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Immunoglobulins in Normal Porcine Tracheobronchial Secretions

Immunoglobuliner i tracheobronchialsekret hos normala grisar

Abstract

Methods of collecting sputum and tracheobronchial washings are described.

Proteins in normal porcine tracheobronchial secretions are studied by immunochemical qualitative and quantitative methods. Among the immunoglobulins, IgA, IgG and IgM are identified. Immunoelectrophoretic analysis of sputum shows albumin, transferrin and in the α-region 2 components not found in serum. The predominant immunoglobulin in tracheobronchial secretions is IgA, which accounted for about 85 % of the total immunoglobulin concentration in sputum and 61 % of the immunoglobulin concentration in tracheobronchial washings.

Sammanfattning

Metoder att samla sputum och tracheobronchial spolvätska beskrivs. Proteiner i normala tracheobronchiala sekret hos svin studeras med immunokemiska kvalitativa och kvantitativa metoder. Bland immunoglobulinerna identifieras IgA, IgG och IgM. Immunoelektroforetisk analys av sputum visar albumin, transferrin och i α-regionen två komponenter vilka ej kan påvisas i serum. Det dominerande immunoglobulinet i tracheobronchiala sekret är IgA, som utgör cirka 85 % av den totala immunoglobulinkoncentrationen i sputum och cirka 61 % av totala immunoglobulinkoncentrationen i tracheobronchial spolvätska.

References

  • Anzai, T., J. Ibayashi, C. M. Carpenter & L. Hyde: β2a-globulin as a molecular constituent of insoluble bronchial mucus gel. Amer. Rev. resp. Dis. 1963, 88, 503–508.

    CAS  PubMed  Google Scholar 

  • Bourne, F. J.: IgA immunoglobulin from porcine milk. Biochim. biophys. Acta (Amst.) 1969, 181, 485–487.

    Article  CAS  Google Scholar 

  • Bourne, F. J., J. Pickup & J. W. Honor: Intestinal immunoglobulins in the pig. Biochim. biophys. Acta (Amst.) 1971, 229, 18–25.

    Article  CAS  Google Scholar 

  • Chodirker, W. B. & T. B. Tomasi: Gammaglobulins: Quantitative relationships in human serum and nonvascular fluids. Science 1963, 142, 1080–1081.

    Article  CAS  Google Scholar 

  • Hanson, L. A.: Comparative immunological studies of the immunoglobulins of human milk and blood serum. Int. Arch. Allergy 1961, 18, 241–267.

    Article  CAS  Google Scholar 

  • Jonsson, Å.: to be published.

  • Keimowitz, R. I.: Immunoglobulins in normal human tracheobronchial washings. A qualitative and quantitative study. J. Lab. clin. Med. 1964, 63, 54–59.

    CAS  PubMed  Google Scholar 

  • Lieberman, P., J. Ricks, L. W. Chakim, J. R. Wardell & R. Patterson: Immunoglobulins in respiratory secretions obtained from the canine tracheal pouch. Proc. Soc. exp. Biol. (N.Y.) 1970, 135, 713–716.

    Article  CAS  Google Scholar 

  • Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall: Protein measurement with the Folin fenol reagent. J. biol. Chem. 1951, 193, 265–275.

    Article  CAS  Google Scholar 

  • Mancini, G., A. O. Corbonara & J. F. Heremans: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 1965, 2, 235–254.

    Article  CAS  Google Scholar 

  • Martinsson, K.: Immunoglobulin therapy in piglets. A methodological and experimental study. Acta vet. scand. 1970, Suppl. 29.

  • Masson, P. L., J. F. Heremans & J. Prignot: Studies on the proteins of human bronchial secretions. Biochim. biophys. Acta (Amst.) 1965, 111, 466–478.

    Article  CAS  Google Scholar 

  • Porter, P.: Transfer of immunoglobulins IgG, IgA and IgM to lacteal secretions in the parturient sow and their absorption by the neonatal piglet. Biochim, biophys. Acta (Amst) 1969, 181, 381–392.

    Article  CAS  Google Scholar 

  • Porter, P., D. E. Nookes & W. D. Allen: Secretory IgA and antibodies to Escherichia coli in porcine colostrum and milk and their significance in the alimentary tract of the young pig. Immunology 1970, 18, 245–257.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rasche, B. & W. T. Ullmer: Untersuchungen über die Zusammensetzung des Bronchialschleimes bei chronisch obstruktiver Bronchitis. (The composition of bronchial mucus in chronic-obstructive bronchitis). Pneumonologi 1971, 144, 10–32.

    Article  CAS  Google Scholar 

  • Richardson, A.K. & P.C. Kelleher: The 11 S sow colostral lg. Isolation and physico-chemical properties. Biochim. biophys. Acta (Amst.) 1970, 214, 117–124.

    Article  CAS  Google Scholar 

  • Scheidegger, J. J.: Une microméthode de rimmuno-électrophorèse. (A micro-method of immuno-electrophoretic analysis). Int. Arch. Allergy 1955, 7, 103–110.

  • Tomasi, T. B., E. M. Tan, A. Solomon & A. Prendergast: Characteristics of an immune system common to certain external secretions. J. exp. Med. 1965, 121, 101–125.

    Article  CAS  Google Scholar 

  • Tomasi, T. B. & J. Bienenstock: Secretory immunoglobulins. Advanc. Immunol. 1968, 9, 1–96.

    Article  CAS  Google Scholar 

  • Turgeon, P., F. Turgeon & J. Robert: Les immunoglobulines sécrétoires trachéo-bronchiques. (Immunoglobulins in tracheobronchial fluids). Un. méd. Can. 1969, 98, 934–939.

    Google Scholar 

  • Turgeon, P., J. Robert & F. Turgeon: Étude des immunoglobulines sériques et sécrétoires trachéo-bronchiques. (Study of serum and secretory tracheobronchial immunoglobulins). Un. méd. Can. 1971, 100, 232–239.

    CAS  Google Scholar 

  • Vaerman, J. P., J. B. Arbuckle & J. F. Heremans: Immunoglobulin A in the pig. II. Sow’s colostral and milk IgA: Quantitative studies and molecular size estimation. Int. Arch. Allergy 1970, 39, 323–333.

    Article  CAS  Google Scholar 

  • Wadsworth, C.: A slide microtechnique for the analysis of immune precipitins in gel. Int. Arch. Allergy 1957, 10, 355–360.

    Article  CAS  Google Scholar 

  • Waldman, R. H., J. J. Mann & J. A. Kasel: Influenza virus neutralizing antibody in human respiratory secretions. J. Immunol. 1968, 100, 80–85.

    CAS  PubMed  Google Scholar 

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Supported by the Swedish Council for Forestry and Agricultural Research.

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Holmgren, N. Immunoglobulins in Normal Porcine Tracheobronchial Secretions. Acta Vet Scand 14, 366–380 (1973). https://doi.org/10.1186/BF03547425

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