grummer, R. H.: The relationship of parakeratosis, supplemental calcium and zinc to the zinc content of certain body components of swine. J. Animal Sci. 1956, 15, 752–764.

    Article  CAS  Google Scholar 

  • Huff, L. C.: A sensitive field test for heavy metals in water. Economic Geology 1948, 43, 675–684.

    Article  CAS  Google Scholar 

  • Kägi, J. H. R. and Vallee, B. L.: Determination of zinc by direct extraction from urine with diphenylthiocarbazone. Anal. Chem. 1958, 30, 1951–1954.

    Article  Google Scholar 

  • Middleton, G. and Stuckey, R. E.: Preparation of biological material from metals. I. A critical review of existing procedures. Analyst 1953, 78, 532–542.

    Article  CAS  Google Scholar 

  • Monacelli, R., Tanaka, H. and Yoe, J. H.: Spectrochemical determination of magnesium, chromium, nickel, copper and zinc in human plasma. Clin. Chim. Acta 1956, 1, 577–582.

    Article  CAS  Google Scholar 

  • Månsson, I. and Olsson B.: The intestinal flora of pigs. I. Quantitative studies of coliforms, enterococci, and Clostridia in the faeces of pigs self-fed a high-protein and high-calcium diet. Acta agric. Scand. 1961, XI, 197–210.

  • Månsson, I. and Olsson, B.: Idem. II. Further quantitative studies of coliforms, enterococci, and clostridio in the faeces of pigs self-fed a high-protein and high-calcium diet. Acta agric. Scand. 1961, XI, 257–264.

  • Månsson, I. and Olsson, B.: Idem. VI. Quantitative studies of coliforms, enterococci, and Clostridia in the faeces of pigs fed a high animal protein and calcium diet and determination of histamine and histaminase activity in the blood. Acta agric. Scand. 1962, XII, 344–354.

  • Månsson, I.: Idem. VII. Urinary histamine levels and coliforms, enterococci, and Clostridia in the faeces of pigs fed a high protein diet. Acta agric. Scand. 1963. In press.

  • Paixao, L. M. and Yoe, J. H.: Spectrochemical determination of magnesium, chromium, nickel, copper and zinc in human plasma and red cells. Clin. Chim. Acta 1959, 4, 507–514.

    Article  CAS  Google Scholar 

  • Tucker, H. F. and Salmon, W. D.: Parakeratosis or zinc deficiency disease in pig. Proc. Soc. Exp. Biol. Med. 1955, 88, 613–616.

    Article  CAS  Google Scholar 

  • Thiers, R. E.: Separation, concentration, and contamination, pp. 637– 666 in Yoe, J. H. and Roch, Jr., H. J.: Trace analysis. Symposium Trace Anal. N. Y. Acad. Med. 1955 (Pub. 1957), 672 pp.

  • Vallee, B. L.: Biochemistry, physiology and pathology of zinc. Phys. Rev. 1959, 39, 443–490.

    CAS  Google Scholar 

  • Vallee, B. L. and Gibson, 2nd, J. G.: An improved dithizone method for the determination of small quantities of zinc in blood and tissue samples. J. Biol. Chem. 1948, 176, 435–443.

    Article  CAS  Google Scholar 

  • Vikbladh, I.: Studies on zinc in blood. I. Scand. J. Clin. Lab. Invest. 1950, 2, 143–148.

    Article  CAS  Google Scholar 

  • Zink, T. H.: Vacuum cup electrode for the spectrochemical analysis of solutions. Appl. Spectroscopy 1959, 13, 94–97.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Royal Veterinary College and the Geological Survey of Sweden, Stockholm, Sweden

    I. Månsson, M. Askling & F. Manheim

Authors
  1. I. Månsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Askling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Manheim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Månsson, I., Askling, M. & Manheim, F. Determination of Zinc in Pig Blood. Acta Vet Scand 5, 7–16 (1964). https://doi.org/10.1186/BF03547360

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1186/BF03547360

Acta Veterinaria Scandinavica

ISSN: 1751-0147