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The Hypocalcemic Response to Protamine as a Measure of Bone Resorption

Det hypokalcemiska svaret på protamininjektion som ett matt på benresorbtionen

Acta Veterinaria Scandinavica volume 14, pages 428–435 (1973)Cite this article

Abstract

The heparin antagonist protamine inhibits bone resorption. In the present study protamine was given i.v. at a dose of 10 mg/kg to goats and sheep in various stages of pregnancy and lactation. It is known that bone resorption increases as pregnancy progresses. In sheep maximum in bone resportion is reached at early lactation. The hypocalcemic response to protamine followed a similar course. The slightest decrease in serum calcium was obtained during early pregnancy and the greatest during late pregnancy and early lactation. Protamine increased serum inorganic phosphorus. The increase was most pronounced during early pregnancy. Protamine did not significantly change serum magnesium.

Five lactating ewes were given 25000 i.u./kg of vitamin D3. Serum calcium increased and the hypocalcemic response to protamine was greater after vitamin D3 administration than before.

The results showed that the hypocalcemic response to protamine can be used as an indicator of the role of bone resorption in calcium homeostasis in various physiological situations.

Sammanfattning

Heparinantagonisten protamin blockerar benresorbtionen. Protamin gavs intravenöst (10 mg/kg) till får och getter i olika dräktighets- och laktationsstadier. Det är känt att benresorbtionen ökar allt eftersom dräktigheten framskrider. Hos får når benresorbtionen maximum under tidig laktation. Den minsta sänkningen av serumkalcium efter protamininjektion erbölls under tidig dräktighet och den största under sen dräktighet och tidig laktation.

Protamin ökade serumkoncentrationen av oorganisk fosfor, ökningen var mest påtaglig under tidig dräktighet.

Serumkoncentrationen av magnesium förblev i stort sett oförändrad efter protamininjektion.

Fem lakterande tackor behandlades med 25000 I.E. vitamin D3/kg. Serumkoncentrationen av kalcium ökade, och det hypocalcemiska svaret på protamininjektion var större efter D-vitamin behandlingen.

Resultaten visade att protamin kan användas för att studera den roll benresorbtionen spelar i kalciumhomeostasen under olika fysiologiska förhållanden.

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  1. Department of Medicine II, Royal Veterinary College, S-104 05, Stockholm 50, Sweden

    Jan Luthman, Gösta Jonson & Jan Persson

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  1. Jan Luthman
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  2. Gösta Jonson
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  3. Jan Persson
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Luthman, J., Jonson, G. & Persson, J. The Hypocalcemic Response to Protamine as a Measure of Bone Resorption. Acta Vet Scand 14, 428–435 (1973). https://doi.org/10.1186/BF03547430

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Acta Veterinaria Scandinavica

ISSN: 1751-0147