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The Effects of Peptide-Precipitating Lignosulphonic Acids on the Proteolytic Activity of Pepsin in Vitro and on the Response of Pigs to an Ulcer Inducing Diet

Virkningen av peptidpresipiterende ligninsulfonsyrer på proteolytisk aktivitet av pepsin in vitro og på griser foret med en magesårfremkallende diett

Acta Veterinaria Scandinavica volume 14, pages 44–56 (1973)Cite this article

Abstract

The inhibitory effect of peptide-precipitating lignosulphonic acids on the proteolytic activity of pepsin in vitro has been studied. When an unfractionated lignosulphonic acid preparation was used, total inhibition of the activity of 5 µg crystalline pepsin was obtained by addition of about 15 mg of lignosulphonic acids.

The greatest inhibitory effect was seen with the high-molecular weight lignosulphonic acids, but an effect was also seen with middle-and low-molecular weight lignosulphonic acids.

A reduction in the inhibitory effect was seen when the experiments were performed in the presence of arginine. The inhibition mechanism is discussed in relation to the peptide-precipitating ability of the lignosulphonic acids.

No obvious protection against the appearance of stomach ulcers in four pigs receiving an ulcerogenic diet was seen.

Sammendrag

En har studert virkningen av peptidpresipiterende ligninsulfonsyrer på proteolytisk aktivitet av pepsin in vitro. Når ufraksjonerte ligninsulfonsyrer ble brukt, fikk en fullstendig inhibering av 5 µg krystallinsk pepsin ved tilsetting av 15 mg ligninsulfonsyrer.

Den største inhiberende virkning fant en med de høgmolekylære ligninsulfonsyrer, men det ble også funnet inhiberende virkning av middel- og lavmolekylære ligninsulfonsyrer.

Det ble funnet en reduksjon i den inhiberende virkningen når arginin ble tilsatt. En har diskutert inhiberingsmekanismen i forhold til peptidpresipiterende egenskaper for ligninsulfonsyrer.

Det ble ikke funnet noen tydelig virkning av ligninsulfonsyrer på forekomst av magesår hos 4 griser som ble fóret med en ulcerogen diett.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Veterinary College of Norway, Oslo Dep., Postbox 8146, Oslo 1, Norway

    Bjørn Næss, Ola Westbye, Kjell Ivar Hildrum & Inger Nafstad

  2. Department of Pharmacology and Toxicology, Veterinary College of Norway, Oslo Dep., Postbox 8146, Oslo 1, Norway

    Bjørn Næss, Ola Westbye, Kjell Ivar Hildrum & Inger Nafstad

  3. Department of Pathology, Veterinary College of Norway, Oslo Dep., Postbox 8146, Oslo 1, Norway

    Bjørn Næss, Ola Westbye, Kjell Ivar Hildrum & Inger Nafstad

  4. Food Research Institute, Ås, Norway

    Bjørn Næss, Ola Westbye, Kjell Ivar Hildrum & Inger Nafstad

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  1. Bjørn Næss
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  2. Ola Westbye
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  3. Kjell Ivar Hildrum
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  4. Inger Nafstad
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Næss, B., Westbye, O., Hildrum, K.I. et al. The Effects of Peptide-Precipitating Lignosulphonic Acids on the Proteolytic Activity of Pepsin in Vitro and on the Response of Pigs to an Ulcer Inducing Diet. Acta Vet Scand 14, 44–56 (1973). https://doi.org/10.1186/BF03547409

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Acta Veterinaria Scandinavica

ISSN: 1751-0147