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Diagnosis of Mixed Infections With Myxovirus Influenzae a Equi 2 and Herpes Virus Equi 1 Among Danish Stud Horses

Diagnostiske resultater med blandingsinfektioner med Myxouirus influenzae A equi 2 og Herpesvirus equi 1 i nogle danske hestebesætninger.

Acta Veterinaria Scandinavica volume 14, pages 205–218 (1973)Cite this article

Abstract

Examination of nasopharyngeal secretion and organ material from clinical cases of respiratory diseases in horses, using inoculation of embryonated hen eggs and rabbit and horse kidney cell cultures, resulted in the isolation of influenza virus and herpes virus. In 2 cases, both viruses were present in the same specimen.

On the basis of the physio-chemical, cytological and serological criteria, the viruses were found to be identical with influenza virus type A equi 2 and herpes virus equi type 1.

The methods for serological diagnosis and characterization of the influenza and herpes viruses are discussed. Detailed serological examinations of blood specimens from the clinical cases confirmed the aetiological significance of the isolates and the presence of mixed infections.

Further characterization of a strain of influenza virus was carried out by inoculation experiments on horses. These included reproduction of clinical influenza, reisolation of the strain in casu and a study of the antibody formation.

Sammendrag

Fra nasopharyngeal sekret og organmateriale fra kliniske tilfælde af respirationsvejslidelser hos heste er der efter podning med materialet på embryonerede hønseæg og kanin- og hestenyrecellekulturer isoleret influenzavirus og herpesvirus. I to tilfælde påvistes begge virus i det samme prøvemateriale.

Ud fra fysisk-kemiske, cytologiske og serologiske kriterier er de to virus fundet identiske med influenzavirus type A equi 2 og herpesvirus equi type 1.

Metoder til serologisk diagnostik og karakteristik af influenzavirus og herpesvirus diskuteres.

Med en influenzavirusstamme er der udført podningsforsøg på heste. Herunder fremkaldtes klinisk influenza, og virusstammen in casu isoleredes. Der udførtes endvidere studier over antistofdannelsen.

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  1. State Veterinary Institute for Virus Research, Kalvehave, Lindholm, Denmark

    Kurt Jensen

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Jensen, K. Diagnosis of Mixed Infections With Myxovirus Influenzae a Equi 2 and Herpes Virus Equi 1 Among Danish Stud Horses. Acta Vet Scand 14, 205–218 (1973). https://doi.org/10.1186/BF03547437

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Acta Veterinaria Scandinavica

ISSN: 1751-0147