- Published:
Bovine Mycoplasmas: Cultural and Biochemical Studies II
Bovine mykoplasmer: Dyrkningsmæssige og biokemiske undersøgelser. II.
Acta Veterinaria Scandinavica volume 14, pages 450–463 (1973)
Abstract
A number of biochemical and biological tests have been performed using reference strains of presently known mycoplasma species or sero-groups of bovine origin. The purpose of these investigations was partly to fulfil the requirements of “The Subcommittee on the Taxonomy of Mycoplasmatares” in describing new species of mycoplasmas, and partly to select methods which might be of value in daily diagnostic work. Concerning the latter point, the following tests are recommended for strains belonging to the digitonin resistant genus Acholeplasma: catabolism of galactose, xylose, aesculin and arbutin. In the genus Mycoplasma, which is digitonin sensitive, 5 tests are of special value: catabolism of glucose and arginine, phosphatase activity, formation of “film and spots”, and serum digestion.
Sammendrag
Referencestammer for de for tiden kendte bovine mykoplasmaarter eller serogrupper er undersøgt ved en række biokemiske og biologiske tests. Formålet med disse undersøgelser var dels at opfylde de krav, som er opstillet af „The Subcommittee on the Taxonomy of Mycoplasmatales“ ved beskrivelse af nye arter, og dels at udvælge metoder, som er af værdi i det daglige diagnostiske arbejde. I relation til det sidste punkt anbefales følgende undersøgelser for stammer tilhørende slægten Acholeplasma, som er digitonin resistent: Katabolisme af galaktose, xylose, aesculin og arbutin. For genus Mycoplasma, som er digitonin følsom, er følgende 5 prøver af særlig værdi: Katabolisme af glukose og arginin, phosphataseaktivitet, dannelse af “film and spots” og serumspaltning.
References
Al-Aubaidi, J. M. & J. Fabricant: Characterization and classification of bovine mycoplasma. Cornell Vet. 1971, 61, 490–518.
Aluotto, B. B., R. G. Wittler, C. O. Williams & J. E. Faber: Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. system. Bact. 1970, 20, 35–58.
Barber, T. L. & J. Fabricant: Identification of Mycoplasmatales: Characterization procedures. Appl. Microbiol. 1971, 21, 600–605.
Clyde, W. A.: Hemolysis in identifying Eatons’ pleuropneumonia-like organism. Science 1963, 139, 55.
Edwards, D. G. ff.: An investigation of the biological properties of organisms of the pleuropneumonia group, with suggestions regarding the identification of strains. J. gen. Microbiol. 1950, 4, 311–329.
Edward, D. G. ff. & E. A. Freundt: Classification of the Mycoplasmatales. In The Mycoplasmatales and the L-phase of Bacteria. L. Hayflick ed., Appleton-Century-Crofts, New York 1969, 187– 190.
Ernø, H. & L. Stipkovits: Bovine mycoplasmas: Cultural and biochemical studies. I. Acta vet. scand. 1973, 14, 436–449.
Fabricant, J. & E. A. Freundt: Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann. N.Y. Acad. Sci. 1967, 143, 50–58.
Manchee, R. J. & D. Taylor-Robinson: Hemadsorption and hemagglutination by mycoplasmas. J. gen. Microbiol. 1968, 50, 465–478.
Mensik, J. & K. Jurmanova: Occurrence of Mycoplasma bovirhinis in connection with pneumonia of calves. Abstr. Xth Conf. Tax. Bact. Mycopl., Brno 1970, 10–11.
Razin, S. & R. C. Cleverdon: Carotenoids and cholesterol in membranes of Mycoplasma laidlawii. J. gen. Microbiol. 1965, 41, 409–415.
Rothblat, G. H. & P. F. Smith: Nonsaponifiable lipids of representative pleuropneumonia-like organisms. J. Bact. 1961, 82, 479– 491.
Rottem, S. & S. Razin: Electrophoretic patterns of membrane proteins of mycoplasma. J. Bact. 1967, 94, 359–364.
Sander, T.: Personal communication 1972.
Subcommittee on the Taxonomy of Mycoplasmatales: Proposal for minimal standards for descriptions of new species of the order Mycoplasmatales. Int. J. system. Bact. 1972, 22, 184–188.
Taylor-Robinson, D., N. L. Somerson, H. C. Turner & R. M. Chanock: Serological relationships among human mycoplasmas as shown by complement-fixation and gel diffusion. J. Bact. 1963, 85, 1261–1273.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ernø, H., Stipkovits, L. Bovine Mycoplasmas: Cultural and Biochemical Studies II. Acta Vet Scand 14, 450–463 (1973). https://doi.org/10.1186/BF03547432
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1186/BF03547432