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Determination of Thyroglobulin Antibodies in Obese Strain Chicken Sera Using Chromic Chloride as a Coupling Reagent

Bestämmandet av immunkroppar mot thyroglobulin i sera från höns av Obese-stam med användning av kromklorid som kopplingsagens

Acta Veterinaria Scandinavica volume 14, pages 651–656 (1973)Cite this article

Abstract

Chromic chloride was used for the coupling of chicken thyroid extract on human and chicken erythrocytes. With the coated cells thyroglobulin antibodies were determined in Obese Strain chicken sera. Ultracentrifugation of the sera prior to testing could be omitted. The method is more sensitive, less antigen requiring and less time-consuming than the previous tanned agglutination test.

Sammanfattning

Kromklorid användes för att fästa höns-sköldkörtelextrakt på humana och på hönserythrocyter. Med så preparerade celler bestämdes immunkroppar mot thyroglobulin i sera från höns av Obese-stam. Ultracentrifugering av sera för försöket var onödig. Metoden är känsligare och snabbare och antigenbehovet mindre jämfört med den tidigare tekniken där tanninbehandlade blodkroppar användes.

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Authors and Affiliations

  1. Department of Microbiology and Epizootology, College of Veterinary Medicine, State Veterinary Medical Institute, Helsinki, Finland

    D. Sharma & J. Tuomi

  2. Institute of Animal Breeding, Agricultural Research Centre, Tikkurila, Finland

    D. Sharma & J. Tuomi

Authors
  1. D. Sharma
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  2. J. Tuomi
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Sharma, D., Tuomi, J. Determination of Thyroglobulin Antibodies in Obese Strain Chicken Sera Using Chromic Chloride as a Coupling Reagent. Acta Vet Scand 14, 651–656 (1973). https://doi.org/10.1186/BF03547393

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  • DOI: https://doi.org/10.1186/BF03547393

Keywords

Acta Veterinaria Scandinavica

ISSN: 1751-0147