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Peripheral Plasma Levels of Corticosteroids in Normal Beagles and Greyhounds Measured by a Rapid Competitive Protein Binding Technique

Perifera plasmanivåer av corticosteroider hos normala Beagles och Greyhounds mätt med en snabb proteinbindarteknik

Acta Veterinaria Scandinavica volume 14, pages 745–757 (1973)Cite this article

Abstract

A rapid competitive protein binding method for the measurement of peripheral plasma levels of corticosteroids in dogs was evaluated and applied. The rapid method was found to give a minor overestimation of the Cortisol levels as compared to the method including purification of the plasma extract on Sephadex LH-20 columns. In Beagles and Greyhounds mean peripheral plasma levels of corticosteroids of 25.4 and 18.0 ng/ml were found respectively. No diurnal variation of peripheral plasma levels of corticosteroids was obtained. After the intravenous administration of dexamethasone peripheral plasma levels of corticosteroids were found to decrease to about 20 % of the pretreatment levels. When ACTH was administered i.m. a nearly 10 fold increase of the corticosteroid levels was found 60 to 90 min. after the injections. The rapid method described and evaluated should be of value for diagnosis of adrenal syndrome in the dog.

Sammanfattning

En snabb proteinbindarteknik för bestämning av perifera plasmanivåer av corticosteroider hos hund har utvärderats och applicerats. Snabbindartekniken befanns ge en mindre överbestämning av corti-solnivån jämfört med en teknik som inkluderade rening av plasma-extraktet på Sephadex LH-20 kolonner. Hos Beagles och Greyhounds uppmättes en medelplasmanivå av corticosteroider till respektive 25,4 och 18,0 ng/ml. Ingen diurnal variation kunde konstateras. Efter intravenös dexametason injektion sjönk perifera corticosteroidnivån till ungefär 20 procent av nivån som uppmätts före injektionen. Intramuskulär ACTH injektion ökade corticosteroidnivån till nästan det tiodubbla 60 till 90 minuter efter injektionen. Den beskrivna och evaluerade metoden lämpar sig väl för diagnostik av binjurebarkssyndrom hos hund.

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Author notes

  1. M. Richkind

    Present address: Department of Radiology, Nuclear Medicine Division, Center for the Health Sciences, University of California, Los Angeles, USA

Authors and Affiliations

  1. Department of Clinical Biochemistry and the Department of Clinical Radiology, Royal Veterinary College, S-104 05, Stockholm 50, Sweden

    M. Richkind & L. E. Edqvist

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  1. M. Richkind
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  2. L. E. Edqvist
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This investigation was supported by the John M. Olin Foundation.

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Richkind, M., Edqvist, L.E. Peripheral Plasma Levels of Corticosteroids in Normal Beagles and Greyhounds Measured by a Rapid Competitive Protein Binding Technique. Acta Vet Scand 14, 745–757 (1973). https://doi.org/10.1186/BF03547402

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Acta Veterinaria Scandinavica

ISSN: 1751-0147