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Distribution of Oestrone and Oestradiol in the Bovine Ovary

Verteilung von Oestron und Oestradiol in Rinderovarien

Distribusjonen av østron og østradiol i kuovarier

Summary

The levels of oestrone (oe1) and oestradiol (oe2) were estimated with chemical methods in single bovine ovaries of various categories, including ovaries from different phases of the oestrous cycle and from early pregnancy and in cystic ovaries.

In the luteal tissue the levels of the two oestrogens were generally found to be very low.

In the follicular apparatus (including follicular fluid and residual tissue) the oestrogen contents varied considerably and were higher in the preovulatory ovaries (means: 165 ng. oe1 868 ng. oe2) than in ovaries representing other phases of the oestrous cycle (means during the estimated week 1, 2 and 3 after ovulation respectively: 28 — 18 — 30 ng., oe1 and 91 — 63 — 96 ng. oe2) and from early pregnancy (means: 25 ng. oe1, 42 ng. oe2, foetal length: 2–12 cm.). In the cystic ovaries the contents of oestrogens were higher (means: 230 ng. oe1, 1140 ng. oe2) than in the preovulatory ovaries but the concentrations were lower (means: 45 ng. oe2 per g. as compared to 142 ng. oe2 per g.).

The means of the ratios between oestradiol and oestrone in preovulatory and in cystic ovaries were almost identical (mean percentages oe2 of the sum total oe1 and oe2: 83.3 as compared to 82.5).

The contents of oestrogens (as oe1 + oe2) of the fluid in follicles or cysts were highly correlated with the contents of oestrogens in the corresponding residual tissue (r = 0.90) and on the average about four times as large. The percentages oestradiol of the sum total oestrone and oestradiol in the fluid and in the corresponding residual tissue were also correlated (r = 0.79).

The concentration of oestradiol (and oestrone) in the fluid of follicles and cysts was extremely variable (0.7–600 ng. per ml.) and was apparently not related to the size of the structures except that the concentration in the fluid of preovulatory follicles tended to be higher than in the fluid of small follicles (containing less than 1.5 ml. fluid) and of large follicles or cysts (2–30 ml. fluid).

Zusammenfassung

In Rinderovarien verschiedener Kategorien, darunter Ovarien unterschiedlicher Zyklusphasen, früher Trächtigkeit sowie zystisch entarteter wurde mittels chemischer Methoden der Gehalt an Oestron (oe1) und Oestradiol (oe2) bestimmt.

Der Gehalt der beiden Oestrogene im Lutealgewebe wurde im allgemeinen als niedrig befunden.

Im Follikularapparat (einschließlich Follikeiflüssigkeit und Residualgewebe) variierte der Oestrogengehalt beträchtlich und war in präovulatorischen Ovarien höher (Mittel: 165 ng oe1, 868 ng oe2) als in Ovarien anderer Zyklusphasen (Mittel der geschätzten 1., 2. und 3. Woche nach der Ovulation: 28 — 18 — 30 ng oe1 und 91 — 63 — 96 ng oe2) und in der frühen Trächtigkeit (Mittel: 25 ng oe1, 42 ng oe2, Fruchtlänge 2–12 cm). In den zystisch entarteten Ovarien war der Oestrogengehalt höher (Mittel: 230 ng oe1, 1140 ng oe2) als in den präovulatorischen Ovarien, die Konzentration dagegen niedriger (Mittel: 45 ng oe2 im Vergleich zu 142 ng oe2 per g).

Die Mittelwerte der Verhältnisse zwischen Oestradiol und Oestron waren in den präovulatorischen und in den zystisch entarteten Ovarien fast identisch (oe2 als prozentualer Anteil an der Gesamtmenge oe1 und oe2 betrug durchschnittlich 83,3 im Vergleich zu 82,5).

Die Oestrogenmengen (oe1 + oe2) waren in der Flüssigkeit der Follikel und Zysten mit den Mengen im entsprechenden Residualgewebe hoch korreliert (r = 0,90) und durchschnittlich viermal grösser. Der Gehalt an Oestradiol als prozentualer Anteil an der Gesamtmenge Oestron und Oestradiol in der Follikel- und Zystenflüssigkeit war mit dem des entsprechenden Residualgewebes ebenfalls korreliert (r = 0,79).

Die Konzentration von Oestradiol (und Oestron) in der Follikel-und Zystenflüssigkeit war extrem variabel (0,7–600 ng per ml) und schien in keiner Relation zu der Größe der Strukturen zu stehen, abgesehen von einer Neigung zu höheren Konzentrationen in der Flüssigkeit der präovulatorischen Follikel gegenüber derjenigen in den kleinen (weniger als 1,5 ml Flüssigkeit) und großen Follikeln oder in Zysten (2–30 ml Flüssigkeit).

Sammendrag

Ved hjelp av kjemiske metoder ble innholdet av østron (oe1) og østradiol (oe2) bestemt i kuovarier av forskjeilige kategorier omfattende ovarier fra forskj eilige faser av syklus og fra tidlig drektighet og i systiske ovarier.

Innholdet av de to østrogener i lutealvevet blev vanligvis funnet å være meget lavt.

I follikkelapparatet (follikkelvæske og residualvev) varierte østrogeninnholdet betydelig og var høyere i preovulatoriske ovarier (middel: 165 ng. oe1, 868 ng. oe2) enn i ovarier fra andre faser av syklus (middel i ovarier antatt å være fra henholdsvis 1., 2. og 3. uke etter ovulasjonen: 28 — 18 — 30 ng. oe1 og 91 — 63 — 96 ng. oe2) og fra tidlig drektighet (middel: 25 ng. oel5 42 ng. oe2, fosterlengde 2–12 cm.). I de systiske ovarier var østrogeninnholdet høyere (middel: 230 ng. oe1, 1140 ng. oe2) enn i preovulatoriske ovarier, men konsentrasjonene var lavere (middel: 45 ng. oe2 sammenlignet med 142 ng. oe2 per g.).

Midiene av forholdene mellom østradiol og østron i preovulatoriske og systiske ovarier var nesten identiske (gjennomsnittlig prosent oe2 av summen total oe1 og oe2: 83.3 sammenlignet med 82.5).

Mengdene av østrogener (oe1 + oe2) i væsken av follikler og syster var høyt korrelert med mengdene i korresponderende residualvev (r = 0.90) og var gjennomsnittlig omtrent fire ganger så store. Innholdene av østradiol som prosent av summen total østron og østradiol i væske fra follikler eller syster og i de korresponderende residualvev var også korrelert (r = 0.79).

Konsentrasjonen av østradiol (og østron) i follikkel- og systevæske var ekstremt variabel (0.7–600 ng. per ml.) og syntes ikke å stå i noen relasjon til strukturenes størrelse bortsett fra at konsentrasjonen i væsken fra preovulatoriske follikler tenderte til å være høyere enn i væsken fra både små (mindre enn 1.5 ml. væske) og store follikler eller syster (2–30 ml. væske).

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Lunaas, T. Distribution of Oestrone and Oestradiol in the Bovine Ovary. Acta Vet Scand 5, 35–49 (1964). https://doi.org/10.1186/BF03547363

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