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Proteases of Clostridium Botulinum

V. Studies on the Serological Relationship Between Proteases from Clostridium Botulinum and Other Spore-Forming Bacteria

Clostridium botulinum proteaser

V. Sammenlignende serologiske undersøkelser mellom proteaser produsert av Clostridium botulinum og andre sporedannende bakterier

Acta Veterinaria Scandinavica volume 14, pages 700–711 (1973)Cite this article

Abstract

By the use of the electrophoretic casein precipitating inhibition test (CPI-test) the serological relationship between proteolytic enzymes produced by different species within the genera Clostridium and Bacillus has been tested. The proteases produced by Clostridium botulinum types A, B, C, D and F cross-reacted with each other. Clostridium botulinum strain 84 was inhibited by antiproteases produced against Clostridium sporogenes, Clostridium botulinum types C and F (protease F I and F II), but not by antiproteases against Clostridium botulinum types B and F (protease II), Clostridium bifermentans and Clostridium perfringens. The protease of the newly described Clostridium botulinum strain 89 (type G) was inhibited by Clostridium sporogenes antiprotease, but not by any of the other antiproteases. It is not possible to differentiate between Clostridium botulinum, Clostridium sporogenes and Clostridium perfringens by use of serological differentiation of their proteolytic enzymes. The protease of Clostridium bifermentans is not serologically related to any of the species tested in this investigation. Proteases produced by different Bacilli were not inhibited by antiproteases from Clostridium botulinum types B, C and F, Clostridium sporogenes, Clostridium bifermentans, and the two strains of Clostridium perfringens tested. This investigation indicates a serological relationship between proteases from different Clostridium species, but not a serological relationship between proteases produced by the Clostridium species and Bacillus species tested.

Sammendrag

En har undersøkt det serologiske slektskap hos proteaser produsert av forskjeilige stammer under genus Clostridium og genus Bacillus ved hjelp av antisera produsert på kanin. Ved hjelp av papirelektroforese skilte man de naturlige forekommende inhibitorer og anti-enzymene i serum og videre benyttet en kaseinpresipitasjon-hemmings-reaksjon (CPI-test). Proteaser produsert av Clostridium botulinum type A, B, C, D og F kryssreagerte med hverandre. Clostridium botulinum (stamme 84) ble hemmet av antiproteaser produsert mot Clostridium sporogenes og Clostridium botulinum type C og F (protease F I og F II), men ble ikke hemmet av antiproteaser mot Clostridium botulinum type B og F (protease F II), Clostridium bifermentans og Clostridium perfringens. Clostridium botulinum (stamme 89 G) ble hemmet av Clostridium sporogenes antiprotease, men ellers ikke. Ved hjelp av denne metoden er en ikke i stand til å skille serologisk mellom proteaser av Clostridium perfringens. Clostridium bifermentans protease ble hemmet bare av sitt homologe antiserum, mens Clostridium sporogenes protease kryssreagerte med Clostridium botulinum og Clostridium perfringens antiproteaser, men ikke med Clostridium bifermentans. Proteaser produsert av forskj ellige Bacillus species ble ikke hemmet av antiproteaser mot Clostridium botulinum type B, C og F, Clostridium sporogenes, Clostridium bifermentans og Clostridium perfringens.

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Authors and Affiliations

  1. Norwegian Food Research Institute, Ås, Norway and the Department of Microbiology and Immunology, Veterinary College of Norway, Box 50, 1432 Ås-NLH, Oslo, Norway

    Tore Bjarne Tjaberg & Kåre Fossum

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  1. Tore Bjarne Tjaberg
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  2. Kåre Fossum
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Tjaberg, T.B., Fossum, K. Proteases of Clostridium Botulinum. Acta Vet Scand 14, 700–711 (1973). https://doi.org/10.1186/BF03547399

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Acta Veterinaria Scandinavica

ISSN: 1751-0147