Stimulation of Heterophil Hemagglutinins in Chickens by a Viral Vaccine and Chicken Thyroid Extract

Abstract

The formation of heteroag-glutinins against human group O, horse and sheep erythrocytes was studied in groups of chickens injected with various materials. Production of antibodies cross-reacting with some or all of the test cell types was induced both with chicken thyroid extract, with or without adjuvant, and with inactivated infectious bronchitis vaccine with adjuvant. The chicken tissue in the injection materials is considered to be responsible, to a great extent, for the stimulations recorded. Also complete Freund’s adjuvant alone, however, had a stimulating effect. The heterogeneity of the antibody response as regards specificity, and differences in the responsiveness of individual chickens, were demonstrated.

Sammendrag

Bildandet av heteroagglutininer mot humana grupp O-, häst- och fårerytrocyter undersöktes i grupper av höns, som injicerats med olika material. Produktionen av immunkroppar, som korsreagerade med en eller flere av testcelltyperna, inducerades av både höns-sköldkörtel-extrakt, med eller utan adjuvans, och av inaktiverat infektiöst bronkitis vaccin tillsammans med adjuvans. Hönsvävnaden i injektions-materialet ansågs till stor del förorsaka de observerade stimulationerna. Emellertid visade också „complete Freund’s“ adjuvans som sådant en stimulerande effekt. Heterogeniteten av immunkroppssvaret med avseende av specificiteten och skillnader i reaktiviteten hos enskilda höns påvisades.